New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4373261 =NA (NA)20 (0.770) 10/226 1.2 NA intergenic (+99/+104) yjeI/yjeJ DUF4156 domain‑containing lipoprotein YjeI/protein YjeJ
?NC_000913 4374533 = NA (NA)intergenic (‑299/+96) yjeJ/epmB protein YjeJ/lysine 2,3‑aminomutase

AAGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTCAATGCACGATGTAGGCCTGATAAGCGTAGCGAATCAGGCAATTTTAATGTTTAACTTCCCTGTTTAATCAGTTACTGCTGGCGTAGCTGGAGATCCAGCGGCG  >  NC_000913/4374394‑4374660
                                                                                                                                           |                                                                                                                               
aatccaccagcaaaactgcaccacaggcgaaataatctcatctttgctaatgcgaggctggagccatccagcctgaaatgactgataacgtgatcgttggtcatgccggatgcagcgtaaacgccttatccggcctacag                                                                                                                                 >  2:500736‑M2/140‑140 (MQ=255)
aatccaccagcaaaactgcaccacaggcgaaataatctcatctttgctaatgcgaggctggagccatccagcctgaaatgactgataacgtgatcgttgctcttgccggatgcagcgtaaacgccttatccggcctacag                                                                                                                                 >  2:332898‑M2/140‑140 (MQ=255)
  tccaccagcaaaactgcaccacaggcgaaataatctcatctttgctaatgcgaggctggagccatccagcctgaaatgactgataacgtgatcgttggtcatgccggatgcggcgtaaacgccttatccggcctacaGtt                                                                                                                               <  2:124375‑M2/3‑1 (MQ=255)
  gTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGtt                                                                                                                               <  1:534809/140‑1 (MQ=255)
   ccaccagcaaaactgcgccacaggcgaaataatctcatctttgctaatgcgaggctggagccatccagcctgaaatgactgataacgtgatcgttggtcatgccggatgcggcgtaaacgccttatccggactacaGTTc                                                                                                                              <  2:311903‑M2/4‑1 (MQ=255)
   ccaccagcaaaactgcaccacaggcgaaataatctcatctttgctaatgcgaggctggagccatccagcctgaaatgactgataacgtgatcgttggtcatgccggatgcggcgtaaacgccttatccggcctacaGTTc                                                                                                                              <  1:506897‑M2/4‑1 (MQ=255)
   ccaccagcaaaactgcaccacaggcgaaataatctcatctttgctaatgcgaggctggagccatccagcctgaaatgactgataacgtgatcgttggtcatgccggatgcggcgtaaacgccttatccggcctacaGTTc                                                                                                                              <  1:469978‑M2/4‑1 (MQ=255)
    aacaacAGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCa                                                                                                                             >  2:264939/1‑140 (MQ=255)
      caacaGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCAtt                                                                                                                           >  2:414726/1‑140 (MQ=255)
       aacaGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTg                                                                                                                          <  2:303938/140‑1 (MQ=255)
            cAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAAt                                                                                                                     <  2:482481/140‑1 (MQ=255)
                    atGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCAttcaa                                                                                                             >  2:79232/1‑140 (MQ=255)
                    atGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCAttcaa                                                                                                             >  2:530380/1‑140 (MQ=255)
                    atGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCAttcaa                                                                                                             >  2:426065/1‑140 (MQ=255)
                      gAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCAttcaatt                                                                                                           >  2:110561/1‑140 (MQ=255)
                        aggcgaaataatctcatctttgctaatgcgaggctggagccatccagcctgaaatgactgataacgtgatcgttggtcatgccggatgcagcgtaaacgccttatccggcctacaGTTCATTGCAAATCCAttcaatttc                                                                                                         <  2:488774‑M2/25‑1 (MQ=255)
                         taatTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCAttcaatttca                                                                                                        <  1:293374/140‑1 (MQ=255)
                         taatTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCAAttcaatttca                                                                                                        <  2:352073/140‑1 (MQ=255)
                         taatTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCAAttcaatttca                                                                                                        >  1:352073/1‑140 (MQ=255)
                         ggcgaaataatctcatctttgctaatgcgaggctggagccatccagcctgaaatgactgataacgtgatcgttggtcatgccggatgcggcgtaaacgccttatccggcctacaGTTCATTGCAAATCCAttcaatttca                                                                                                        <  1:411506‑M2/26‑1 (MQ=255)
                         ggcgaaataatctcatctttgctaatgcgaggctggagccatccagcctgaaatgactgataacgtgatcgttggtcatgccggatgcggcgtaaacgccttatccggcctacaGTTCATTGCAAATCCAttcaatttca                                                                                                        <  1:352606‑M2/26‑1 (MQ=255)
                         ggcgaaataatctcatctttgctaatgcgaggctggagccatccagcctgaaatgactgataacgtgatcgttggtcatgccggatgcagcgtaaacgccttatccggcctacaGTTCATTGCAAATCCAttcaatttca                                                                                                        <  1:449707‑M2/26‑1 (MQ=255)
                         ggcgaaataatctcatctttgctaatgcgaggctggagccatccagcctgaaatgactgataacgtgatcgttggtcatgccggatgcagcgtaaacgccttatccggcctacaGTTCATTGCAAATCCAttcaatttca                                                                                                        <  1:500736‑M2/26‑1 (MQ=255)
                         ggcgaaataatctcatctttgctaatgcgaggctggagccatccagcctgaaatgactgataacgtgatcgttgctcttgccggatgcagcgtaaacgccttatccggcctacaGTTCATTGCAAATCCAttcaatttca                                                                                                        <  1:332898‑M2/26‑1 (MQ=255)
                           atTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCAttcaatttcaat                                                                                                      >  2:422163/1‑140 (MQ=255)
                           atTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCAttcaatttcaat                                                                                                      >  1:220396/1‑140 (MQ=255)
                            ttAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTCAATg                                                                                                     >  1:166666/1‑140 (MQ=255)
                              aataatctcatctttgctaatgcgaggctggagccatccagcctgaaatgactgataacgtgatcgttggtcatgccggatgcagcgtaaacgccttatccggcctacaGTTCATTGCAAATCCAttcaatttca                                                                                                        <  1:354445‑M2/26‑1 (MQ=255)
                              aataatctcatctttgctaatgcgaggctggagccatccagcctgaaatgactgataacgtgatcgttggtcatgccggatgcagcgtaaacgccttatccggcctacaGTTCATTGCAAATCCAttcaatttca                                                                                                        >  2:354445‑M2/110‑135 (MQ=255)
                              aaaGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTCAATGCa                                                                                                   <  2:193389/140‑1 (MQ=255)
                              aaaGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTCAATGCa                                                                                                   <  2:206019/140‑1 (MQ=255)
                              aaaGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTCAATGCa                                                                                                   <  1:197763/140‑1 (MQ=255)
                              aaaGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTCAATGCa                                                                                                   <  1:153053/140‑1 (MQ=255)
                              aaaGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTCAATGCa                                                                                                   <  2:62132/140‑1 (MQ=255)
                                 aatctcatctttgctaatgcgaggctggagccatgcagcctgaaatgactgataacgtgatcgttggtcatgccggatgcggcgtaaacgccttatccggcctacaGTTCATTGCAAATCCAttcaatttca                                                                                                        >  2:479043‑M2/107‑132 (MQ=255)
                                 aatctcatctttgctaatgcgaggctggagccatccagcctgaaatgactgataacgtgatcgttggtcatgccggatgcggcgtaaacgccttatccggcctacaGTTCATTGCAAATCCAttcaatttca                                                                                                        <  1:479043‑M2/26‑1 (MQ=255)
                                   ttATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTCAATGCACGATg                                                                                              <  2:116125/140‑1 (MQ=255)
                                   ttATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTCAATGCACGATg                                                                                              <  1:422163/140‑1 (MQ=255)
                                     aTGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTTAATGCACGATGTa                                                                                            >  2:423799/1‑140 (MQ=255)
                                     aTGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTCAATGCACGATGTa                                                                                            <  1:79232/140‑1 (MQ=255)
                                        tctttgctaatgcgaggctggagccatccagcctgaaatgactgataacgtgatcgttggtcatgccggatgcggcgtaaacgccttatccggcctacaGTTCATTGCAAATCCAttcaatttca                                                                                                        <  2:491220‑M2/26‑1 (MQ=255)
                                        tctttgctaatgcgaggctggagccatccagcctgaaatgactgataacgtgatcgttggtcatgccggatgcggcgtaaacgccttatccggcctacaGTTCATTGCAAATCCAttcaatttca                                                                                                        >  1:491220‑M2/100‑125 (MQ=255)
                                           cGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTCAATGCACGATGTAGGCCTg                                                                                      >  1:156171/1‑140 (MQ=255)
                                            gggCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTCAATGCACGATGTAGGCCTGa                                                                                     >  2:431727/1‑140 (MQ=255)
                                              gCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTCAATGCACGATGTAGGCCTGATa                                                                                   <  2:76870/140‑1 (MQ=255)
                                                aaaGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTCAATGCACGATGTAGGCCTGATAAg                                                                                 <  2:191903/140‑1 (MQ=255)
                                                aaaGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTCAATGCACGATGTAGGCCTGATAAg                                                                                 <  1:480495/140‑1 (MQ=255)
                                                              ccAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTCAATGCACGATGTAGGCCTGATAAGCGTAGCGAATCAgg                                                                   <  1:271285/140‑1 (MQ=255)
                                                              ccAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTCAATGCACGATGTAGGCCTGATAAGCGTAGCGAATCAgg                                                                   <  2:438036/140‑1 (MQ=255)
                                                                      gcctgaaatgactgataacgtgatcgttggtcatgccggatgcggcgtaaacgccttatccggcctacaGTTCATTGCAAATCCATTCAATTTCAATGCACGATGTAGGCCTGATAAGCGTAGCGAATCAGGCAATTTTa                                                           >  2:123832‑M2/70‑140 (MQ=255)
                                                                         aCAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTTAATGCACGATGTAGGCCTGATAAGCGTAGCGAATCAGGCAATTTTAATg                                                        <  1:423799/140‑1 (MQ=255)
                                                                            ggTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTCAATGCACGATGTAGGCCTGATAAGCGTAGCGAATCAGGCAATTTTAATGttt                                                     >  1:435266/1‑140 (MQ=255)
                                                                                           gTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTCAATGCACGATGTAGGCCTGATAAGCGTAGCGAATCAGGCAATTTTAATGTTTAACTTCCCTGTTTaa                                      <  2:62721/140‑1 (MQ=255)
                                                                                              tgttgCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTCAATGCACGATGTAGGCCTGATAAGCGTAGCGAATCAGGCAATTTTAATGTTTAACTTCCCTGtt                                         <  1:116965/134‑1 (MQ=255)
                                                                                              tgttgCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTCAATGCACGATGTAGGCCTGATAAGCGTAGCGAATCAGGCAATTTTAATGTTTAACTTCCCTGtt                                         >  2:116965/1‑134 (MQ=255)
                                                                                                   ctctTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTCAATGCACGATGTAGGCCTGATAAGCGTAGCGAATCAGGCAATTTTAATGTTTAACTTCCCTGTTTaa                                      <  1:388003/132‑1 (MQ=255)
                                                                                                   ctctTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTCAATGCACGATGTAGGCCTGATAAGCGTAGCGAATCAGGCAATTTTAATGTTTAACTTCCCTGTTTaa                                      >  2:388003/1‑132 (MQ=255)
                                                                                                         ccGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTCAATGCACGATGTAGGCCTGATAAGCGTAGCGAATCAGGCAATTTTAATGTTTAACTTCCCTGTTTAATCAGTTACTGCTgg                        <  1:17421/140‑1 (MQ=255)
                                                                                                           ggATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTCAATGCACGATGTAGGCCTGATAAGCGTAGCGAATCAGGCAATTTTAATGTTTAACTTCCCTGTTTAATCAGTTACTGCTGGCg                      <  2:166666/140‑1 (MQ=255)
                                                                                                             aTGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTCAATGCACGATGTAGGCCTGATAAGCGTAGCGAATCAGGCAATTTTAATGTTTAACTTCCCTGTTTAATCAGTTACTGCTGGCGTa                    <  1:31714/140‑1 (MQ=255)
                                                                                                               gcggcgtaaacgccttatccggcctacaGTTCATTGCAAATCCATTCAATTTCAATGCACGATGTAGGCCTGATAAGCGTAGCGAATCAGGCAATTTTAATGTTTAACTTCCCTGTTTAATCAGTTACTGCTGGCGTAGc                  <  1:123832‑M2/112‑1 (MQ=255)
                                                                                                                cAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTCAATGCACGATGTAGGCCTGATAAGCGTAGCGAATCAGGCAATTTTAATGTTTAACTTCCCTGTTTAATCAGTTACTGCTGGCGTAGCt                 >  1:63924/1‑140 (MQ=255)
                                                                                                                   cgtaaacgccttatccggcctacaGTTCATTGCAAATCCATTCAATTTCAATGCACGATGTAGGCCTGATAAGCGTAGCGAATCAGGCAATTTTAATGTTTAACTTCCCTGTTTAATCAGTTActg                            <  2:474583‑M2/102‑1 (MQ=255)
                                                                                                                   cgtaaacgccttatccggcctacaGTTCATTGCAAATCCATTCAATTTCAATGCACGATGTAGGCCTGATAAGCGTAGCGAATCAGGCAATTTTAATGTTTAACTTCCCTGTTTAATCAGTTActg                            >  1:474583‑M2/25‑126 (MQ=255)
                                                                                                                               atccggcctacaGTTCATTGCAAATCCATTCAATTTCAATGCACGATGTAGGCCTGATAAGCGTAGCGAATCAGGCAATTTTAATGTTTAACTTCCCTGTTTAATCAGTTACTGCTGGCGTAGCTGGAGATCCAgcggcg  >  2:91067‑M2/13‑140 (MQ=255)
                                                                                                                                           |                                                                                                                               
AAGTAACAACAGCAATGCATATGAATAATTAAAGGTTATGCAACGGGCAAAGATTAAACTTCCCAGTTTAATGACAGGTTGCTGTACTTGCGTTTGTTGCTCTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAGTTCATTGCAAATCCATTCAATTTCAATGCACGATGTAGGCCTGATAAGCGTAGCGAATCAGGCAATTTTAATGTTTAACTTCCCTGTTTAATCAGTTACTGCTGGCGTAGCTGGAGATCCAGCGGCG  >  NC_000913/4374394‑4374660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.