Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,083,073	T→C	R106R (CGT→CGC)	sbcB →	exodeoxyribonuclease I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,083,073	0	T	C	100.0%	168.2 / NA	48	R106R (CGT→CGC)	sbcB	exodeoxyribonuclease I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/25);  total (23/25)

CAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGA  >  NC_000913/2082937‑2083208                                                                                                                                         |                                                                                                                                        cagGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTc                                                                                                                                      <  1:1078618/140‑1 (MQ=255)             taattaCCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTc                                                                                                                          <  2:665011/140‑1 (MQ=255)                  aCCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACg                                                                                                                     <  1:1182929/140‑1 (MQ=255)                  aCCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACg                                                                                                                     <  1:732864/140‑1 (MQ=255)                    cGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCa                                                                                                                   <  2:269290/140‑1 (MQ=255)                    cGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCa                                                                                                                   <  2:778407/140‑1 (MQ=255)                      gTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAAc                                                                                                                 >  2:262765/1‑140 (MQ=255)                           aCCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACAtttt                                                                                                            >  2:1060158/1‑140 (MQ=255)                               cGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTAt                                                                                                        <  1:340725/140‑1 (MQ=255)                               cGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTAt                                                                                                        >  2:617293/1‑140 (MQ=255)                               cGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTAt                                                                                                        >  2:1000507/1‑140 (MQ=255)                                 cAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCg                                                                                                      >  2:697118/1‑140 (MQ=255)                                 cAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCg                                                                                                      >  1:488617/1‑140 (MQ=255)                                 cAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCg                                                                                                      >  1:723688/1‑140 (MQ=255)                                 cAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCg                                                                                                      >  2:990366/1‑140 (MQ=255)                                    gAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTaa                                                                                                   <  2:488617/140‑1 (MQ=255)                                      aGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAtt                                                                                                 >  2:630040/1‑140 (MQ=255)                                        cACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTAt                                                                                                        >  1:312554/1‑131 (MQ=255)                                        cACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTAt                                                                                                        <  2:312554/131‑1 (MQ=255)                                            ggCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACg                                                                                           >  1:716561/1‑140 (MQ=255)                                                 aaGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCt                                                                                      <  2:275121/140‑1 (MQ=255)                                                      gaAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGc                                                                                 <  1:697118/140‑1 (MQ=255)                                                       aaaaCGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGcc                                                                                >  2:276747/1‑140 (MQ=255)                                                              aGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCt                                                                         <  1:630040/140‑1 (MQ=255)                                                              aGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCt                                                                         >  2:1029842/1‑140 (MQ=255)                                                                  gcgTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGgca                                                                     <  1:990366/140‑1 (MQ=255)                                                                             ccGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACt                                                          <  1:1000507/140‑1 (MQ=255)                                                                                       tCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACt                                                          >  1:187067/1‑130 (MQ=255)                                                                                       tCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACt                                                          <  2:187067/130‑1 (MQ=255)                                                                                       tCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCTGGGa                                                <  1:276747/140‑1 (MQ=255)                                                                                        cGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCTGGGAt                                               <  1:332606/140‑1 (MQ=255)                                                                                            ttttACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCTGGGATTTAc                                           <  1:507137/140‑1 (MQ=255)                                                                                                       cgAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCTGGGATTTACTGGATGTTATg                                <  1:1060158/140‑1 (MQ=255)                                                                                                            aCCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCTGGGATTTACTGGATGTTATGCGTGc                           <  1:1028130/140‑1 (MQ=255)                                                                                                                 tATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCTGGGATTTACTGGATGTTATGCGTGCCTGtt                      >  1:1116743/1‑140 (MQ=255)                                                                                                                      tGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGcc                 >  1:330113/1‑140 (MQ=255)                                                                                                                        ggCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCt               >  2:377261/1‑140 (MQ=255)                                                                                                                        ggCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCt               <  2:723688/140‑1 (MQ=255)                                                                                                                           tACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCTGGGATTTACTGGATGTTATGCGTGc                           <  2:1052984/125‑1 (MQ=255)                                                                                                                           tACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCTGGGATTTACTGGATGTTATGCGTGc                           >  1:1052984/1‑125 (MQ=255)                                                                                                                             caacaaTGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGcc          >  1:873430/1‑140 (MQ=255)                                                                                                                             caacaaTGTGCGCTTCGACGACCAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGcc          >  1:945504/1‑140 (MQ=255)                                                                                                                                caaTGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCgg       <  1:964256/140‑1 (MQ=255)                                                                                                                                 aaTGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCgga      <  1:578997/140‑1 (MQ=255)                                                                                                                                  aTGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTgcgc           <  2:464077/134‑1 (MQ=255)                                                                                                                                  aTGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTgcgc           >  1:464077/1‑134 (MQ=255)                                                                                                                                   tgtgCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCTGGGATTTACTGGATGATATGCGTGCCTGTTATGCCCTGCGCCCggaag    >  2:103498/1‑140 (MQ=255)                                                                                                                                     tgCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCggaagga  >  2:152377/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        CAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGA  >  NC_000913/2082937‑2083208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑