Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,431,321	G→A	P122S (CCA→TCA)	dedD ←	cell division protein DedD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,431,321	0	G	A	100.0%	48.7 / NA	16	P122S (CCA→TCA)	dedD	cell division protein DedD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/9);  total (7/9)

ACCGCGCAGCTTACCGACAATCTCATTCACTTTATCGGCATTTTTCAGCGCACCCAGTTGCACAACATAGGCTTTACCCGTCGGTGCAGCTTTTTCTTCCACGACCGGCTTCGGTTCTGGCTTCGGCGCAGGTGGTGCTTCAACCTTTGGTTTAGGCGGCTCCACCGGTTTCGGCTTCGGTGGGGCGACCGGTGCAGGTTCCGGTTCAAACTCGGTGTTATTGGCTGCAATAGTGGCCGGATCGAGCGACGGTGCTGCGGC  >  NC_000913/2431187‑2431447                                                                                                                                       |                                                                                                                               aCCGCGCAGCTTACCGACAATCTCATTCACTTTATCGGCATTTTTCAGCGCACCCAGTTGCACAACATAGGCTTTACCCGTCGGTGCAGCTTTTTCTTCCACGACCGGCTTCGGTTCTGGCTTCGGCGCAGGTGATGCtt                                                                                                                           <  2:335999/140‑1 (MQ=255)                           tCACTTTATCGGCATTTTTCAGCGCACCCAGTTGCACAACATAGGCTTTACCCGTCGGTGCAGCTTTTTCTTCCACGACCGGCTTCGGTTCTGGCTTCGGCGCAGGTGATGCTTCAACCTTTGGTTTAGGCGGCTCCAcc                                                                                                 <  2:444354/140‑1 (MQ=255)                               tttATCGGCATTTTTCAGCGCACCCAGTTGCACAACATAGGCTTTACCCGTCGGTGCAGCTTTTTCTTCCACGACCGGCTTCGGTTCTGGCTTCGGCGCAGGTGATGCTTCAACCTTTGGTTTAGGCGGCTCCACCGGtt                                                                                             >  1:88666/1‑140 (MQ=255)                                 tATCGGCATTTTTCAGCGCACCCAGTTGCACAACATAGGCTTTACCCGTCGGTGCAGCTTTTTCTTCCACGACCGGCTTCGGTTCTGGCTTCGGCGCAGGTGATGCTTCAACCTTTGGTTTAGGCGGCTCCACCGGTTTc                                                                                           >  1:409596/1‑140 (MQ=255)                                                                         tttACCCGTCGGTGCAGCTTTTTCTTCCACGACCGGCTTCGGTTCTGGCTTCGGCGCAGGTGATGCTTCAACCTTTGGTTTAGGCGGCTCCACCGGTTTCGGCTTCGGTGGGGCGACCGGTGCAGGTTCCGGTTCAAACt                                                   <  2:409596/140‑1 (MQ=255)                                                                            aCCGGTCGGTGCAGGTTTTTCTTCCGCGACCGGCTTCGGTTCTGGCTTCGGCGCAGGTGATGCTTCAACCTTTGGTTTAGGCGGCTCCACCGGTTTCGGCTTCGGTGGGGCGACCGGTGCAGGTTCCGGTTCAAACTCgg                                                <  2:508488/140‑1 (MQ=255)                                                                            aCCCGTCGGTGCAGCTTTTTCTTCCACGACCGGCTTCGGTTCTGGCTTCGGCGCAGGTGATGCTTCAACCTTTGGTTTAGGCGGCTCCACCGGTTTCGGCTTCGGTGGGGCGACCGGTGCAGGTTCCGGTTCAAAcgtgt                                                >  1:349428/1‑136 (MQ=255)                                                                                 tCGGTGCAGCTTTTTCTTCCACGACCGGCTTCGGTTCTGGCTTCGGCGCAGGTGATGCTTCAACCTTTGGTTTAGGCGGCTCCACCGGTTTCGGCTTCGGTGGGGCGACCGGTGCAGGTTCCGGTTCAGACTCGGTGTTa                                           >  1:158793/1‑140 (MQ=255)                                                                                 tCGGTGCAGCTTTTTCTTCCACGACCGGCTTCGGTTCTGGCTTCGGCGCAGGTGATGCTTCAACCTTTGGTTTAGGCGGCTCCACCGGTTTCGGCTTCGGTGGGGCGACCGGTGCAGGTTCCGGTTCAAACTCGGTGTTa                                           >  1:446243/1‑140 (MQ=255)                                                                                   ggTGCAGCTTTTTCTTCCACGACCGGCTTCGGTTCTGGCTTCGGCGCAGGTGATGCTTCAACCTTTGGTTTAGGCGGCTCCACCGGTTTCGGCTTCGGTGGGGCGACCGGTGCAGGTTCCGGTTCAAACTCGGTGTTAtt                                         <  1:125744/140‑1 (MQ=255)                                                                                        aGCTTTTTCTTCCACGACCGGCTTCGGTTCTGGCTTCGGCGCAGGTGATGCTTCAACCTTTGGTTTAGGCGGCTCCACCGGTTTCGGCTTCGGTGGGGCGACCGGTGCAGGTTCCGGTTCAAACTCGGTGTTATTGGCTg                                    <  2:88666/140‑1 (MQ=255)                                                                                                    cACGACCGGCTTCGGTTCTGGCTTCGGCGCAGGTGATGCTTCAACCTTTGGTTTAGGCGGCTCCACCGGTTTCGGCTTCGGTGGGGCGACCGGTGCAGGTTCCGGTTCAAACTCGGTGTTATTGGCTGCAATAGTGGCCg                        >  2:502866/1‑140 (MQ=255)                                                                                                       gACCGGCTTCGGTTCTGGCTTCGGCGCAGGTGATGCTTCAACCTTTGGTTTAGGCGGCTCCACCGGTTTCGGCTTCGGTGGGGCGACCGGTGCAGGTTCCGGTTCAAACTCGGTGTTATTGGCTGCAATAGTGGCCGGAt                     <  1:502866/140‑1 (MQ=255)                                                                                                             cTTCGGTTCTGGCTTCGGCGCAGGTGATGCTTCAACCTTTGGTTTAGGCGGCTCCACCGGTTTCGGCTTCGGTGGGGCGACCGGTGCAGGTTCCGGTTCAAACTCGGTGTTATTGGCTGCAATAGTGGCCGGATCGAGCg               >  1:26257/1‑140 (MQ=255)                                                                                                              ttCGGTTCTGGCTTCGGCGCAGGTGATGCTTCAACCTTTGGTTTAGGCGGCTCCACCGGTTTCGGCTTCGGTGGGGCGACCGGTGCAGGTTCCGGTTCAAACTCGGTGTTATTGGCTGCAATAGTGGCCGGATCGAGCGa              <  2:385236/140‑1 (MQ=255)                                                                                                                          ttCGGCGCAGGTGATGCTTCAACCTTTGGTTTAGGCGGCTCCACCGGTTTCGGCTTCGGTGGGGCGACCGGTGCAGGTTCCGGTTCAAACTCGGTGTTATTGGCTGCAATAGTGGCCGGATCGAGCGACGGTGCTGCGGc  <  2:37109/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                               ACCGCGCAGCTTACCGACAATCTCATTCACTTTATCGGCATTTTTCAGCGCACCCAGTTGCACAACATAGGCTTTACCCGTCGGTGCAGCTTTTTCTTCCACGACCGGCTTCGGTTCTGGCTTCGGCGCAGGTGGTGCTTCAACCTTTGGTTTAGGCGGCTCCACCGGTTTCGGCTTCGGTGGGGCGACCGGTGCAGGTTCCGGTTCAAACTCGGTGTTATTGGCTGCAATAGTGGCCGGATCGAGCGACGGTGCTGCGGC  >  NC_000913/2431187‑2431447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑