Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,162,501	Δ1 bp	coding (255/378 nt)	ycfL →	DUF1425 domain‑containing protein YcfL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,162,499	0	G	.	100.0%	125.7 / NA	30	coding (253/378 nt)	ycfL	DUF1425 domain‑containing protein YcfL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (13/17);  total (13/17)

TGCGGGCATCAGTGCAGAAAAGCCCTTCCTTTCGACGTCTGATATTCAACCTTCAGCATCCTCAACGCTTTATAACGAAAGGCAAGAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGAGGGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGGGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATC  >  NC_000913/1162369‑1162634                                                                                                                                   |                                                                                                                                        tGCGGGCATCAGTGCAGAAAAGCCCTTCCTTTCGACGTCTGATATTCAACCTTCAGCATCCTCAACGCTTTATAACGAAAGGCAAGAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGAt                                                                                                                               >  1:511174/1‑140 (MQ=255)   cGGGCATCAGTGCAGAAAAGCCCTTCCTTTCGACGTCTGATATTCAACCTTCAGCATCCTCAACGCTTTATAACGAAAGGCAAGAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGATGc                                                                                                                             <  2:253801/140‑1 (MQ=255)    gggCATCAGTGCAGAAAAGCCCTTCCTTTCGACGTCTGATATTCAACCTTCAGCATCCTCAACGCTTTATAACGAAAGGCAAGAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGATGc                                                                                                                             <  1:1552/139‑1 (MQ=255)    gggCATCAGTGCAGAAAAGCCCTTCCTTTCGACGTCTGATATTCAACCTTCAGCATCCTCAACGCTTTATAACGAAAGGCAAGAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGATGc                                                                                                                             >  2:1552/1‑139 (MQ=255)    gggCATCAGTGCAGAAAAGCCCTTCCTTTCGACGTCTGATATTCAACCTTCAGCATCCTCAACGCTTTATAACGAAAGGCAAGAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGATGCa                                                                                                                            <  1:496131/140‑1 (MQ=255)                aGAAAAGCCCTTCCTTTCGACGTCTGATATTCAACCTTCAGCATCCTCAACGCTTTATAACGAAAGGCAAGAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGATGCATCCTCTGGAAAg                                                                                                                <  1:446275/140‑1 (MQ=255)                    aaGCCCTTCCTTTCGACGTCTGATATTCAACCTTCAGCATCCTCAACGCTTTATAACGAAAGGCAAGAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGATGCATCCTCTGGAAAGGCCa                                                                                                            <  1:81947/140‑1 (MQ=255)                      gCCCTTCCTTTCGACGTCTGATATTCAACCTTCAGCATCCTCAACGCTTTATAACGAAAGGCAAGAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGATGCATCCTCTGGAAAGGCCACg                                                                                                          <  2:81143/140‑1 (MQ=255)                      gCCCTTCCTTTCGACGTCTGATATTCAACCTTCAGCATCCTCAACGCTTTATAACGAAAGGCAAGAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGATGCATCCTCTGGAAAGGCCACg                                                                                                          <  1:95461/140‑1 (MQ=255)                            ccttTCGACGTCTGATATTCAACCTTCAGCATCCTCAACGCTTTATAACGAAAGGCAAGAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGt                                                                                                    >  1:270460/1‑140 (MQ=255)                               ttCGACGTCTGATATTCAACCTTCAGCATCCTCAACGCTTTATAACGAAAGGCAAGAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTAc                                                                                                 <  2:270460/140‑1 (MQ=255)                                          atatTCAACCTTCAGCATCCTCAACGCTTTATAACGAAAGGCAAGAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCa                                                                                               <  1:516086/131‑1 (MQ=255)                                          atatTCAACCTTCAGCATCCTCAACGCTTTATAACGAAAGGCAAGAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCa                                                                                               >  2:516086/1‑131 (MQ=255)                                                aaCCTTCAGCATCCTCAACGCTTTATAACGAAAGGCAAGAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTcgg                                                                                >  2:328638/1‑140 (MQ=255)                                                  ccTTCAGCATCCTCAACGCTTTATAACGAAAGGCAAGAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTcggcg                                                                              >  2:489294/1‑140 (MQ=255)                                                                  cGCTTTATAACGAAAGGCAAGAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCa                                                              >  1:21781/1‑140 (MQ=255)                                                                       tataACGAAAGGCAAGAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGcc                                                         >  1:68613/1‑140 (MQ=255)                                                                               aaGGCAAGAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCt                                                 >  1:352431/1‑140 (MQ=255)                                                                                 ggCAAGAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTgg                                               >  2:398951/1‑140 (MQ=255)                                                                                   cAAGAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTgggg                                             <  2:237769/140‑1 (MQ=255)                                                                                   cAAGAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTgggg                                             <  2:352431/140‑1 (MQ=255)                                                                                       aaCCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGGgcgc                                         >  1:369974/1‑140 (MQ=255)                                                                                        aCCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGGGCGca                                        <  2:511174/140‑1 (MQ=255)                                                                                          ccGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGGGCGcaca                                      <  2:68613/140‑1 (MQ=255)                                                                                                   ttCATTATCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGGGCGCACAAAGTCAGAc                             <  2:369974/140‑1 (MQ=255)                                                                                                       ttATCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGGGCGCACAAAGTCAGACTTTa                         <  1:55458/140‑1 (MQ=255)                                                                                                        tATCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGGGCGCACAAAGTCAGACttt                          <  1:414451/138‑1 (MQ=255)                                                                                                        tATCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGGGCGCACAAAGTCAGACttt                          >  2:414451/1‑138 (MQ=255)                                                                                                         aTCGTTTTTACTGGTATGACGCCAGA‑GGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGGGCGCACAAAGTCAGACTTTATc                       <  1:7703/140‑1 (MQ=255)                                                                                                                              ccAGA‑GGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGGGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATc  >  2:234583/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                        TGCGGGCATCAGTGCAGAAAAGCCCTTCCTTTCGACGTCTGATATTCAACCTTCAGCATCCTCAACGCTTTATAACGAAAGGCAAGAACCCGTTACCGTTCATTATCGTTTTTACTGGTATGACGCCAGAGGGCTGGAGATGCATCCTCTGGAAAGGCCACGCAGCGTTACCATTCCCGCACATTCGGCGGTAACGCTGTACGGCAGCGCCAATTTTCTGGGGGCGCACAAAGTCAGACTTTATCTATATTTGTAAGGGGTGAATC  >  NC_000913/1162369‑1162634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑