Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,497,028	G→A	intergenic (+36/+29)	ypdK → / ← alaC	putative membrane protein YpdK/glutamate‑‑pyruvate aminotransferase AlaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,497,028	0	G	A	100.0%	124.7 / NA	37	intergenic (+36/+29)	ypdK/alaC	putative membrane protein YpdK/glutamate‑‑pyruvate aminotransferase AlaC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/14);  total (23/14)

CAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTT  >  NC_000913/2496896‑2497162                                                                                                                                     |                                                                                                                                       cAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGt                                                                                                                                 >  1:460236/1‑140 (MQ=255)                 agaTGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTgacgac                                                                                                                 <  1:243482/140‑1 (MQ=255)                 agaTGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTgacgac                                                                                                                 <  2:26753/140‑1 (MQ=255)                  gaTGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGacgacg                                                                                                                >  1:28143/1‑140 (MQ=255)                            gAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTc                                                                                                      >  2:524567/1‑140 (MQ=255)                             aaaTATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTgacgac                                                                                                                 <  2:462913/128‑1 (MQ=255)                             aaaTATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTgacgac                                                                                                                 >  1:462913/1‑128 (MQ=255)                             aaaTATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTcc                                                                                                     >  2:482801/1‑140 (MQ=255)                             aaaTATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTcc                                                                                                     >  2:42411/1‑140 (MQ=255)                              aaTATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTAtt                                                                                                       <  1:957/137‑1 (MQ=255)                              aaTATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTAtt                                                                                                       >  2:957/1‑137 (MQ=255)                                     cTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTc                                                                                             >  1:464711/1‑140 (MQ=255)                                                          ggTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGc                                                                        <  1:195654/140‑1 (MQ=255)                                                          ggTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGc                                                                        <  1:524567/140‑1 (MQ=255)                                                          ggTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGc                                                                        <  2:171628/140‑1 (MQ=255)                                                           gTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCg                                                                       >  1:232068/1‑140 (MQ=255)                                                              aTCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCgggg                                                                    >  2:430635/1‑139 (MQ=255)                                                                   ttGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTaa                                                               <  2:460236/140‑1 (MQ=255)                                                                           gTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATG‑AAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGc                                                      >  1:437856/1‑140 (MQ=255)                                                                             aCTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCa                                                     <  2:232068/140‑1 (MQ=255)                                                                                 ttGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGa                                                 >  1:68492/1‑140 (MQ=255)                                                                                     ccTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACAt                                             <  1:486682/140‑1 (MQ=255)                                                                                             tCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTaa                                     >  1:7429/1‑140 (MQ=255)                                                                                               tAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATc                                   <  2:157103/140‑1 (MQ=255)                                                                                                aaaaGCAAACTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATcc                                  <  1:369978/140‑1 (MQ=255)                                                                                                       aaCTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGAt                           >  1:22842/1‑140 (MQ=255)                                                                                                        aCTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTg                             <  1:323178/137‑1 (MQ=255)                                                                                                        aCTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTg                             >  2:323178/1‑137 (MQ=255)                                                                                                        aCTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATg                          >  1:449077/1‑140 (MQ=255)                                                                                                        aCTGTGCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATg                          >  1:409113/1‑140 (MQ=255)                                                                                                          tgtgCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGc                        <  2:68492/140‑1 (MQ=255)                                                                                                           gtgCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGGAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGcc                       >  1:109463/1‑140 (MQ=255)                                                                                                            tgCAATGAAAAACAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCt                      >  2:22042/1‑140 (MQ=255)                                                                                                                      aaCAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACg            >  2:295898/1‑140 (MQ=255)                                                                                                                      aaCAGGAGCCATCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACACCGCCTTAATCCCCCTGGTGGCCTGACGAATACg            >  2:471306/1‑140 (MQ=255)                                                                                                                                aTCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGttt  >  2:48650/1‑140 (MQ=255)                                                                                                                                aTCAGACTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGGAAACCGTCGGCACGGGACAACGCCTTTATCCCTCTGATGGCCTGGCGAATACGGTGGCGGgtt  >  2:418823/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                       CAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTT  >  NC_000913/2496896‑2497162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑