Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,795,349	G→A	S366N (AGC→AAC)	gabP →	4‑aminobutyrate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,795,349	0	G	A	100.0%	88.8 / NA	27	S366N (AGC→AAC)	gabP	4‑aminobutyrate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/9);  total (18/9)

AATCAACCGCAGTAAAACCCCGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATC  >  NC_000913/2795233‑2795485                                                                                                                     |                                                                                                                                         aaTCAACCGCAGTAAAACCCCGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCctgct                                                                                                                     <  2:421555/138‑1 (MQ=255) aaTCAACCGCAGTAAAACCCCGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCctgct                                                                                                                     >  1:421555/1‑138 (MQ=255) aaTCAACCGCAGTAAAACCCCGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCctg                                                                                                                       <  1:17551/136‑1 (MQ=255) aaTCAACCGCAGTAAAACCCCGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCctg                                                                                                                       >  2:17551/1‑136 (MQ=255) aaTCAACCGCAGTAAAACCCCGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTgg                                                                                                                   >  1:317963/1‑140 (MQ=255)                                 tgtTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCgg                                                                                   >  2:416088/1‑140 (MQ=255)                                        ctcCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCa                                                                            >  1:149712/1‑140 (MQ=255)                                             ccGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAAtt                                                                       >  2:415353/1‑140 (MQ=255)                                                gcgcGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTg                                                                    >  1:476901/1‑140 (MQ=255)                                                   cgGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGa                                                                 >  1:46360/1‑140 (MQ=255)                                                                     tggtgAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAAtt                                               >  1:80348/1‑140 (MQ=255)                                                                     tggtgAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAAtt                                               >  2:73761/1‑140 (MQ=255)                                                                     tggtgAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAAtt                                               >  2:427012/1‑140 (MQ=255)                                                                      ggtgAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTc                                              <  2:80348/140‑1 (MQ=255)                                                                         gAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCt                                           <  1:13878/140‑1 (MQ=255)                                                                                tACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAAt                                    <  1:427012/140‑1 (MQ=255)                                                                                    cgcCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTgg                                >  1:255676/1‑140 (MQ=255)                                                                                     gcCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGc                               <  2:220277/140‑1 (MQ=255)                                                                                       cTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCtt                             >  2:157398/1‑140 (MQ=255)                                                                                         gCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTa                           >  2:213558/1‑140 (MQ=255)                                                                                         gCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTa                           >  2:399350/1‑140 (MQ=255)                                                                                                       aTTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCt             >  1:354645/1‑140 (MQ=255)                                                                                                          tCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGc          >  1:29633/1‑140 (MQ=255)                                                                                                                cGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCa    >  1:175126/1‑140 (MQ=255)                                                                                                                  aCAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATc  <  2:21729/140‑1 (MQ=255)                                                                                                                  aCAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATc  <  2:476901/140‑1 (MQ=255)                                                                                                                  aCAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATc  <  1:157398/140‑1 (MQ=255)                                                                                                                     |                                                                                                                                         AATCAACCGCAGTAAAACCCCGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATC  >  NC_000913/2795233‑2795485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑