Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,394,574	T→C	Y158C (TAC→TGC)	nuoL ←	NADH:quinone oxidoreductase subunit L

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,394,574	0	T	C	100.0%	84.5 / NA	24	Y158C (TAC→TGC)	nuoL	NADH:quinone oxidoreductase subunit L
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/10);  total (14/10)

CACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGTAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGC  >  NC_000913/2394444‑2394703                                                                                                                                   |                                                                                                                                  caccaTTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAAcc                                                                                                                          >  2:224255/1‑140 (MQ=255)     aTTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGAt                                                                                                                      <  2:442954/140‑1 (MQ=255)       ttCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATca                                                                                                                    >  1:173615/1‑140 (MQ=255)             gAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGAt                                                                                                              <  1:224255/140‑1 (MQ=255)                     gggTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGcac                                                                                                      <  2:284656/140‑1 (MQ=255)                        tGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAgg                                                                                                   >  2:76121/1‑140 (MQ=255)                                    tGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTcc                                                                                       >  1:273966/1‑140 (MQ=255)                                             tGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAgg                                                                              <  2:412505/140‑1 (MQ=255)                                                      cGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAg                                                                                    >  1:37734/1‑125 (MQ=255)                                                      cGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAg                                                                                    <  2:37734/125‑1 (MQ=255)                                                              aGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGtt                                                             >  1:433414/1‑140 (MQ=255)                                                              aGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGtt                                                             >  2:175567/1‑140 (MQ=255)                                                              aGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGtt                                                             >  2:537537/1‑140 (MQ=255)                                                              aGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGtt                                                             >  2:402730/1‑140 (MQ=255)                                                                              cacGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGa                                             >  1:194246/1‑140 (MQ=255)                                                                                  ggTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAacca                                         <  1:537537/140‑1 (MQ=255)                                                                                  ggTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAacca                                         >  1:66308/1‑140 (MQ=255)                                                                                      acgacgAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCAt                                     >  1:483899/1‑140 (MQ=255)                                                                                             aCGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCg                              >  1:101197/1‑140 (MQ=255)                                                                                             aCGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCg                              <  2:29842/140‑1 (MQ=255)                                                                                                      ttGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAgg                     <  1:76121/140‑1 (MQ=255)                                                                                                         ccGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTg                  >  2:234003/1‑140 (MQ=255)                                                                                                                   aTTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCga        <  2:66308/140‑1 (MQ=255)                                                                                                                         cGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGc  <  1:234003/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                  CACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGTAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGC  >  NC_000913/2394444‑2394703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑