Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,042,241	A→G	D310G (GAC→GGC)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,042,241	0	A	G	100.0%	92.9 / NA	27	D310G (GAC→GGC)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/16);  total (11/16)

AGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTAT  >  NC_000913/3042107‑3042372                                                                                                                                       |                                                                                                                                    agagCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATg                                                                                                                                >  2:75714/1‑140 (MQ=255) agagCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATg                                                                                                                                >  2:262506/1‑140 (MQ=255) agagCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATg                                                                                                                                >  1:431712/1‑140 (MQ=255)    gCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGa                                                                                                                             <  2:206459/140‑1 (MQ=255)    gCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGa                                                                                                                             <  1:75714/140‑1 (MQ=255)         aaaTGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACa                                                                                                                        <  2:202736/140‑1 (MQ=255)          aaTGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACAt                                                                                                                       <  1:262506/140‑1 (MQ=255)                      cTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGc                                                                                                           <  2:431712/140‑1 (MQ=255)                      cTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGc                                                                                                           <  2:140842/140‑1 (MQ=255)                                  cggtacggtaCTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTAtt                                                                                               <  2:98147/140‑1 (MQ=255)                                          tacTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCTGATAGCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGaa                                                                                       >  2:9585/1‑140 (MQ=255)                                                cTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTaa                                                                                 <  2:112951/140‑1 (MQ=255)                                                  gCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAAt                                                                               <  1:533477/140‑1 (MQ=255)                                                   cGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAAtc                                                                              <  2:243468/140‑1 (MQ=255)                                                    ggTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTaa                                                                                 >  2:228333/1‑136 (MQ=255)                                                    ggTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTaa                                                                                 <  1:228333/136‑1 (MQ=255)                                                    ggTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAAtct                                                                             >  2:537236/1‑140 (MQ=255)                                                                   tGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAgg                                                              <  1:403213/140‑1 (MQ=255)                                                                                         gtcATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGc                                        >  1:191643/1‑140 (MQ=255)                                                                                                aTAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGcgcg                                 >  2:418730/1‑140 (MQ=255)                                                                                                     gATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTa                            >  2:155041/1‑140 (MQ=255)                                                                                                        atGGAACCGGATAGCATCTTCCGCGTACGCGGCGATACGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCa                         >  2:386358/1‑140 (MQ=255)                                                                                                                     gCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTg            <  1:537236/140‑1 (MQ=255)                                                                                                                       aTCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGAt          <  2:347906/140‑1 (MQ=255)                                                                                                                           tCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGcc       <  1:383077/139‑1 (MQ=255)                                                                                                                           tCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGcc       >  2:383077/1‑139 (MQ=255)                                                                                                                               cgTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTAt  <  2:388149/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                    AGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTAT  >  NC_000913/3042107‑3042372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑