Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,465,426	G→A	E42E (GAG→GAA)	yfdC →	inner membrane protein YfdC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,465,426	0	G	A	100.0%	84.3 / NA	25	E42E (GAG→GAA)	yfdC	inner membrane protein YfdC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/15);  total (10/15)

GAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGA  >  NC_000913/2465295‑2465554                                                                                                                                    |                                                                                                                                 gagaCCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCg                                                                                                                          >  2:401015/1‑140 (MQ=255)         ggACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATg                                                                                                                  >  1:190606/1‑140 (MQ=255)               cGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACata                                                                                                                              <  2:535014/122‑1 (MQ=255)               cGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACata                                                                                                                              >  1:535014/1‑122 (MQ=255)               cGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGaaa                                                                                                            >  1:299504/1‑140 (MQ=255)                       ttGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTg                                                                                                    <  2:339243/140‑1 (MQ=255)                                  aGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAagag                                                                                                       <  1:393940/126‑1 (MQ=255)                                  aGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAagag                                                                                                       >  2:393940/1‑126 (MQ=255)                                  aGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGc                                                                                         >  1:301553/1‑140 (MQ=255)                                         aaaTTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGgcg                                                                                  >  2:420589/1‑140 (MQ=255)                                         aaaTTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGgcg                                                                                  <  1:401015/140‑1 (MQ=255)                                         aaaTTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGgcg                                                                                  <  1:429027/140‑1 (MQ=255)                                         aaaTTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGgcg                                                                                  <  2:363456/140‑1 (MQ=255)                                         aaaTTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGgcg                                                                                  <  2:312782/140‑1 (MQ=255)                                              gAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACt                                                                             <  2:299828/140‑1 (MQ=255)                                                    gagaGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTc                                                                       <  1:230469/140‑1 (MQ=255)                                                                    agCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGt                                                       <  2:440227/140‑1 (MQ=255)                                                                      cgcgGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGGCt                                                     >  2:353215/1‑140 (MQ=255)                                                                      cgcgGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACAGTGGTCAGCCATTGCGGCGGGTCt                                                     >  2:245336/1‑140 (MQ=255)                                                                           cAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACAGTGGTCAGCCATTGCGGCGGGTCTGTCGa                                                <  1:245336/140‑1 (MQ=255)                                                                                 aatagaaatagaTGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGgcg                                          <  1:93488/140‑1 (MQ=255)                                                                                             tGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTACTgg                              <  2:179299/140‑1 (MQ=255)                                                                                                               ccGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAg            <  2:299504/140‑1 (MQ=255)                                                                                                                       tGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGGGCTTCGTTACTGGCAAAAAGGAGATTTTAAATCGAACTg    >  1:396039/1‑140 (MQ=255)                                                                                                                         gCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGa  <  1:36494/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                 GAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGA  >  NC_000913/2465295‑2465554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑