Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,141,905	A→G	V104A (GTG→GCG)	dcd ←	dCTP deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,141,905	0	A	G	100.0%	122.7 / NA	35	V104A (GTG→GCG)	dcd	dCTP deaminase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/15);  total (20/15)

GCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACTTC  >  NC_000913/2141771‑2142038                                                                                                                                       |                                                                                                                                      gcgcCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGa                                                                                                                                  >  2:834703/1‑140 (MQ=255)  cgcCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGATGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGAt                                                                                                                                 >  1:236191/1‑140 (MQ=255)                   cGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCAcc                                                                                                                <  2:954823/140‑1 (MQ=255)                     gAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCATATCGGCTGGCAGCGTCACCGa                                                                                                              >  1:530406/1‑140 (MQ=255)                             gAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCg                                                                                                      >  2:212325/1‑140 (MQ=255)                                     gCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgcc                                                                                              <  2:341817/140‑1 (MQ=255)                                     gCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgcc                                                                                              >  2:94767/1‑140 (MQ=255)                                     gCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgcc                                                                                              >  1:407288/1‑140 (MQ=255)                                           tGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgccagcgcc                                                                                        >  1:42039/1‑140 (MQ=255)                                            gCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgccagcgcca                                                                                       <  2:485707/140‑1 (MQ=255)                                             cAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgccagcgccag                                                                                      <  2:42039/140‑1 (MQ=255)                                               accagaccagCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGct                                                                                    <  1:94767/140‑1 (MQ=255)                                                ccagaccagCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGctc                                                                                   >  1:1005494/1‑140 (MQ=255)                                                   gaccagCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTcc                                                                                >  1:313552/1‑140 (MQ=255)                                                   gaccagCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTcc                                                                                >  2:738559/1‑140 (MQ=255)                                                   gaccagCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTcc                                                                                >  1:1107093/1‑140 (MQ=255)                                                     ccagCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTg                                                                              >  2:970301/1‑140 (MQ=255)                                                     ccagCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTg                                                                              >  1:1012082/1‑140 (MQ=255)                                                     ccagCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTg                                                                              >  1:606144/1‑140 (MQ=255)                                                                  aTGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGaa                                                                 <  1:212325/140‑1 (MQ=255)                                                                           gcgGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGcc                                                        <  1:145270/140‑1 (MQ=255)                                                                             ggTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCt                                                      >  1:557154/1‑140 (MQ=255)                                                                                      cACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGaa                                             <  2:557154/140‑1 (MQ=255)                                                                                       aCCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAAc                                            <  1:834703/140‑1 (MQ=255)                                                                                         cATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGTCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGa                                          >  1:358319/1‑140 (MQ=255)                                                                                          aTCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGAt                                         <  2:1107093/140‑1 (MQ=255)                                                                                            cAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGAtct                                       >  1:1042934/1‑140 (MQ=255)                                                                                                         gcCAGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCAc                          >  1:287255/1‑140 (MQ=255)                                                                                                            aGTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCAcgcg                       >  2:821972/1‑140 (MQ=255)                                                                                                             gTGAGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCgg                      >  1:79193/1‑140 (MQ=255)                                                                                                                           tCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCt        <  1:738559/140‑1 (MQ=255)                                                                                                                           tCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCt        <  1:970301/140‑1 (MQ=255)                                                                                                                           tCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCt        <  1:1063931/140‑1 (MQ=255)                                                                                                                               gccagccCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACt    <  2:1042934/140‑1 (MQ=255)                                                                                                                                 cagccCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACTTc  <  2:287255/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      GCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACTTC  >  NC_000913/2141771‑2142038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑