Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,006,724	G→A	A155T (GCT→ACT)	ygeW →	putative carbamoyltransferase YgeW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,006,724	0	G	A	100.0%	99.9 / NA	30	A155T (GCT→ACT)	ygeW	putative carbamoyltransferase YgeW
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/15);  total (15/15)

CCTTCTGCGCCGACGCTATTGGTATTCGCGACGATATGTATCTGGGCGCAGGCAACGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTCAATGGCTGACCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCAAG  >  NC_000913/3006599‑3006860                                                                                                                              |                                                                                                                                         ccTTCTGCGCCGACGCTATTGGTATTCGCGACGATATGTATCTGGGCGCAGGCAACGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGACTTTAGTGAACCTg                                                                                                                            <  2:414011/140‑1 (MQ=255)     cTGCGCCGACGCTATTGGTATTCGCGACGATATGTATCTGGGCGCAGGCAACGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGACTTTAGTGAACCTGCAAt                                                                                                                        >  2:190858/1‑140 (MQ=255)     cTGCGCCGACGCTATTGGTATTCGCGACGATATGTATCTGGGCGCAGGCAACGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGACTTTAGTGAACCTGCAAt                                                                                                                        <  1:384455/140‑1 (MQ=255)      tGCGCCGACGCTATTGGTATTCGCGACGATATGTATCTGGGCGCAGGCAACGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGACTTTAGTGAACCTGCAATg                                                                                                                       <  2:448657/140‑1 (MQ=255)      tGCGCCGACGCTATTGGTAGTGGCGACGATATGTATCTGGGCGCAGGCAACGCGTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTGCAAGCAGGGTGTACTGCCACAGCGTCCGACTTTAGTGAACCTGCAATg                                                                                                                       <  1:522977/140‑1 (MQ=255)                        aTTCGCGACGATATGTATCTGGGCGCAGGCAACGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGACTTTAGTGAACCTGCAATGCGATATTGACCACCCGAc                                                                                                     >  1:106013/1‑140 (MQ=255)                         ttCGCGACGATATGTATCTGGGCGCAGGCAACGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGACTTTAGTGAACCTGCAATGCGATATTGACCACCCGACt                                                                                                    <  1:141596/140‑1 (MQ=255)                           cgcgACGATATGTATCTGGGCGCAGGCAACGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGACTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCa                                                                                                  >  2:384021/1‑140 (MQ=255)                                             ggCGCAGGCAACGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGACTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTCAATGGCTGACCTcgc                                                                                >  1:145975/1‑140 (MQ=255)                                               cgcAGGCAACGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGACTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTCAATGGCTGACCTcg                                                                                 >  2:289400/1‑137 (MQ=255)                                               cgcAGGCAACGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGACTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTCAATGGCTGACCTcg                                                                                 <  1:289400/137‑1 (MQ=255)                                                gcAGGCAACGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGACTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTCAATGGCTGACCTCGCGTg                                                                             >  1:438240/1‑140 (MQ=255)                                                 cAGGCAACGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGACTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTCAATGGCTGACCTcgc                                                                                >  2:176262/1‑136 (MQ=255)                                                 cAGGCAACGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGACTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTCAATGGCTGACCTcgc                                                                                <  1:176262/136‑1 (MQ=255)                                                   ggCAACGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGACTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTCAATGGCTGACCTCGCGTGGtt                                                                          >  1:372736/1‑140 (MQ=255)                                                   ggCAACGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGACTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTCAATGGCTGACCTCGCGTGGtt                                                                          >  1:108335/1‑140 (MQ=255)                                                          ccTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGACTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTCAATGGCTGACCTCGCGTGGTTACGTGaa                                                                   <  2:126986/140‑1 (MQ=255)                                                                   gTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGACTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTCAATGGCTGACCTCGCGTGGTTACGTGAACACTTTGGt                                                          >  2:121277/1‑140 (MQ=255)                                                                      aaGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGACTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTCAATGGCTGACCTCGCGTGGTTACGTGAACACTTTGGTTCa                                                       <  2:438240/140‑1 (MQ=255)                                                                                   aCTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGACTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTCAATGGCTGACCTCGCGTGGTTACGTGAACACTTTGGTTc                                                        >  1:386009/1‑126 (MQ=255)                                                                                   aCTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGACTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTCAATGGCTGACCTCGCGTGGTTACGTGAACACTTTGGTTc                                                        <  2:386009/126‑1 (MQ=255)                                                                                            cGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGACTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTCAATGGCTGACCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTaaaa                                 >  2:57501/1‑140 (MQ=255)                                                                                                    aGCAGGGTGTACTGCCACAGCGTCCGACTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTCAATGGCTGACCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGcc                         <  1:447683/140‑1 (MQ=255)                                                                                                         ggTGTACTGCCACAGCGTCCGACTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTCAATGGCTGACCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTa                                    <  2:415448/124‑1 (MQ=255)                                                                                                         ggTGTACTGCCACAGCGTCCGACTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTCAATGGCTGACCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTa                                    >  1:415448/1‑124 (MQ=255)                                                                                                         ggTGTACTGCCACAGCGTCCGACTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTCAATGGCTGACCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGAc                    >  1:519888/1‑140 (MQ=255)                                                                                                                  ccgcaGCGTCCGACTTTAGTGAACCTGCAATGCGATATTGACCTCCCGACTCAGTCAATGGCTGACCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAACCCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTa           <  2:184692/137‑1 (MQ=255)                                                                                                                  ccACAGCGTCCGACTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTCAATGGCTGACCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTa           <  1:57501/140‑1 (MQ=255)                                                                                                                   cacaGCGTCCGACTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTCAATGGCTGACCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCAt                       <  2:420206/127‑1 (MQ=255)                                                                                                                   cacaGCGTCCGACTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTCAATGGCTGACCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCAt                       >  1:420206/1‑127 (MQ=255)                                                                                                                           ccGACTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTCAATGGCTGACCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCAAg  <  2:326828/140‑1 (MQ=255)                                                                                                                              |                                                                                                                                         CCTTCTGCGCCGACGCTATTGGTATTCGCGACGATATGTATCTGGGCGCAGGCAACGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTCAATGGCTGACCTCGCGTGGTTACGTGAACACTTTGGTTCACTGGAAAACCTGAAAGGTAAAAAAATCGCCATGACCTGGGCCTACTCTCCAAG  >  NC_000913/3006599‑3006860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑