Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,779,579	T→C	T1050A (ACC→GCC)	ypjA ←	adhesin‑like autotransporter YpjA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,779,579	0	T	C	100.0%	93.8 / NA	28	T1050A (ACC→GCC)	ypjA	adhesin‑like autotransporter YpjA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/15);  total (13/15)

CCGCTACCGGAAAGTTCATTGGTTGTCAGCGTATTGTAGATACCGTTAGTTGTGGCAGGCGCTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACTGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGT  >  NC_000913/2779443‑2779712                                                                                                                                         |                                                                                                                                      ccGCTACCGGAAAGTTCATTGGTTGTCAGCGTATTGTAGATGCCGTTAGTTGAGGCAGCCGCTGCTGGTTGCTGTGGCAAATAAACATCAACGTAGTGGAGTAGCAAAATTTTTACTCTATATTCGGAGGTGCCGGCAcc                                                                                                                                    >  2:227629/1‑140 (MQ=255) ccGCTACCGGAAAGTTCATTGGTTGTCAGCGTATTGTAGATACCGTTAGTTGTGGCAGGCGCTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGCAcc                                                                                                                                    >  1:418543/1‑140 (MQ=255)  cGCTACCGGAAAGTTCATTGGTTGTCAGCGTATTGTAGATACCGTTAGTTGTGGCAGGCGCTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGCACCt                                                                                                                                   >  1:537655/1‑140 (MQ=255)      aCCGGAAAGTTCATTGGTTGTCAGCGTATTGTAGATACCGTTAGTTGTGGCAGGCGCTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGCACCTGcac                                                                                                                               <  1:356414/140‑1 (MQ=255)       ccGGAAAGTTCATTGGTTGTCAGCGTATTGTAGATACCGTTAGTTGTGGCAGGCGCTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGCACCTGcaca                                                                                                                              <  2:536002/140‑1 (MQ=255)       ccGGAAAGTTCATTGGTTGTCAGCGTATTGTAGATACCGTTAGTTGTGGCAGGCGCTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGCACCTGcaca                                                                                                                              <  1:427157/140‑1 (MQ=255)               ttCATTGGTTGTCAGCGTATTGTAGATACCGTTAGTTGTGGCAGGCGCTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGCACCTGCACAGGAattat                                                                                                                      >  2:206963/1‑140 (MQ=255)                tCATTGGTTGTCAGCGTATTGTAGATACCGTTAGTTGTGGCAGGCGCTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGCACCTGCACAGGAattatt                                                                                                                     <  1:479974/140‑1 (MQ=255)                         gTCAGCGTATTGTAGATACCGTTAGTTGTGGCAGGCGCTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGCACCTGCACAGGAATTATTGCTGTTAAg                                                                                                            >  1:56002/1‑140 (MQ=255)                           cAGCGTATTGTAGATACCGTTAGTTGTGGCAGGCGCTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGCACCTGCACAGGAATTATTGCTGTTAAGcc                                                                                                          <  2:55342/140‑1 (MQ=255)                              cGTATTGTAGATACCGTTAGTTGTGGCAGGCGCTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGCACCTGCACAGGAATTATTGCTGTTAAGCCAGa                                                                                                       <  1:450860/140‑1 (MQ=255)                                      aGATACCGTTAGTTGTGGCAGGCGCTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGCACCTGCACAGGAATTATTGCTGTTAAGCCAGACACTGccc                                                                                               <  1:206963/140‑1 (MQ=255)                                           ccGTTAGTTGTGGCAGGCGCTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGCACCTGCACAGGAATTATTGCTGTTAAGCCAGACACTGCCCCGGCt                                                                                          >  2:217063/1‑140 (MQ=255)                                            cGTTAGTTGTGGCAGGCGCTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGCACCTGCACAGGAATTATTGCTGTTAAGCCAGACACTGCCCCGGCTg                                                                                         >  1:344731/1‑140 (MQ=255)                                                  ttGTGGCAGGCGCTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGCACCTGCACAGGAATTATTGCTGTTAAGCCAGACACTGCCCCGGCTgg                                                                                        >  1:369736/1‑135 (MQ=255)                                                  ttGTGGCAGGCGCTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGCACCTGCACAGGAATTATTGCTGTTAAGCCAGACACTGCCCCGGCTgg                                                                                        <  2:369736/135‑1 (MQ=255)                                                            cgcTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGCACCTGCACAGGAATTATTGCTGTTAAGCCAGACACTGCCCCGGCTGGCAGCCGTCAGGTCgg                                                                         >  1:103369/1‑140 (MQ=255)                                                             gctgctGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGCACCTGCACAGGAATTATTGCTGTTAAGCCAGACACTGCCCCGGCTGGCAGACGTCAGGTCGGc                                                                        <  2:367075/140‑1 (MQ=255)                                                                         gCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGCACCTGCACAGGAATTATTGCTGTTAAGCCAGACACTGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATc                                                            >  1:41477/1‑140 (MQ=255)                                                                                    aaCATCACCGTCGTTGAGTAGCAAACTGTTTGCTCTATATTCGCAGTTGCTGGCACCTGCACAGGAATTATTGCTGTTAAGCCAGACACTGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTc                                                 >  1:119011/1‑140 (MQ=255)                                                                                      cATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGCACCTGCACAGGAATTATTGCTGTTAAGCCAGACACTGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTccc                                               <  2:103369/140‑1 (MQ=255)                                                                                      cATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGCACCTGCACAGGAATTATTGCTGTTAAGCCAGACACTGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTccc                                               <  1:254734/140‑1 (MQ=255)                                                                                      cATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGCACCTGCACAGGAATTATTGCTGTTAAGCCAGACACTGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTccc                                               <  2:41477/140‑1 (MQ=255)                                                                                                       aGCAAACTGTTTACTCTATATTCGCAGTTGCTGGCACCTGCACAGGAATTATTGCTGTTAAGCCAGACACTGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCt                              <  2:418543/140‑1 (MQ=255)                                                                                                       aGCAAACTGTTTACTCTATATTCGCAGTTGCTGGCACCTGCACAGGAATTATTGCTGTTAAGCCAGACACTGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCt                              <  2:56002/140‑1 (MQ=255)                                                                                                             cTGTTTACTCTATATTCGCAGTTGCTGGCACCTGCACAGGAATTATTGCTGTTAAGCCAGACACTGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGt                        >  1:76/1‑140 (MQ=255)                                                                                                                     tctATATTCGCAGTTGCTGGCACCTGCACAGGAATTATTGCTGTTAAGCCAGACACTGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGa                <  2:537655/140‑1 (MQ=255)                                                                                                                          atTCGCAGTTGCTGGCACCTGCACAGGAATTATTGCTGTTAAGCCAGACACTGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGcc           <  1:486355/140‑1 (MQ=255)                                                                                                                                   tGCTGGCACCTGCACAGGAATTATTGCTGTTAAGCCAGACACTGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGt  >  2:340349/1‑140 (MQ=255)                                                                                                                                   tGCTGGCACCTGCACAGGAATTATTGCTGTTAAGCCAGACACTGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGt  >  1:73828/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      CCGCTACCGGAAAGTTCATTGGTTGTCAGCGTATTGTAGATACCGTTAGTTGTGGCAGGCGCTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACTGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGT  >  NC_000913/2779443‑2779712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑