Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,407,664	T→C	V35A (GTA→GCA)	alaA →	glutamate‑‑pyruvate aminotransferase AlaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,407,664	0	T	C	100.0%	92.8 / NA	26	V35A (GTA→GCA)	alaA	glutamate‑‑pyruvate aminotransferase AlaA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/14);  total (12/14)

GCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAG  >  NC_000913/2407533‑2407798                                                                                                                                    |                                                                                                                                       gCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGCactgaaac                                                                                                                                >  1:378776/1‑140 (MQ=255)           cAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGCACTGAAACTGAACATCgg                                                                                                                      <  1:13526/140‑1 (MQ=255)           cAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGCACTGAAACTGAACATCgg                                                                                                                      <  2:378776/140‑1 (MQ=255)           cAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGCACTGAAACTGAACATCgg                                                                                                                      <  2:360594/140‑1 (MQ=255)           cAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGCACTGAAACTGAACATCgg                                                                                                                      <  2:144495/140‑1 (MQ=255)                    tCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTTACAAGGCACTGAAACTGAACATCGGCAACCCAGc                                                                                                             <  1:94835/140‑1 (MQ=255)                             aTGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGCACTGAAACTGAACATCGGCAACCCAGCCCCGTTCgg                                                                                                    >  2:158723/1‑140 (MQ=255)                                   cccATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGCACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGa                                                                                              >  2:389450/1‑140 (MQ=255)                                       ttGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGCACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGAcgcg                                                                                          >  1:507912/1‑140 (MQ=255)                                        tGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGCACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACgcgc                                                                                         <  1:413844/140‑1 (MQ=255)                                                    cAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGCACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATcc                                                                             >  1:147161/1‑140 (MQ=255)                                                       ataagagAATGTCTGTTATGACACCCGTGGTCCGGTGCTAAAAGAAGCAAAACGCC‑GGAAGAAGAAGGTAACAAGGCACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGt                                                                         <  1:167745/137‑1 (MQ=255)                                                                   cTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGCACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATAc                                                              <  2:147161/140‑1 (MQ=255)                                                                     gTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGCACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGc                                                            <  2:85305/140‑1 (MQ=255)                                                                        aTGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGCACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAAc                                                         >  2:381617/1‑140 (MQ=255)                                                                                          tGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGCACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAggg                                       <  2:66525/140‑1 (MQ=255)                                                                                                  gaagCAAAACGCCTGGAAGAAGAAGGTAACAAGGCACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGa                               <  1:495521/140‑1 (MQ=255)                                                                                                    agCAAAACGCCTGGAAGAAGAAGGTAACAAGGCACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGAtt                             >  2:439165/1‑140 (MQ=255)                                                                                                      cAAAACGCCTGGAAGAAGAAGGTAACAAGGCACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTcc                           >  1:218128/1‑140 (MQ=255)                                                                                                       aaaaCGCCTGGAAGAAGAAGGTAACAAGGCACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCa                          <  2:218128/140‑1 (MQ=255)                                                                                                       aaaaCGCCTGGAAGAAGAAGGTAACAAGGCACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCa                          <  1:159015/140‑1 (MQ=255)                                                                                                             ccTGGAAGAAGAAGGAAACAAGGCACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTc                    >  2:181988/1‑140 (MQ=255)                                                                                                                      agaagGTAACAAGGCACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGAGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCcg           >  1:292414/1‑140 (MQ=255)                                                                                                                        aagGTAACAAGGCACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCcgcg         <  1:436520/140‑1 (MQ=255)                                                                                                                           gTAACAAGGCACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGt      >  1:434484/1‑140 (MQ=255)                                                                                                                               cAAGGCACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAg  >  2:348715/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                       GCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAG  >  NC_000913/2407533‑2407798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑