Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,477,075	T→C	R185R (AGA→AGG)	dsdC ←	DNA‑binding transcriptional dual regulator DsdC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,477,075	0	T	C	100.0%	122.3 / NA	34	R185R (AGA→AGG)	dsdC	DNA‑binding transcriptional dual regulator DsdC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/15);  total (19/15)

GAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCAT  >  NC_000913/2476938‑2477211                                                                                                                                          |                                                                                                                                         gaagaTGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCt                                                                                                                                        >  1:378466/1‑140 (MQ=255)  aagaTGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCtt                                                                                                                                       >  1:74200/1‑140 (MQ=255)  aagaTGTCGGCAAACTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCtt                                                                                                                                       <  2:505861/140‑1 (MQ=255)   agaTGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCttt                                                                                                                                      <  2:74200/140‑1 (MQ=255)       gTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGc                                                                                                                                  >  1:49337/1‑140 (MQ=255)         cGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGt                                                                                                                                >  1:294277/1‑140 (MQ=255)                 tAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCggg                                                                                                                        <  2:378466/140‑1 (MQ=255)                         tAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTcc                                                                                                                           >  2:406498/1‑129 (MQ=255)                         tAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTcc                                                                                                                           <  1:406498/129‑1 (MQ=255)                            tGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCggg                                                                                                                        >  1:284577/1‑129 (MQ=255)                            tGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCggg                                                                                                                        <  2:284577/129‑1 (MQ=255)                                   cccAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGAtt                                                                                                      >  1:403417/1‑140 (MQ=255)                                               aTTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTcatccatc                                                                                          <  2:330311/140‑1 (MQ=255)                                                 tCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAg                                                                                        <  2:49337/140‑1 (MQ=255)                                                           cGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGtgatga                                                                              <  2:175680/140‑1 (MQ=255)                                                             gAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGtga                                                                                 >  2:350223/1‑135 (MQ=255)                                                             gAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGtga                                                                                 <  1:350223/135‑1 (MQ=255)                                                                                 tctgtcATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGGATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAATGAGTTGCGCTGACGGCGcat                                                        >  2:491778/1‑140 (MQ=255)                                                                                    gtcATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGcatcat                                                     <  2:430875/140‑1 (MQ=255)                                                                                             cAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGa                                            >  1:126526/1‑140 (MQ=255)                                                                                               aCGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATc                                          >  2:21210/1‑140 (MQ=255)                                                                                               aCGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATc                                          >  1:470736/1‑140 (MQ=255)                                                                                                 gTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGc                                        <  2:126526/140‑1 (MQ=255)                                                                                                         acacAGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGa                                >  2:526630/1‑140 (MQ=255)                                                                                                          cacaGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGAt                               >  2:484277/1‑140 (MQ=255)                                                                                                          cacaGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGAt                               >  1:207449/1‑140 (MQ=255)                                                                                                          cacaGGTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGAt                               >  1:314324/1‑140 (MQ=255)                                                                                                              ggTTAATTACCGTGTTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGAttacg                           <  2:470736/140‑4 (MQ=255)                                                                                                                         gtgtTGGTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAg                >  2:42197/1‑140 (MQ=255)                                                                                                                               gTTAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGAc          >  1:211572/1‑140 (MQ=255)                                                                                                                                ttAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACg         <  2:12717/140‑1 (MQ=255)                                                                                                                                 tAAAGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGt        >  2:420092/1‑140 (MQ=255)                                                                                                                                  aaaGCATGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGtt       <  2:211572/140‑1 (MQ=255)                                                                                                                                       aTGCCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCAt  <  1:526630/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCAT  >  NC_000913/2476938‑2477211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑