Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,974,541	T→C	T262A (ACC→GCC)	cheA ←	chemotaxis protein CheA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,974,541	0	T	C	96.8%	209.6 / ‑2.1	63	T262A (ACC→GCC)	cheA	chemotaxis protein CheA
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (37/24);  total (38/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

TTATCAAATCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTC  >  NC_000913/1974412‑1974676                                                                                                                                  |                                                                                                                                        ttATCAAATCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATtg                                                                                                                               >  2:1180216/1‑140 (MQ=255)        aTCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGcgcg                                                                                                                        <  1:214345/140‑1 (MQ=255)          cACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGcgtc                                                                                                                      >  2:1150620/1‑140 (MQ=255)          cACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGcgtc                                                                                                                      >  2:348993/1‑140 (MQ=255)               tGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGtttt                                                                                                                 >  2:643475/1‑140 (MQ=255)                  ttAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTc                                                                                                              >  2:769956/1‑140 (MQ=255)                    aaCCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTccc                                                                                                            >  2:326631/1‑140 (MQ=255)                    aaCCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTccc                                                                                                            >  1:83687/1‑140 (MQ=255)                     aCCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTcc                                                                                                             <  2:306438/138‑1 (MQ=255)                     aCCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTcc                                                                                                             >  1:306438/1‑138 (MQ=255)                     aCCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCg                                                                                                           <  2:449080/140‑1 (MQ=255)                      ccGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGc                                                                                                          >  1:765067/1‑140 (MQ=255)                         ggTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTcc                                                                                                       >  2:1005556/1‑140 (MQ=255)                          gTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCa                                                                                                      >  1:93856/1‑140 (MQ=255)                           tCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCAc                                                                                                     >  2:475511/1‑140 (MQ=255)                                ttCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGc                                                                                                >  2:328207/1‑140 (MQ=255)                                ttCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGc                                                                                                <  1:328207/140‑1 (MQ=255)                                ttCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGc                                                                                                <  1:1180216/140‑1 (MQ=255)                                    cTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGt                                                                                            >  1:177311/1‑140 (MQ=255)                                    cTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGt                                                                                            >  1:9114/1‑140 (MQ=255)                                          cGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGgcgc                                                                                      >  2:717358/1‑140 (MQ=255)                                                 cAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGt                                                                                            <  1:49902/127‑1 (MQ=255)                                                 cAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGt                                                                                            >  2:49902/1‑127 (MQ=255)                                                     cATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTg                                                                           <  2:177311/140‑1 (MQ=255)                                                         gACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAg                                                                       >  1:1181888/1‑140 (MQ=255)                                                              ggTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGttt                                                                    >  1:1058378/1‑138 (MQ=255)                                                              ggTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGttt                                                                    <  2:1058378/138‑1 (MQ=255)                                                              ggTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTaa                                                                  >  1:721181/1‑140 (MQ=255)                                                              ggTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTa                                                                   >  2:717977/1‑139 (MQ=255)                                                              ggTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTa                                                                   <  1:717977/139‑1 (MQ=255)                                                               gTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAg                                                                 <  2:899629/140‑1 (MQ=255)                                                                 gATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCa                                                               <  1:769956/140‑1 (MQ=255)                                                                      ccAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACtggt                                                          <  2:765067/140‑1 (MQ=255)                                                                       cAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACtggtg                                                         <  2:93856/140‑1 (MQ=255)                                                                            cGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTggtggggtg                                                    <  1:348993/140‑1 (MQ=255)                                                                                        taattaatTGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGc                                        <  1:475511/140‑1 (MQ=255)                                                                                          attaattGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCga                                      <  2:187286/140‑1 (MQ=255)                                                                                                tGATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTc                                >  2:126464/1‑140 (MQ=255)                                                                                                 gATCAACCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCt                               >  1:604531/1‑140 (MQ=255)                                                                                                     aaCCTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTg                           >  2:119150/1‑140 (MQ=255)                                                                                                       ccTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGtt                         >  2:256124/1‑140 (MQ=255)                                                                                                        cTTTTCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGttt                        <  1:1005556/140‑1 (MQ=255)                                                                                                           ttCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCaa                     <  2:1181888/140‑1 (MQ=255)                                                                                                            tCTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCaaa                    >  1:1113002/1‑140 (MQ=255)                                                                                                             cTACCGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAg                   <  1:119150/140‑1 (MQ=255)                                                                                                                 cGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGttt                        <  1:1002721/131‑1 (MQ=255)                                                                                                                 cGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGttt                        >  2:1002721/1‑131 (MQ=255)                                                                                                                 cGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTaa               >  1:960474/1‑140 (MQ=255)                                                                                                                 cGCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTaa               >  1:288937/1‑140 (MQ=255)                                                                                                                  gCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAAt              >  2:383661/1‑140 (MQ=255)                                                                                                                  gCTACACGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAAt              >  2:1097179/1‑140 (MQ=255)                                                                                                                     acacGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTg           >  1:667687/1‑140 (MQ=255)                                                                                                                      cacGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGa          <  2:721181/140‑1 (MQ=255)                                                                                                                       acGGATGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGAt         <  2:288937/140‑1 (MQ=255)                                                                                                                           aTGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGTGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGc     >  2:335316/1‑140 (MQ=255)                                                                                                                           aTGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGa          >  1:817194/1‑135 (MQ=255)                                                                                                                           aTGCTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGa          <  2:817194/135‑1 (MQ=255)                                                                                                                              cTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGAt         <  2:628588/133‑1 (MQ=255)                                                                                                                              cTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGAt         >  1:628588/1‑133 (MQ=255)                                                                                                                              cTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTc  <  2:415191/140‑1 (MQ=255)                                                                                                                              cTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTc  >  2:545217/1‑140 (MQ=255)                                                                                                                              cTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTc  >  1:415191/1‑140 (MQ=255)                                                                                                                              cTGGCGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTc  >  2:933439/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                        TTATCAAATCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTC  >  NC_000913/1974412‑1974676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑