Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	17,621	C→T	P45S (CCG→TCG)	nhaA →	Na(+):H(+) antiporter NhaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	17,621	0	C	T	100.0%	190.8 / NA	55	P45S (CCG→TCG)	nhaA	Na(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/30);  total (25/30)

AAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGC  >  NC_000913/17487‑17754                                                                                                                                       |                                                                                                                                      aaGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGtt                                                                                                                                  <  2:155094/140‑1 (MQ=255)           tCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCggg                                                                                                                        <  2:216807/140‑1 (MQ=255)                 tCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGggttggtt                                                                                                                  <  2:110203/140‑1 (MQ=255)                    aTTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCAc                                                                                                               >  1:529170/1‑140 (MQ=255)                                 gATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCA‑‑‑‑‑‑‑GTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATg                                                                                           <  1:158776/140‑1 (MQ=255)                                  aTGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACaaa                                                                                                 >  1:121256/1‑140 (MQ=255)                                  aTGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACaaa                                                                                                 >  2:178526/1‑140 (MQ=255)                                   tGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACaaaa                                                                                                <  2:514840/140‑1 (MQ=255)                                             ggCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAATAGCGGCGCAACCAGTGGATGGTATCTCGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGtt                                                                                      <  1:123683/140‑1 (MQ=255)                                             ggCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGtt                                                                                      <  1:299462/140‑1 (MQ=255)                                             ggCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTACGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGtt                                                                                      <  1:435800/140‑1 (MQ=255)                                              gCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTa                                                                                     <  1:470129/140‑1 (MQ=255)                                                 ttattCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATg                                                                                           >  2:355171/1‑131 (MQ=255)                                                 ttattCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATg                                                                                           <  1:355171/131‑1 (MQ=255)                                                      cTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATaaa                                                                             <  2:483503/140‑1 (MQ=255)                                                            aTTGCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGAcgc                                                                       >  1:438457/1‑140 (MQ=255)                                                              tGCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACgcgc                                                                     >  1:145931/1‑140 (MQ=255)                                                              tGCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACgcgc                                                                     >  1:283743/1‑140 (MQ=255)                                                               gCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCt                                                                    >  1:540160/1‑140 (MQ=255)                                                               gCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCt                                                                    >  1:452735/1‑140 (MQ=255)                                                                    tATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATgg                                                               <  2:2415/140‑1 (MQ=255)                                                                      tCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCg                                                             >  1:145005/1‑140 (MQ=255)                                                                           gCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTAtt                                                        >  1:168826/1‑140 (MQ=255)                                                                            cGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCgg                                                            <  1:302581/135‑1 (MQ=255)                                                                            cGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCgg                                                            >  2:302581/1‑135 (MQ=255)                                                                               tgatTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTc                                                    <  2:380295/140‑1 (MQ=255)                                                                                gatTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTAttttttc                                                   >  1:523730/1‑138 (MQ=255)                                                                                gatTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTcc                                                   >  2:362009/1‑140 (MQ=255)                                                                                 atTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCt                                                  >  1:340530/1‑140 (MQ=255)                                                                                      ggCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAg                                             <  1:362009/140‑1 (MQ=255)                                                                                      ggCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAg                                             <  2:540160/140‑1 (MQ=255)                                                                                           aCAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGt                                        >  1:58373/1‑140 (MQ=255)                                                                                              gcggcgCAACCAGTGGATGGTATCACGACTTTCTGGAGCCGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTg                                     <  2:452735/140‑1 (MQ=255)                                                                                              gcggcgCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTg                                     <  2:168826/140‑1 (MQ=255)                                                                                               cggcgCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTgg                                    >  1:263134/1‑140 (MQ=255)                                                                                               cggcgCAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTgg                                    >  2:536890/1‑140 (MQ=255)                                                                                                  cgcAACCAGTGGATGGTATCACGACTTTCTGGAGCCGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGCATTTTTCCTGTTAGTCGGTCTGGAAg                                 >  2:154264/1‑140 (MQ=255)                                                                                                   gcAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGt                                <  2:58373/140‑1 (MQ=255)                                                                                                   gcAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGt                                <  2:145931/140‑1 (MQ=255)                                                                                                   gcAACCAGTGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGt                                <  1:15763/140‑1 (MQ=255)                                                                                                           tGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTg                        <  1:178526/140‑1 (MQ=255)                                                                                                           tGGATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTg                        <  1:154264/140‑1 (MQ=255)                                                                                                             gATGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGaa                      <  2:246842/140‑1 (MQ=255)                                                                                                               tGGTATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACt                    <  2:263134/140‑1 (MQ=255)                                                                                                                  tATCACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGAt                 >  1:236000/1‑140 (MQ=255)                                                                                                                     cACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTg                        <  2:68769/130‑1 (MQ=255)                                                                                                                     cACGACTTTCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTg                        >  1:68769/1‑130 (MQ=255)                                                                                                                           tttCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAAc                     >  2:121867/1‑127 (MQ=255)                                                                                                                           tttCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAAc                     <  1:121867/127‑1 (MQ=255)                                                                                                                            ttCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCg       >  2:520346/1‑140 (MQ=255)                                                                                                                            ttCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCg       >  1:370049/1‑140 (MQ=255)                                                                                                                             tCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGc      >  1:226033/1‑140 (MQ=255)                                                                                                                             tCTGGAGACGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTAAACTGATGCAAGGATCGc      >  1:332280/1‑140 (MQ=255)                                                                                                                                 gagaCGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGc  <  2:370049/140‑1 (MQ=255)                                                                                                                                 gagaCGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGc  <  2:279737/140‑1 (MQ=255)                                                                                                                                 gagaCGTCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTAAACTGATGCAAGGATCGCTAGc  <  2:332280/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      AAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGC  >  NC_000913/17487‑17754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑