Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	211,504	C→T	A609V (GCA→GTA)	ldcC →	lysine decarboxylase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	211,504	0	C	T	100.0%	130.4 / NA	40	A609V (GCA→GTA)	ldcC	lysine decarboxylase 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/22);  total (18/22)

GATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCAC  >  NC_000913/211373‑211638                                                                                                                                    |                                                                                                                                       gATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATa                                                                                                                                >  1:306422/1‑140 (MQ=255)         atatGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCg                                                                                                                        <  1:469722/140‑1 (MQ=255)           atGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGga                                                                                                                      <  2:70889/140‑1 (MQ=255)                   cGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCa                                                                                                              <  1:369261/140‑1 (MQ=255)                      tctcTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGa                                                                                                           >  2:211832/1‑140 (MQ=255)                          tATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGcc                                                                                                       <  1:47884/140‑1 (MQ=255)                           aTGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACg                                                                                                         <  1:403637/137‑1 (MQ=255)                           aTGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACg                                                                                                         >  2:403637/1‑137 (MQ=255)                            tGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCcac                                                                                                     >  1:275766/1‑140 (MQ=255)                              cAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCCACAt                                                                                                   <  2:358073/140‑1 (MQ=255)                                  agaTCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAgg                                                                                               <  1:490137/140‑1 (MQ=255)                                    aTCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCa                                                                                             >  2:476505/1‑140 (MQ=255)                                    aTCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCa                                                                                             >  1:274050/1‑140 (MQ=255)                                      cccGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATg                                                                                           >  1:197321/1‑140 (MQ=255)                                      cccGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCACGACGCCACATCAGGCATg                                                                                           >  1:151419/1‑140 (MQ=255)                                                         tGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGc                                                                        >  2:437261/1‑140 (MQ=255)                                                         tGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGc                                                                        <  2:66912/140‑1 (MQ=255)                                                          gCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCg                                                                       <  1:256078/140‑1 (MQ=255)                                                          gCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCg                                                                       <  1:211832/140‑1 (MQ=255)                                                          gCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCg                                                                       <  1:64492/140‑1 (MQ=255)                                                          gCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCACGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCg                                                                       <  2:151419/140‑1 (MQ=255)                                                                   ggATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGaaa                                                              >  2:152833/1‑140 (MQ=255)                                                                      tCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCa                                                           <  1:414136/140‑1 (MQ=255)                                                                      tCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCa                                                           <  2:274050/140‑1 (MQ=255)                                                                          gCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTgc                                                       >  1:214869/1‑140 (MQ=255)                                                                                gggATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGa                                                 >  1:126960/1‑140 (MQ=255)                                                                                gggATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGa                                                 >  1:518974/1‑140 (MQ=255)                                                                                     ccATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGaacaac                                            >  1:41807/1‑140 (MQ=255)                                                                                        tAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTgg                                         >  2:456503/1‑140 (MQ=255)                                                                                         aaGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGt                                        <  1:112305/140‑1 (MQ=255)                                                                                         aaGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGt                                        <  1:271882/140‑1 (MQ=255)                                                                                               aTTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTag                                  >  1:173157/1‑140 (MQ=255)                                                                                                   gTAAACACGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTa                              <  2:306422/140‑1 (MQ=255)                                                                                                       acacGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCgg                          <  2:126960/140‑1 (MQ=255)                                                                                                        cacGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGc                         <  1:395365/140‑1 (MQ=255)                                                                                                         acGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCCACATCGGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCgg                          <  1:420612/138‑1 (MQ=255)                                                                                                         acGATCTTCCCGGTTTGATGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCCACATCGGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCgg                          >  2:420612/1‑138 (MQ=255)                                                                                                                           tGTTGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTAt      >  2:72924/1‑140 (MQ=255)                                                                                                                             ttGCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATcc    <  2:197321/140‑1 (MQ=255)                                                                                                                               gCGGGTATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCAc  <  2:103014/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                       GATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCAC  >  NC_000913/211373‑211638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑