Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	468,271	(T)8→7	intergenic (+41/‑112)	cof → / → decR	HMP‑PP phosphatase/DNA‑binding transcriptional activator DecR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	468,264	0	T	.	100.0%	143.2 / NA	34	intergenic (+34/‑119)	cof/decR	HMP‑PP phosphatase/DNA‑binding transcriptional activator DecR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (21/13);  total (21/13)

CATTTACCGGTGATTGGACATTGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAAT  >  NC_000913/468135‑468396                                                                                                                                  |                                                                                                                                     cATTTACCGGTGATTGGACATTGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGGtttttttgcgt                                                                                                                            >  1:236630/1‑136 (MQ=255)             aTTGGACATTGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCAt                                                                                                               >  1:409648/1‑140 (MQ=255)              ttGGACATTGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCAtt                                                                                                              <  1:330218/140‑1 (MQ=255)                     ttGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGcc                                                                                                       <  1:245755/140‑1 (MQ=255)                      tGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCg                                                                                                      >  1:63305/1‑140 (MQ=255)                      tGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCg                                                                                                      >  2:40329/1‑140 (MQ=255)                       gCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCgt                                                                                                     >  2:310331/1‑140 (MQ=255)                                  gCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAAc                                                                                          >  2:351896/1‑140 (MQ=255)                                      tctctcACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGca                                                                                      <  1:351896/140‑1 (MQ=255)                                      tctctcACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGca                                                                                      <  2:63305/140‑1 (MQ=255)                                        tctcACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGcaca                                                                                    >  2:112271/1‑140 (MQ=255)                                                tttGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAAt                                                                            >  1:313980/1‑140 (MQ=255)                                                tttGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAAt                                                                            >  1:38604/1‑140 (MQ=255)                                                   gACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCTAGCACCGGGCAATTGCCCGG‑TTTTTTTACGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGt                                                                         >  2:451360/1‑140 (MQ=255)                                                        aCTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGa                                                                    >  2:223885/1‑140 (MQ=255)                                                                      ccACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTtaaaa                                                      >  2:38433/1‑140 (MQ=255)                                                                      ccACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGAGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTtaaaa                                                      >  1:30537/1‑140 (MQ=255)                                                                               ccTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTaa                                             >  1:138062/1‑140 (MQ=255)                                                                                  tATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAag                                          >  1:306074/1‑140 (MQ=255)                                                                                   aTTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAaga                                         >  2:146845/1‑140 (MQ=255)                                                                                   aTTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAaga                                         <  2:313980/140‑1 (MQ=255)                                                                                     tCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAgaga                                       <  1:310331/140‑1 (MQ=255)                                                                                         ccGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGaaaaa                                   >  1:325827/1‑140 (MQ=255)                                                                                          cGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGaaaaaa                                  >  1:147975/1‑140 (MQ=255)                                                                                                      tCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGa                      <  2:147975/140‑1 (MQ=255)                                                                                                       cccTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGaa                     >  2:86421/1‑140 (MQ=255)                                                                                                          ttCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAggg                  <  2:313165/140‑1 (MQ=255)                                                                                                               gCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATg             <  2:169633/140‑1 (MQ=255)                                                                                                               gCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATg             <  2:138062/140‑1 (MQ=255)                                                                                                               gCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATg             <  1:146845/140‑1 (MQ=255)                                                                                                                  ccGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGa                      <  2:112540/128‑1 (MQ=255)                                                                                                                  ccGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGa                      >  1:112540/1‑128 (MQ=255)                                                                                                                  ccGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTa          >  1:350440/1‑140 (MQ=255)                                                                                                                     ggCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGAt       >  2:284013/1‑140 (MQ=255)                                                                                                                          ttGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAAt  <  1:292532/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                     CATTTACCGGTGATTGGACATTGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAAT  >  NC_000913/468135‑468396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑