Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,973,440	T→C	E141E (GAA→GAG)	lplT ←	lysophospholipid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,973,440	0	T	C	100.0%	41.3 / NA	13	E141E (GAA→GAG)	lplT	lysophospholipid transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/4);  total (9/4)

TTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTCCATTAAACCGTTAGCTTTCACTAACTTACTACCCGTGGTTAATTCGCCGAGAATACCGTATTTCGCCGGTGAATAGGCTGCAGCACCAACACCCACCAGCGTATAGCCGAGAAACGGATTGATACCAAAG  >  NC_000913/2973321‑2973572                                                                                                                        |                                                                                                                                     ttGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACTCCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCCTCCATTAAACCGTTAGCttt                                                                                                                  <  1:472331/140‑1 (MQ=255)                              tGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCCTCCATTAAACCGTTAGCTTTCACTAACTTACTACCCGTGGTTAATTCGc                                                                                     >  1:55059/1‑140 (MQ=255)                              tGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCCTCCATTAAACCGTTAGCTTTCACTAACTTACTACCCGTGGTTAATTCGc                                                                                     >  2:30753/1‑140 (MQ=255)                              tGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCCTCCATTAAACCGTTAGCTTTCACTAACTTACTACCCGTGGTTAATTCGc                                                                                     >  2:536579/1‑140 (MQ=255)                                    tGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCCTCCATTAAACCGTTAGCTTTCACTAACTTACTACCCGTGGTTAATTCGCCGAGaa                                                                               <  1:30753/140‑1 (MQ=255)                                                  cgaGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCCTCCATTAAACCGTTAGCTTTCACTAACTTACTACCCGTGGTTAATTCGCCGAGAATACCGTATTTCGcc                                                                 >  2:228545/1‑140 (MQ=255)                                                                tCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCCTCCATTAAACCGTTAGCTTTCACTAACTTACTACCCGTGGTTAATTCGCCGAGAATACCGTATTTCGCCGGTGAATAGGCTgc                                                   >  1:7642/1‑140 (MQ=255)                                                                tCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCCTCCATTAAACCGTTAGCTTTCACTAACTTACTACCCGTGGTTAATTCGCCGAGAATACCGTATTTCGCCGGTGAATAGGCTgc                                                   >  2:66919/1‑140 (MQ=255)                                                                             caccGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCCTCCATTAAACCGTTAGCTTTCACTAACTTACTACCCGTGGTTAATTCGCCGAGAATACCGTATTTCGCCGGTGAATAGGCTGCAGCACCAAcaccc                                      >  2:221989/1‑140 (MQ=255)                                                                                  gCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCCTCCATTAAACCGTTAGCTTTCACTAACTTACTACCCGTGGTTAATTCGCCGAGAATACCGTATTTCGCCGGTGAATAGGCTGCAGCACCAACACCCACCAg                                 >  2:466234/1‑140 (MQ=255)                                                                                              aGCAAAATCGCCGCTATGGTAGAAGCCTCCATTAAACCGTTAGCTTTCACTAACTTACTACCCGTGGTTAATTCGCCGAGAATACCGTATTTCGCCGGTGAATAGGCTGCAGCACCAACACCCACCAGCGTATAGCCgag                     >  2:342543/1‑140 (MQ=255)                                                                                                cAAAATCGCCGCTATGGTAGAAGCCTCCATTAAACCGTTAGCTTTCACTAACTTACTACCCGTGGTTAATTCGCCGAGAATACCGTATTTCGCCGGTGAATAGGCTGCAGCACCAACACCCACCAGCGTATAGCCGAGaa                   >  1:342710/1‑140 (MQ=255)                                                                                                      cgccgcTATGGTAGAAGCCTCCATTAAACCGTTAGCTTTCACTAACTTACTACCCGTGGTTAATTCGCCGAGAATACCGTATTTCGCCGGTGAATAGGCTGCAGCACCAACACCCACCAGCGTATAGCCGAGAAACGGAt             <  1:228545/140‑1 (MQ=255)                                                                                                           cTATGGTAGAAGCCTCCATTAAACCGTTAGCTTTCACTAACTTACTACCCGTGGTTAATTCGCCGAGAATACCGTATTTCGCCGGTGAATAGGCTGCAGCACCAACACCCACCAGCGTATAGCCGAGAAACGGATTGATa        <  2:342710/140‑1 (MQ=255)                                                                                                                 tAGAAGCCTCCATTAAACCGTTAGCTTTCACTAACTTACTAGCCGTGGTTAATTCGCCGAGAATACCGTATTTCGCCGGTGAATAGGCTGCAGCACCAACACCCACCAGCGTATAGCCGAGAAACGGATTGATACCAAAg  <  1:536579/140‑1 (MQ=255)                                                                                                                        |                                                                                                                                     TTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTCCATTAAACCGTTAGCTTTCACTAACTTACTACCCGTGGTTAATTCGCCGAGAATACCGTATTTCGCCGGTGAATAGGCTGCAGCACCAACACCCACCAGCGTATAGCCGAGAAACGGATTGATACCAAAG  >  NC_000913/2973321‑2973572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑