Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,803,182	G→A	R612H (CGC→CAC)	nrdE →	ribonucleoside‑diphosphate reductase 2 subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,803,182	0	G	A	100.0%	121.6 / NA	36	R612H (CGC→CAC)	nrdE	ribonucleoside‑diphosphate reductase 2 subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/17);  total (19/17)

CGCAGCTGCGCGACGACGTGATGCGCTACGGCATATACAACCAGAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACGCAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATG  >  NC_000913/2803047‑2803316                                                                                                                                        |                                                                                                                                       cgcAGCTGCGCGACGACGTGATGCGCTACGGCATATACAACCAGAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACaaa                                                                                                                                    <  2:366828/140‑1 (MQ=255)    aGCTGCGCGACGACGTGATGCGCTACGGCATATACAACCAGAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAaga                                                                                                                                  >  1:223217/1‑139 (MQ=255)    aGCTGCGCGACGACGTGATGCGCTACGGCATATACAACCAGAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAaga                                                                                                                                  <  2:223217/139‑1 (MQ=255)           cgacgaCGTGATGCGCTACGGCATATACAACCAGAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCaaaa                                                                                                                          >  1:492496/1‑140 (MQ=255)                   tGATGCGCTACGGCATATACAACCAGAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGAcgc                                                                                                                  <  2:427737/140‑1 (MQ=255)                                  tataCAACCAGAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGcc                                                                                                   >  2:347723/1‑140 (MQ=255)                                  tataCAACCAGAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGcc                                                                                                   >  1:507185/1‑140 (MQ=255)                                       aaCCAGAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGtt                                                                                              >  1:137508/1‑140 (MQ=255)                                        aCCAGAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGttt                                                                                             >  1:423463/1‑140 (MQ=255)                                            gAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGa                                                                                         >  2:153981/1‑140 (MQ=255)                                             aaTCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGAc                                                                                        >  1:402594/1‑140 (MQ=255)                                             aaTCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGAc                                                                                        >  1:493983/1‑140 (MQ=255)                                                         gTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCt                                                                            >  1:392691/1‑140 (MQ=255)                                                           gccgccAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTgg                                                                          <  2:392691/140‑1 (MQ=255)                                                             cgccAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGgcg                                                                        <  1:153981/140‑1 (MQ=255)                                                             cgccAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGgcg                                                                        <  2:423463/140‑1 (MQ=255)                                                             cgccAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGgcg                                                                        <  1:237257/140‑1 (MQ=255)                                                                                 tataTCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGa                                                    >  1:25421/1‑140 (MQ=255)                                                                                      cAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTg                                               >  1:22/1‑140 (MQ=255)                                                                                      cAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTg                                               <  2:385940/140‑1 (MQ=255)                                                                                        aCCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGgc                                             >  2:488746/1‑140 (MQ=255)                                                                                        aCCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGgc                                             >  2:416457/1‑140 (MQ=255)                                                                                          cATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGgcgc                                           >  1:22176/1‑140 (MQ=255)                                                                                           aTGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCa                                          <  1:416457/140‑1 (MQ=255)                                                                                           aTGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCa                                          <  2:402594/140‑1 (MQ=255)                                                                                                  gTCGAGTATTCATCCGATTGTCGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGa                                   <  2:492496/140‑1 (MQ=255)                                                                                                   tCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGat                                  >  1:345346/1‑140 (MQ=255)                                                                                                       gTATTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGatcatc                              >  1:27320/1‑140 (MQ=255)                                                                                                         aTTCATCCGATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGa                            <  2:345346/140‑1 (MQ=255)                                                                                                                 gATTGTGGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTAcg                    <  1:68421/140‑1 (MQ=255)                                                                                                                    tgtgGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAACTGGCGCAGAAAAGATCATCGACACCTACGCgg                 >  2:398826/1‑140 (MQ=255)                                                                                                                      tgGCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGaa               <  2:22/140‑1 (MQ=255)                                                                                                                        gCGAAAGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGc             >  1:16339/1‑140 (MQ=255)                                                                                                                             aGTAGAGATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTc        <  1:488746/140‑1 (MQ=255)                                                                                                                                agagATACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGcc     <  1:300159/140‑1 (MQ=255)                                                                                                                                   gaTACACAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATg  <  2:16339/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       CGCAGCTGCGCGACGACGTGATGCGCTACGGCATATACAACCAGAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACGCAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATG  >  NC_000913/2803047‑2803316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑