Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,999,380	T→C	N171S (AAC→AGC)	ygeR ←	LysM domain‑containing putative peptidase lipoprotein YgeR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,999,380	0	T	C	100.0%	108.3 / NA	30	N171S (AAC→AGC)	ygeR	LysM domain‑containing putative peptidase lipoprotein YgeR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/10);  total (20/10)

CCCCATAGTGGCGATTTTTTGCCCAGCCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGTTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATA  >  NC_000913/2999244‑2999512                                                                                                                                         |                                                                                                                                     ccccGTAGTGGCGATTTTTTGCCCAGCCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGCTGc                                                                                                                                   >  2:315225/1‑140 (MQ=255)           gCGATTTTTTGCCCAGCCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGCTGCCCACATacac                                                                                                                         >  1:181431/1‑140 (MQ=255)                           ccTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGCTGCCCACATACACCACCTTTCCTGCAccc                                                                                                         <  2:147998/140‑1 (MQ=255)                                aCGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGCTGCCCACATACACCACCTTTCCTGCACCCgcggc                                                                                                    <  2:214954/140‑1 (MQ=255)                                     ttGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGCTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTaaa                                                                                               <  2:335903/140‑1 (MQ=255)                                                ttACCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGCTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTAAATAGGTGTAccc                                                                                    >  1:180261/1‑140 (MQ=255)                                                       cATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGCTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTaaa                                                                                               >  1:195492/1‑122 (MQ=255)                                                       cATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGCTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTaaa                                                                                               <  2:195492/122‑1 (MQ=255)                                                       cATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGCTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTAAATAGGTGTACCCCGTGGAg                                                                             >  2:127474/1‑140 (MQ=255)                                                         tCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGCTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTAAATAGGTGTACCCCGTGGAGCt                                                                           >  2:229361/1‑140 (MQ=255)                                                                      ggCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGCTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATcc                                                              >  1:399811/1‑140 (MQ=255)                                                                                 taatgtaatCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGCTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCTATCCCTTTATTgccg                                                   >  2:343551/1‑140 (MQ=255)                                                                                 taatgtaatCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGCTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATTgccg                                                   >  2:118673/1‑140 (MQ=255)                                                                                     gtaatCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGCTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCAt                                               <  2:180261/140‑1 (MQ=255)                                                                                       aatCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGCTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATct                                             >  2:88848/1‑140 (MQ=255)                                                                                        atCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGCTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATctg                                            >  1:259282/1‑140 (MQ=255)                                                                                             cACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGCTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTc                                       >  2:139464/1‑140 (MQ=255)                                                                                             cACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGCTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTc                                       >  2:231833/1‑140 (MQ=255)                                                                                                  tgtTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGCTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATctg                                            >  2:27547/1‑130 (MQ=255)                                                                                                  tgtTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGCTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATctg                                            <  1:27547/130‑1 (MQ=255)                                                                                                  tgtTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGCTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATa                                  <  2:399811/140‑1 (MQ=255)                                                                                                     ttAATCATGATGAGATTACCGTAGCCACGCAGCTGGCTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACgg                               >  2:410321/1‑140 (MQ=255)                                                                                                       aaTCATGATGAGATTACCGTAGCCACGCAGCTGGCTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCa                             <  1:229361/140‑1 (MQ=255)                                                                                                            tgatgaGATTACCGTAGCCACGCAGCTGGCTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATa                        >  2:72083/1‑140 (MQ=255)                                                                                                              atgaGATTACCGTAGCCACGCAGCTGGCTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAAc                      >  1:216417/1‑140 (MQ=255)                                                                                                                   aTTACCGTAGCCACGCAGCTGGCTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTcc                 >  1:238586/1‑140 (MQ=255)                                                                                                                     tACCGTAGCCACGCAGCTGGCTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCt               >  2:26455/1‑140 (MQ=255)                                                                                                                      aCCGTAGCCACGCAGCTGGCTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTg              >  1:510828/1‑140 (MQ=255)                                                                                                                          tAGCCACGCAGCTGGCTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGt          <  1:118673/140‑1 (MQ=255)                                                                                                                                  cAGCTGGCTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATa  <  1:315225/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     CCCCATAGTGGCGATTTTTTGCCCAGCCTTCACGCTTTGCCCATTATTTACCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAGCTGGTTGCCCACATACACCACCTTTCCTGCACCCGCGGCGTAAATAGGTGTACCCCGTGGAGCTGAGATATCAATCCCTTTATTGCCGCCATCTGCTGTCGAATACGGCATGATAACTTTCCCTGTCGTTGGCCATA  >  NC_000913/2999244‑2999512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑