Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	226,952	A→G	noncoding (1194/2904 nt)	rrlH →	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	226,952	0	A	G	100.0%	46.3 / NA	16	noncoding (1194/2904 nt)	rrlH	23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/9);  total (7/9)

AGCCATCATTTAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATC  >  NC_000913/226831‑227088                                                                                                                          |                                                                                                                                         agcCATCATTTAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGGGCGTTCTGTAAGCCtgtg                                                                                                                        >  2:51793/1‑140 (MQ=14)   ccATCATTTAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGGGCGTTCTGTAAGCCTGTGaa                                                                                                                      <  1:421627/140‑1 (MQ=18)      tcatTTAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGGGCGTTCTGTAAGCCTGTGAAGgt                                                                                                                   <  2:462210/140‑1 (MQ=18)         tttAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGGGCGTTCTGTAAGCCTGTGAAGGTGTa                                                                                                                <  1:201781/140‑1 (MQ=18)                  agCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGGGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGt                                                                                                       >  2:316592/1‑140 (MQ=25)                        aTAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGGGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTg                                                                                                 <  1:51793/140‑1 (MQ=255)                        aTAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGGGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTg                                                                                                 <  2:264165/140‑1 (MQ=255)                               aCTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGGGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTAt                                                                                          >  1:98758/1‑140 (MQ=37)                                   gTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGGGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGa                                                                                      <  2:98758/140‑1 (MQ=255)                                             ccTGCGCGGAAGATGTAACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGGGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATg                                                                            >  2:22955/1‑140 (MQ=37)                                                  gcgGAAGATGTAACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGGGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGAc                                                                       <  2:294880/140‑1 (MQ=255)                                                   cgGAAGATGTAACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGGGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACa                                                                      <  2:479118/140‑1 (MQ=37)                                                                            tGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGGGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGaaaa                                             >  1:298947/1‑140 (MQ=37)                                                                                             aGCGACGCTTATGCGTTGTTGGGTAGGGGGGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGa                            <  2:298947/140‑1 (MQ=37)                                                                                             aGCGACGCTTATGCGTTGTTGGGTAGGGGGGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGATGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCGCGCCGGAAGa                            <  2:149613/140‑1 (MQ=255)                                                                                             aGCGACGCTTATGCGTTGTTGGGTAGGGGGGCGTTCTGTAAGCCTGTGAAGGTGGACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGa                            >  2:163708/1‑140 (MQ=255)                                                                                                                 gggTAGGGGGGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACg        >  1:76645/1‑140 (MQ=21)                                                                                                                      ggggggCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAAt   >  2:229902/1‑140 (MQ=21)                                                                                                                       ggggGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATc  <  1:163708/140‑1 (MQ=21)                                                                                                                          |                                                                                                                                         AGCCATCATTTAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATC  >  NC_000913/226831‑227088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑