Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,636,979	A→T	D126E (GAT→GAA)	der ←	50S ribosomal subunit stability factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,636,979	0	A	T	100.0%	68.8 / NA	21	D126E (GAT→GAA)	der	50S ribosomal subunit stability factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (14/7);  total (14/7)

CGAGATCTTCCATCCACGGCAGCAGCACATGCTCCAGCAGACTTAATACGCCACGACCGTGAGACGCGGCGATCGGGTAGATTTCACCTAAACCAAGCGAGTAGAAATCAACCACTGCCTGATCGGGATCCAGACCGTCAGTTTTGTTTGCCACCAGGAAGGTCGGTTTTTCACGGGAGCGCAGATGTTTGGCAATCGCTTCATCTGCCGGCATCAGGCCCGCGCGCGCATCCACCATAAACAGTACGACGTCCG  >  NC_000913/2636858‑2637112                                                                                                                          |                                                                                                                                      cGAGATCTTCCATCCACGGCAGCAGCACATGCTCCAGCAGACTTAATACGCCACGACCGTGAGACGCGGCGATCGGGTAGATTTCACCTAAACCAAGCGAGTAGAAATCAACCACTGCCTGTTCGGGATCCAGACCGTCa                                                                                                                     >  1:243635/1‑140 (MQ=255)              ccaCGGCAGCAGCACATGCTCCAGCAGACTTAATACGCCACGACCGTGAGACGCGGCGATCGGGTAGATTTCACCTAAACCAAGCGAGTAGAAATCAACCACTGCCTGTTCGGGATCCAGACCGTCAGTTTTGTTTGcca                                                                                                        >  1:23359/1‑140 (MQ=255)                            caTGCTCCAGCAGACTTAATACGCCACGACCGTGAGACGCGGCGATCGGGTAGATTTCACCTAAACCAAGCGAGTAGAAATCAACCACTGCCTGTTCGGGATCCAGACCGTCAGTTTTGTTTGCCACCAGGAAGGTCGGt                                                                                          >  1:249387/1‑140 (MQ=255)                                                   ccACGACCGTGAGACGCGGCGATCGGGTAGATTTCACCTAAACCAAGCAAGTAGAAATCAACCACTGCCTGTTCGGGATCCAGACCGTCAGTTTTGTTTGCCACCAGGAAGGTCGGTTTTTCACGGGAGCGCAGATGttt                                                                   >  1:491138/1‑140 (MQ=255)                                                    taCGACCGTGAGACGCGGCGATCGGGTAGATTTCACCTAAACCAAGCGAGTAGAAATCAACCACTGCCTGTTCGGGATCCAGACCGTCAGTTTTGTTTGCCACCAGGAAGGTCGGTTTTTCACGGGAGCGCAGATGTTTg                                                                  >  1:133436/2‑140 (MQ=255)                                                     aCGACCGTGAGACGCGGCGATCGGGTAGATTTCACCTAAACCAAGCGAGTAGAAATCAACCACTGCCTGTTCGGGATCCAGACCGTCAGTTTTGTTTGCCACCAGGAAGGTCGGTTTTTCACGGGAGCGCAGATGTTTgg                                                                 >  1:13596/1‑140 (MQ=255)                                                     aCGACCGTGAGACGCGGCGATCGGGTAGATTTCACCTAAACCAAGCGAGTAGAAATCAACCACTGCCTGTTCGGGATCCAGACCGTCAGTTTTGTTTGCCACCAGGAAGGTCGGTTTTTCACGGGAGCGCAGATGTTTgg                                                                 >  2:495367/1‑140 (MQ=255)                                                       gACCGTGAGACGCGGCGATCGGGTAGATTTCACCTAAACCAAGCGAGTAGAAATCAACCACTGCCTGTTCGGGATCCAGACCGTCAGTTTTGTTTGCCACCAGGAAGGTCGGTTTTTCACGGGAGCGCAGATGTTTGGCa                                                               >  2:414724/1‑140 (MQ=255)                                                       gACCGTGAGACGCGGCGATCGGGTAGATTTCACCTAAACCAAGCGAGTAGAAATCAACCACTGCCTGTTCGGGATCCAGACCGTCAGTTTTGTTTGCCACCAGGAAGGTCGGTTTTTCACGGGAGCGCAGATGTTTGGCa                                                               <  2:243635/140‑1 (MQ=255)                                                       gACCGTGAGACGCGGCGATCGGGTAGATTTCACCTAAACCAAGCGAGTAGAAATCAACCACTGCCTGTTCGGGATCCAGACCGTCAGTTTTGTTTGCCACCAGGAAGGTCGGTTTTTCACGGGAGCGCAGATGTTTGGCa                                                               <  2:133436/140‑1 (MQ=255)                                                                 cgcgGCGATCGGGTAGATTTCACCTAAACCAAGCGAGTAGAAATCAACCACTGCCTGTTCGGGATCCAGACCGTCAGTTTTGTTTGCCACCAGGAAGGTCGGTTTTTCACGGGAGCGCAGATGTTTGGCAATCGCTTCAt                                                     >  1:449135/1‑140 (MQ=255)                                                                      cgATCGGGTAGATTTCACCTAAACCAAGCGAGTAGAAATCAACCACTGCCTGTTCGGGATCCAGACCGTCAGTTTTGTTTGCCACCAGGAAGGTCGGTTTTTCACGGGAGCGCAGATGTTTGGCAATCGCTTCATCTGcc                                                <  2:64306/140‑1 (MQ=255)                                                                      cgATCGGGTAGATTTCACCTAAACCAAGCGAGTAGAAATCAACCACTGCCTGTTCGGGATCCAGACCGTCAGTTTTGTTTGCCACCAGGAAGGTCGGTTTTTCACGGGAGCGCAGATGTTTGGCAATCGCTTCATCTGcc                                                <  1:79717/140‑1 (MQ=255)                                                                      cgATCGGGTAGATTTCACCTAAACCAAGCGAGTAGAAATCAACCACTGCCTGTTCGGGATCCAGACCGTCAGTTTTGTTTGCCACCAGGAAGGTCGGTTTTTCACGGGAGCGCAGATGTTTGGCAATCGCTTCATCTGcc                                                <  1:495367/140‑1 (MQ=255)                                                                           gggTAGATTTCACCTAAACCAAGCGAGTAGAAATCAACCACTGCCTGTTCGGGATCCAGACCGTCAGTTTTGTTTGCCACCAGGAAGGTCGGTTTTTCACGGGAGCGCAGATGTTTGGCAATCGCTTCATCTGCCGGCAt                                           <  2:410470/140‑1 (MQ=255)                                                                                           aaCCAAGCGAGTAGAAATCAACCACTGCCTGTTCGGGATCCAGACCGTCAGTTTTGTTTGCCACCAGGAAGGTCGGTTTTTCACGGGAGCGCAGATGTTTGGCAATCGCTTCATCTGCCGGCATCAGGCCCGCGCGCGCa                           >  1:220473/1‑140 (MQ=255)                                                                                                aGCGAGTAGAAATCAACCACTGCCTGTTCGGGATCCAGACCGTCAGTTTTGTTTGCCACCAGGAAGGTCGGTTTTTCACGGGAGCGCAGATGTTTGGCAATCGCTTCATCTGCCGGCATCAGGCCCGCGCGCGCATccac                      >  1:488080/1‑140 (MQ=255)                                                                                                aGCGAGTAGAAATCAACCACTGCCTGTTCGGGATCCAGACCGTCAGTTTTGTTTGCCACCAGGAAGGTCGGTTTTTCACGGGAGCGCAGATGTTTGGCAATCGCTTCATCTGCCGGCATCAGGCCCGCGCGCGCATccac                      >  1:246861/1‑140 (MQ=255)                                                                                                    aGTAGAAATCAGCCACTGCCTGTTCGGGATCCAGACCGTCAGTTTTGTTTGCCACCAGGAAGGTCGGTTTTTCACGGGAGCGCAGATGTTTGGCAATCGCTTCATCTGCCGGCATCAGGCCCGCGTGCGCATCCGCCATa                  <  2:297265/140‑1 (MQ=255)                                                                                                    aGTAGAAATCAACCACTGCCTGTTCGGGATCCAGACCGTCAGTTTTGTTTGCCACCAGGAAGGTCGGTTTTTCACGGGAGCGCAGATGTTTGGCAATCGCTTCATCTGACGGCATCAGGCCCGCGCGCGCATCCACCATa                  <  2:23359/140‑1 (MQ=255)                                                                                                       aGAAATCAACCCCTGCCTGTTCGGGATCCAGACCGTCAGTTTTGTTTGCCACCAGGAAGGTCGGTTTTTCACGGGAGCGCAGATGTTTGGCAATCGCTTCATCTGCCGGCATCAGGCCCGCGCGCGCATCCACCATAAAc               <  2:390794/140‑1 (MQ=255)                                                                                                                ccACTGCCTGTTCGGGATCCAGACCGTCAGTTTTGTTTGCCACCAGGAAGGTCGGTTTTTCACGGGAGCGCAGATGTTTGGCAATCGCTTCATCTGCCGGCATCAGGCCCGCGCGCGCATCCACCATAAACAGTacgacg      >  1:493446/1‑140 (MQ=255)                                                                                                                    tGCCTGTTCGGGATCCAGACCGTCAGTTTTGTTTGCCACCAGGAAGGTCGGTTTTTCACGGGAGCGCAGATGTTTGGCAATCGCTTCATCTGCCGGCATCAGGCCCGCGCGCGCATCCACCATAAACAGTACGACGTCCg  >  1:405529/1‑140 (MQ=255)                                                                                                                          |                                                                                                                                      CGAGATCTTCCATCCACGGCAGCAGCACATGCTCCAGCAGACTTAATACGCCACGACCGTGAGACGCGGCGATCGGGTAGATTTCACCTAAACCAAGCGAGTAGAAATCAACCACTGCCTGATCGGGATCCAGACCGTCAGTTTTGTTTGCCACCAGGAAGGTCGGTTTTTCACGGGAGCGCAGATGTTTGGCAATCGCTTCATCTGCCGGCATCAGGCCCGCGCGCGCATCCACCATAAACAGTACGACGTCCG  >  NC_000913/2636858‑2637112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑