Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,694,519	A→G	I342T (ATT→ACT)	purL ←	phosphoribosylformylglycinamide synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,694,519	0	A	G	100.0%	58.6 / NA	18	I342T (ATT→ACT)	purL	phosphoribosylformylglycinamide synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/6);  total (12/6)

CCGGACGACCAAATTCGTTGTTAAACGCCGCGCCGCCCAGCGGGCCTTCGGTCATGATGTCCAGCGCGGTGACAATGCGCTCAGGCTTACCGAAATCTTCTTCCCACGGCTGTTCGAAGCCAGGAATTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCG  >  NC_000913/2694394‑2694645                                                                                                                              |                                                                                                                               ccGGACGACCAAATTCGTTGTTAAACGCCGCGCCGCCCAGCGGGCCTTCGGTCATGATGTCCAGCGCGGTGACAATGCGCTCAGGCTTACCGAAATCTTCTTCCCACGGCTGTTCGAAGCCAGGAGTTCGCAGGTTGGaa                                                                                                                  >  2:338179/1‑140 (MQ=255)         ccAAATTCGTTGTTAAACGCCGCGCCGCCCAGCGGGCCTTCGGTCATGATGTCCAGCGCGGTGACAATGCGCTCAGGCTTACCGAAATCTTCTTCCCACGGCTGTTCGAAGCCAGGAGTTCGCAGGTTGGAAACGGAGaa                                                                                                          <  1:359603/140‑1 (MQ=255)                                                                gcgcGGTGACAATGCGCTCAGGCTTACCGAAATCTTCTTCCCACGGCTGTTCGAAGCCAGGAGTTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCgg                                                   >  2:236855/1‑140 (MQ=255)                                                                 cgcgGCGACAATGCGCTCAGGCTTACCGAAATCTTCTTCCCACGGCTGTTCGAAGCCAGGAGTTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGa                                                  >  2:5759/1‑140 (MQ=255)                                                                      tGACAATGCGCTCAGGCTTACCGAAATCTTCTTCCCACGGCTGTTCGAAGCCAGGAGTTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCa                                             >  2:318928/1‑140 (MQ=255)                                                                      ggACAATGCGCTCAGGCTTACCGAAATCTTCTTCCCACGGCTGTTCGAAGCCAGGAGTTCGCAGGTTGGAAACGGAGAAACCACCCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCa                                             <  2:308284/139‑1 (MQ=255)                                                                                cTCAGGCTTACCGAAATCTTCTTCCCACGGCTGTTCGAAGCCAGGAGTTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAAc                                   >  1:221541/1‑140 (MQ=255)                                                                                cTCAGGCTTACCGAAATCTTCTTCCCACGGCTGTTCGAAGCCAGGAGTTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAAc                                   <  1:55887/140‑1 (MQ=255)                                                                                    ggCTTACCGAAATCTTCTTCCCACGGCTGTTCGAAGCCAGGAGTTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGt                               >  1:326467/1‑140 (MQ=255)                                                                                             aaaTCTTCTTCCCACGGCTGTTCGAAGCCAGGAGTTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGcc                      <  2:221541/140‑1 (MQ=255)                                                                                               aTCTTCTTCCCACGGCTGTTCGAAGCCAGGAGTTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCcgcg                        <  2:59741/136‑1 (MQ=255)                                                                                               aTCTTCTTCCCACGGCTGTTCGAAGCCAGGAGTTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCcgcg                        >  1:59741/1‑136 (MQ=255)                                                                                                       cccACGGCTGTTCGAAGCCAGGAGTTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGga            >  2:113852/1‑140 (MQ=255)                                                                                                            ggCTGTTCGAAGCCAGGAGTTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAAt       >  2:66226/1‑140 (MQ=255)                                                                                                               tGTTCGAAGCCAGGAGTTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGccc                     >  2:251774/1‑123 (MQ=255)                                                                                                               tGTTCGAAGCCAGGAGTTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGccc                     <  1:251774/123‑1 (MQ=255)                                                                                                                 ttCGAAGCCAGGAGTTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCg  >  2:411260/1‑140 (MQ=255)                                                                                                                 ttCGAAGCCAGGAGTTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCg  >  1:486569/1‑140 (MQ=255)                                                                                                                              |                                                                                                                               CCGGACGACCAAATTCGTTGTTAAACGCCGCGCCGCCCAGCGGGCCTTCGGTCATGATGTCCAGCGCGGTGACAATGCGCTCAGGCTTACCGAAATCTTCTTCCCACGGCTGTTCGAAGCCAGGAATTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCG  >  NC_000913/2694394‑2694645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑