Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,351,196	(G)7→6	intergenic (‑157/+211)	fabI ← / ← ycjD	enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,351,190	0	G	.	100.0%	116.4 / NA	28	intergenic (‑151/+217)	fabI/ycjD	enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (12/16);  total (12/16)

TGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGA  >  NC_000913/1351068‑1351319                                                                                                                           |                                                                                                                                  tgtgCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCt                                                                                                                 <  2:56678/140‑1 (MQ=255) tgtgCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCt                                                                                                                 <  2:282752/140‑1 (MQ=255)   tgCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCa                                                                                                               >  1:112054/1‑140 (MQ=255)     ccTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCAcc                                                                                                             >  2:21599/1‑140 (MQ=255)             tCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCct                                                                                                     <  2:112054/140‑1 (MQ=255)                     ttCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCag                                                                                             >  2:103718/1‑140 (MQ=255)                     ttCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCag                                                                                             >  2:538106/1‑140 (MQ=255)                      tcgtcgTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCGACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGGGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCaga                                                                                            <  1:440711/140‑1 (MQ=255)                             tttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                     <  1:538106/140‑1 (MQ=255)                             tttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                     <  1:21599/140‑1 (MQ=255)                                        gTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAt                                                                          <  2:77343/140‑1 (MQ=255)                                        gTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAt                                                                          <  2:35361/140‑1 (MQ=255)                                           cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgt                                                                       <  1:262771/140‑1 (MQ=255)                                                            attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCCAAGGGGCGGGGGGCCCGTATTGGGCAAATATTGTTAcccc                                                      >  1:168112/1‑140 (MQ=39)                                                                     aaCCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                             <  1:103718/140‑1 (MQ=255)                                                                     aaCCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                             <  1:35431/140‑1 (MQ=255)                                                                       ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                             <  1:346745/138‑1 (MQ=255)                                                                       ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                             >  2:346745/1‑138 (MQ=255)                                                                                                   gTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACa                                 <  1:71640/122‑1 (MQ=255)                                                                                                   gTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACa                                 >  2:71640/1‑122 (MQ=255)                                                                                                   gTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATg               >  2:517896/1‑140 (MQ=255)                                                                                                    tCCGACCGGAGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATgg              >  1:143342/1‑140 (MQ=255)                                                                                                             aGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCa                 <  1:314929/128‑1 (MQ=255)                                                                                                             aGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCa                 >  2:314929/1‑128 (MQ=255)                                                                                                             aGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTg     >  2:208480/1‑140 (MQ=255)                                                                                                             aGCAGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTg     >  2:365321/1‑140 (MQ=255)                                                                                                                aGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa  <  1:73560/140‑1 (MQ=255)                                                                                                                aGGCTGCGGCA‑GGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa  <  1:365028/140‑1 (MQ=255)                                                                                                                           |                                                                                                                                  TGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGA  >  NC_000913/1351068‑1351319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑