Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,810,689	T→C	V160A (GTA→GCA)	ydjM →	putative inner membrane protein regulated by LexA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,810,689	0	T	C	100.0%	181.5 / NA	51	V160A (GTA→GCA)	ydjM	putative inner membrane protein regulated by LexA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/27);  total (24/27)

TGCCGATATGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGG  >  NC_000913/1810555‑1810824                                                                                                                                       |                                                                                                                                        tGCCGATATGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGa                                                                                                                                    <  2:363266/140‑1 (MQ=255)     gATATGCTGACCCCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGcc                                                                                                                                <  2:632689/140‑1 (MQ=255)     gATATGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGcc                                                                                                                                >  2:842868/1‑140 (MQ=255)      atatGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGccc                                                                                                                               >  1:798868/1‑140 (MQ=255)      atatGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGccc                                                                                                                               <  1:84705/140‑1 (MQ=255)      atatGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGccc                                                                                                                               <  1:681954/140‑1 (MQ=255)             gACCCCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCcattcat                                                                                                                        <  1:72018/140‑1 (MQ=255)                    gccgGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGACATGGATGCCCCATTCATTACCCga                                                                                                                 >  1:1099767/1‑140 (MQ=255)                     ccgGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCgag                                                                                                                >  2:310997/1‑140 (MQ=255)                            tCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAgcg                                                                                                         >  1:842214/1‑140 (MQ=255)                            tCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAgcg                                                                                                         >  2:1176029/1‑140 (MQ=255)                              cccTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCt                                                                                                       <  1:598390/140‑1 (MQ=255)                                  gCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTc                                                                                                   <  2:691249/140‑1 (MQ=255)                                   ctctGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCg                                                                                                  >  2:694277/1‑140 (MQ=255)                                           aTGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCAt                                                                                          <  1:842868/140‑1 (MQ=255)                                                 cTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCaaa                                                                                    <  1:310997/140‑1 (MQ=255)                                                  tGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAAt                                                                                   >  2:161121/1‑140 (MQ=255)                                                    gCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGa                                                                                 >  2:141166/1‑140 (MQ=255)                                                       tttCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGACATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCa                                                                              <  2:1099767/140‑1 (MQ=255)                                                          ccGCTTGCCTATCCTGGTT‑CCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATAc                                                                          <  1:506936/140‑1 (MQ=255)                                                               tGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATTCCTTg                                                                      <  1:769164/140‑1 (MQ=255)                                                                 ccTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCa                                                                    <  1:1176029/140‑1 (MQ=255)                                                                     tCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATa                                                                           >  2:976818/1‑129 (MQ=255)                                                                     tCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATa                                                                           <  1:976818/129‑1 (MQ=255)                                                                          gTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGtt                                                           >  2:1042389/1‑140 (MQ=255)                                                                               ccAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATc                                                      >  2:663327/1‑140 (MQ=255)                                                                                 aaaaGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCgg                                                    <  1:161121/140‑1 (MQ=255)                                                                                  aaaGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGc                                                   <  2:682469/140‑1 (MQ=255)                                                                                   aaGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCt                                                  <  1:1042389/140‑1 (MQ=255)                                                                                        caaccaacTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCttatta                                             >  2:870495/1‑140 (MQ=255)                                                                                        caaccaacTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCttatta                                             >  2:823902/1‑140 (MQ=255)                                                                                         aaccaacTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTaa                                            >  1:417982/1‑140 (MQ=255)                                                                                            caacTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCa                                         <  1:823902/140‑1 (MQ=255)                                                                                                 ggAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAGCAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAgg                                    <  1:66598/140‑1 (MQ=255)                                                                                                   aaCGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCCTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGtt                                  <  2:533278/140‑1 (MQ=255)                                                                                                     cGTTTTATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGa                                >  2:303946/1‑140 (MQ=255)                                                                                                       ttttATCTGCATGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAAt                              <  1:303946/140‑1 (MQ=255)                                                                                                                 aTGGCATTATTTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAgg                    >  2:874792/1‑140 (MQ=255)                                                                                                                      attattTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCaaaa               >  2:229009/1‑140 (MQ=255)                                                                                                                      attattTGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCaaaa               >  2:775230/1‑140 (MQ=255)                                                                                                                        tattTGTCTGGTCTGCATGGACGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGGTGAATACTAAAAAGGCAAAAAt             >  2:693188/1‑140 (MQ=255)                                                                                                                          tttGTCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCa           <  2:876940/140‑1 (MQ=255)                                                                                                                              tCTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCtt       >  2:648287/1‑140 (MQ=255)                                                                                                                               cTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCttt      <  2:599907/140‑1 (MQ=255)                                                                                                                               cTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCttt      <  1:775230/140‑1 (MQ=255)                                                                                                                               cTGGTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCttt      <  1:229009/140‑1 (MQ=255)                                                                                                                                  gTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTg   >  1:432974/1‑140 (MQ=255)                                                                                                                                  gTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTg   <  2:798868/140‑1 (MQ=255)                                                                                                                                  gTCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTg   >  1:824743/1‑140 (MQ=255)                                                                                                                                   tCGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTgg  >  2:970972/1‑140 (MQ=255)                                                                                                                                    cGGCATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCAc          <  1:127180/131‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        TGCCGATATGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGG  >  NC_000913/1810555‑1810824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑