Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,891,751	(C)5→6	coding (1485/1911 nt)	yoaA ←	putative 5' to 3' DNA helicase implicated in DNA repair

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,891,746	1	.	C	100.0%	65.6 / NA	19	E497G (GAA→GGA)	yoaA	putative 5' to 3' DNA helicase implicated in DNA repair
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (9/10);  total (9/10)

AAACGGCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTT‑CCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACA  >  NC_000913/1891610‑1891825                                                                                                                                          |                                                                                aaaCGGCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTT‑ccc                                                                              <  2:160223/140‑1 (MQ=255)    cGGCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTT‑cccccc                                                                           <  2:244570/140‑2 (MQ=255)      gCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTT‑cccccctg                                                                         <  2:35321/140‑4 (MQ=255)            tGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGcaacaa                                                                    <  1:64589/140‑1 (MQ=255)                 aTAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAg                                                               >  1:518863/1‑140 (MQ=255)                   aaTTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCGTTGCTGGTTTCCCCCCTGCAACAATACAGGa                                                             <  2:411044/140‑1 (MQ=255)                     ttACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAg                                                           <  1:326826/140‑1 (MQ=255)                                 aTGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAgc                                               >  1:457765/1‑140 (MQ=255)                                       cGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGaa                                         >  2:170518/1‑140 (MQ=255)                                          cacgcacgTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTg                                      <  2:75687/140‑1 (MQ=255)                                            cgcacgTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCt                                    >  1:112686/1‑140 (MQ=255)                                                  tCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCa                              <  2:112686/140‑1 (MQ=255)                                                    cACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGa                            >  2:433729/1‑140 (MQ=255)                                                           tCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGca                     >  1:393176/1‑140 (MQ=255)                                                           tCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGca                     >  1:166894/1‑140 (MQ=255)                                                                  aaCTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGc              <  2:10353/140‑1 (MQ=255)                                                                       ctgGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCg         <  2:518863/140‑1 (MQ=255)                                                                            tGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTa    >  2:185651/1‑140 (MQ=255)                                                                              ccACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACa  <  1:185651/140‑1 (MQ=255)                                                                              ccACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACa  <  2:433139/140‑1 (MQ=255)                                                                               cacaAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGt            <  1:266391/129‑1 (MQ=255)                                                                               cacaAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGt            >  2:266391/1‑129 (MQ=255)                                                                                                                                          |                                                                                AAACGGCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTT‑CCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACA  >  NC_000913/1891610‑1891825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑