Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,095,988	T→C	F49L (TTC→CTC)	hisF →	imidazole glycerol phosphate synthase subunit HisF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,095,988	0	T	C	100.0%	192.3 / NA	54	F49L (TTC→CTC)	hisF	imidazole glycerol phosphate synthase subunit HisF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/23);  total (31/23)

AAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCT  >  NC_000913/2095853‑2096121                                                                                                                                        |                                                                                                                                      aaaCGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTa                                                                                                                                   <  1:32674/140‑1 (MQ=255)     gCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGat                                                                                                                               <  1:751950/140‑1 (MQ=255)        tAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATc                                                                                                                            >  2:579853/1‑140 (MQ=255)          aTCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCAc                                                                                                                          <  1:78549/140‑1 (MQ=255)              cATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCt                                                                                                                      >  2:180530/1‑140 (MQ=255)               aTGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCtt                                                                                                                     <  1:78573/140‑1 (MQ=255)                tGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTc                                                                                                                    >  2:809773/1‑140 (MQ=255)                 gTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTcc                                                                                                                   >  2:152998/1‑140 (MQ=255)                     cGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCg                                                                                                               >  1:454137/1‑140 (MQ=255)                     cGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCg                                                                                                               >  1:1160226/1‑140 (MQ=255)                      gACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGa                                                                                                              >  2:234168/1‑140 (MQ=255)                         gTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATgg                                                                                                           <  1:579853/140‑1 (MQ=255)                           tCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGcc                                                                                                         <  1:144870/140‑1 (MQ=255)                           tCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGACAAAACGCCACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGcc                                                                                                         >  1:251329/1‑140 (MQ=255)                                 tGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTgg                                                                                                   <  1:784456/140‑1 (MQ=255)                                 tGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTgg                                                                                                   <  2:307525/140‑1 (MQ=255)                                     cAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCgtg                                                                                                      <  1:129352/133‑1 (MQ=255)                                     cAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCgtg                                                                                                      >  2:129352/1‑133 (MQ=255)                                     cAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGa                                                                                               >  2:252483/1‑140 (MQ=255)                                      aGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGAt                                                                                              <  1:185901/140‑1 (MQ=255)                                      aGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGAt                                                                                              <  1:131516/140‑1 (MQ=255)                                       ggtggtGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATa                                                                                             >  1:1182619/1‑140 (MQ=255)                                          ggtgAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATaaaa                                                                                          >  2:92131/1‑140 (MQ=255)                                              aaaGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTg                                                                                      >  1:263196/1‑140 (MQ=255)                                                  gCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTa                                                                                  >  1:9970/1‑140 (MQ=255)                                                   cGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTAt                                                                                 >  1:633686/1‑140 (MQ=255)                                                     tACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTAtct                                                                               >  1:553089/1‑140 (MQ=255)                                                     tACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTAtct                                                                               >  2:386265/1‑140 (MQ=255)                                                     tACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTAtct                                                                               >  1:24979/1‑140 (MQ=255)                                                       cAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTACAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTcg                                                                             <  1:526854/140‑1 (MQ=255)                                                         gTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTcgcg                                                                           <  1:386265/140‑1 (MQ=255)                                                         gTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTcgcg                                                                           <  2:454137/140‑1 (MQ=255)                                                            tCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTgg                                                                        <  2:633686/140‑1 (MQ=255)                                                            tCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTgg                                                                        >  1:1162552/1‑140 (MQ=255)                                                               cAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCgg                                                                     <  1:1082791/140‑1 (MQ=255)                                                               cAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCgg                                                                     <  1:234168/140‑1 (MQ=255)                                                                 aCCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGaa                                                                   <  2:24979/140‑1 (MQ=255)                                                                  ccATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAg                                                                  >  2:622961/1‑140 (MQ=255)                                                                         aTCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGa                                                           >  1:580653/1‑140 (MQ=255)                                                                            aTTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACAt                                                        >  1:470901/1‑140 (MQ=255)                                                                               ggCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTcc                                                     >  2:332352/1‑140 (MQ=255)                                                                                              cTGGCAAAATGCTACGCTGAAGAAAGCGCTGACGAAATGGTGCTATACGATATGACCGCTTCCAGCGATAGCAGTGAGGTAGATAAAAACTCGGTATCTCGCGTGGCCGAAGGGAGCGATATTCCGTTTTGTGTGGCgag                                      >  2:1207822/1‑138 (MQ=255)                                                                                              cTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGTggg                                      <  2:263196/140‑1 (MQ=255)                                                                                              cTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCggg                                      >  2:955974/1‑140 (MQ=255)                                                                                               tGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGt                                     <  2:982550/140‑1 (MQ=255)                                                                                               tGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGt                                     <  1:206071/140‑1 (MQ=255)                                                                                                ggCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTg                                    >  1:593850/1‑140 (MQ=255)                                                                                                     aaCGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGAtt                               <  2:1182619/140‑1 (MQ=255)                                                                                                      acgctacgctGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTTGGGTTa                              >  1:226016/1‑140 (MQ=255)                                                                                                                      ggCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTTAGTCTCTGGGAGATGc              >  2:719119/1‑140 (MQ=255)                                                                                                                      ggCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGGCTCTGGAAGATGc              >  2:546575/1‑140 (MQ=255)                                                                                                                      ggCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGATTAAGTCTCTGGAAGATGc              >  1:365874/1‑140 (MQ=255)                                                                                                                      ggCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGAGGCGGGTGGGATTAAGTCTCTGGAAGATGc              >  1:1019140/1‑140 (MQ=255)                                                                                                                                  cTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAATCTCTGGAAGATGCCGCGAAAAATCt  >  2:961548/1‑140 (MQ=255)                                                                                                                                  cTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCt  <  1:180530/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      AAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCT  >  NC_000913/2095853‑2096121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑