Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,992,984	T→C	C297C (TGT→TGC)	ygeH →	putative transcriptional regulator YgeH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,992,984	0	T	C	100.0%	177.5 / NA	48	C297C (TGT→TGC)	ygeH	putative transcriptional regulator YgeH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/20);  total (28/20)

AATCATTATTTATCAGATGAAATGTTAGCCGGAAAGAAAGAACTTTACGACTTCACCCCTGAAAGCATTTACAGGGCCATGACTATATTTGATAGATTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGTTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCTTTTAGATTATGTATCAGACATAACCACTGTCGATGGAAAAATTTTAGCT  >  NC_000913/2992847‑2993119                                                                                                                                          |                                                                                                                                        aaTCATTATTTATCAGATGAAATGTTAGCCGGAAAGAAAGAACTTTACGACTTCACCCCTGAAAGCATTTACAGGGCCATGACTATATTTGATAGATTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGCTa                                                                                                                                       >  2:98081/1‑140 (MQ=255)         tttatCAGATGAAATGTTAGCCGGAAAGAAAGAACTTTACGACTTCACCCCTGAAAGCATTTACAGGGCCATGACTATATTTGATAGATTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTc                                                                                                                               >  1:135576/1‑140 (MQ=255)                 aTGAAATGTTAGCCGGAAAGAAAGAACTTTACGACTTCACCCCTGAAAGCATTTACAGGGCCATGACTATATTTGATAGATTACAAAATAAAAGTGATATACAGTCGCTAAAAACAGAATGCTATTGCCTTCTAGCGGaa                                                                                                                       >  2:372876/1‑140 (MQ=255)                 aTGAAATGTTAGCCGGAAAGAAAGAACTTTACGACTTCACCCCTGAAAGCATTTACAGGGCCATGACTATATTTGATAGATTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGaa                                                                                                                       <  2:282921/140‑1 (MQ=255)                 aTGAAATGTTAGCCGGAAAGAAAGAACTTTACGACTTCACCCCTGAAAGCATTTACAGGGCCATGACTATATTTGATAGATTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGaa                                                                                                                       <  1:384368/140‑1 (MQ=255)                  tGAAATGTTAGCCGGAAAGAAAGAACTTTACGACTTCACCCCTGAAAGCATTTACAGGGCCATGACTATATTTGATAGATTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAAt                                                                                                                      <  1:98081/140‑1 (MQ=255)                       tGTTAGCCGGAAAGAAAGAACTTTACGACTTCACCCCTGAAAGCATTTACAGGGCCATGACTATATTTGATAGATTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCat                                                                                                                 <  2:207461/140‑1 (MQ=255)                                 aaagaaagAACTTTACGACTTCACCCCTGAAAGCATTTACAGGGCCATGACTATATTTGATAGATTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTgg                                                                                                       >  2:339326/1‑140 (MQ=255)                                 aaagaaagAACTTTACGACTTCACCCCTGAAAGCATTTACAGGGCCATGACTATATTTGATAGATTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTgg                                                                                                       >  1:465730/1‑140 (MQ=255)                                 aaagaaagAACTTTACGACTTCACCCCTGAAAGCATTTACAGGGCCATGACTATATTTGATAGATTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGCTAATGCCTTCTAGCGGAATGCCATATGTCTTTgg                                                                                                       >  1:241353/1‑140 (MQ=255)                                 aaagaaagAACTTTACGACTTCACCCCTGAAAGCATTTACAGGGCCATGACTATATTTGATAGATTACAAAATAAAAGTGATAAACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTgg                                                                                                       >  2:45715/1‑140 (MQ=255)                                                       aCCCCTGAAAGCATTTACAGGGCCATGACTATATTTGATAGATTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGtgaact                                                                                 <  2:135576/140‑1 (MQ=255)                                                              aaaGCATTTACAGGGCCATGACTATATTTGATAGATTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTgaacttgaactt                                                                          >  2:36104/1‑140 (MQ=255)                                                              aaaGCATTTACAGGGCCATGACTATATTTGATAGATTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTgaacttgaactt                                                                          >  2:334225/1‑140 (MQ=255)                                                                        cAGGGCCATGACTATATTTGATAGATTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCaaa                                                                <  1:446284/140‑1 (MQ=255)                                                                         aGGGCCATGACTATATTTGATAGATTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCaaaa                                                               <  2:264590/140‑1 (MQ=255)                                                                          gggCCATGACTATATTTGATAGATTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCaaaaa                                                              >  2:324932/1‑140 (MQ=255)                                                                            gCCATGACTATATTTGATAGATTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGc                                                            >  1:505826/1‑140 (MQ=255)                                                                                 gACTATATTTGATAGATTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTag                                                       <  1:334225/140‑1 (MQ=255)                                                                                      tatTTGATAGATTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCtt                                                  <  2:505826/140‑1 (MQ=255)                                                                                      tatTTGATAGATTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCtt                                                  >  1:316830/1‑140 (MQ=255)                                                                                        tttGATAGATTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCtttt                                                >  1:252328/1‑140 (MQ=255)                                                                                        tttGATAGATTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCtttt                                                >  1:187754/1‑140 (MQ=255)                                                                                           gATAGATTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCTTTTAGa                                             >  2:332336/1‑140 (MQ=255)                                                                                            aTAGATTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCTTTTAGAt                                            >  1:119247/1‑140 (MQ=255)                                                                                             tAGATTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCTTTTAGAtt                                           <  1:50794/140‑1 (MQ=255)                                                                                             tAGATTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCTTTTAGAtt                                           <  1:36104/140‑1 (MQ=255)                                                                                                aTTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGCTATTGCCCTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTa                                                        <  2:431424/124‑1 (MQ=255)                                                                                                aTTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGCTATTGCCCTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTa                                                        >  1:431424/1‑124 (MQ=255)                                                                                                   aCAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCTTTTAGATTATGTAt                                     <  2:124541/140‑1 (MQ=255)                                                                                                     aaaaTAAAAGTGATATACAGACGCTAAAAACAGAATGCTAATGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCTTTTAGATTATGTATCa                                   <  2:241353/140‑1 (MQ=255)                                                                                                           aaaGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCTTTTAGATTATGTAt                                     <  1:38672/132‑1 (MQ=255)                                                                                                           aaaGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCTTTTAGATTATGTAt                                     <  1:5726/132‑1 (MQ=255)                                                                                                           aaaGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCTTTTAGATTATGTAt                                     >  2:5726/1‑132 (MQ=255)                                                                                                           aaaGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCTTTTAGATTATGTAt                                     >  2:38672/1‑132 (MQ=255)                                                                                                            aaGTGATATACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCTTTTAGATTATGTATCAGACATaa                            >  2:425791/1‑140 (MQ=255)                                                                                                                 atatACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCTTTTAGATTATGTATCAGACATAACCACt                       >  1:497127/1‑140 (MQ=255)                                                                                                                 atatACAGACGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCTTTTAGATTATGTATCAGACATAACCACt                       >  2:414601/1‑140 (MQ=255)                                                                                                                         aCGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCTTTTAGATTATGTATCAGACATAACCACTGTCGATgg               >  2:292463/1‑140 (MQ=255)                                                                                                                          cGCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCTTTTAGATTATGTATCAGACATAACCACTGTCGATGGa              <  1:425791/140‑1 (MQ=255)                                                                                                                           gCTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCTTTTAGATTATGTATCAGACATAACCACTGTCGATGGaa             >  2:494660/1‑140 (MQ=255)                                                                                                                            cTAAAAACAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCTTTTAGATTATGTATCAGACATAACCACTGTCGATGGaaa            >  2:14982/1‑140 (MQ=255)                                                                                                                              aaaaaCAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCTTTTAGATTATGTATCAGACATAACCACTGTCGATGGaaaaa          >  1:381213/1‑140 (MQ=255)                                                                                                                               aaaaCAGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCTTTTAGATTATGTATCAGACATAACCACTGTCGATGGAAAAAt         <  2:479850/140‑1 (MQ=255)                                                                                                                                    aGAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCTTTTAGATTATGTATCAGACATAACCACTGTCGATGGAAAAATTTTAg    <  1:414601/140‑1 (MQ=255)                                                                                                                                     gAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCTTTTAGATTATGTATCAGACATAACCACTGTCGATGGAAAAATTTTAGc   >  1:8512/1‑140 (MQ=255)                                                                                                                                     gAATGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCTTTTAGATTATGTATCAGACATAACCACTGTCGATGGAAAAATTTTAGc   <  2:497127/140‑1 (MQ=255)                                                                                                                                      aaTGCTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCTTTTAGATTATGTATCAGACATAACCACTGTCGATGGAAAAATTTTAGCt  >  2:307385/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        AATCATTATTTATCAGATGAAATGTTAGCCGGAAAGAAAGAACTTTACGACTTCACCCCTGAAAGCATTTACAGGGCCATGACTATATTTGATAGATTACAAAATAAAAGTGATATACAGACGCTAAAAACAGAATGTTATTGCCTTCTAGCGGAATGCCATATGTCTTTGGCACTTCATGGAAAAAGTGAACTTGAACTTGCTGCTCAAAAAGCATTAGAGCTTTTAGATTATGTATCAGACATAACCACTGTCGATGGAAAAATTTTAGCT  >  NC_000913/2992847‑2993119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑