Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,048,407	A→G	V212A (GTC→GCC)	gcvP ←	glycine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,048,407	0	A	G	100.0%	65.4 / NA	19	V212A (GTC→GCC)	gcvP	glycine decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/6);  total (13/6)

GCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGACCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCAAAGGTTTCGGCACGAGTACGGACCACATCCAGCGTTTGCGGATGCACATCGGAAGCCACGAAGAAGCGGTTGGCATTT  >  NC_000913/3048272‑3048538                                                                                                                                        |                                                                                                                                    gcAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGACCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGGCGTc                                                                                                                                 >  2:444561/1‑140 (MQ=255) gcAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGACCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAATCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGGCGTc                                                                                                                                 >  2:335451/1‑140 (MQ=255)     ttAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGACCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGGCGTCCTGa                                                                                                                             <  1:283589/140‑1 (MQ=255)                           atCGGCGGCAACGCTGACCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGGCGTCCTGATGGTCGAGCACTTTTTGcgcgc                                                                                                       <  1:444561/140‑2 (MQ=255)                               gcggcAACGCTGACCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGGCGTCCTGATGGTCGAGCACTTTTTGCGCGTCATc                                                                                                   >  2:151941/1‑140 (MQ=255)                                 ggcAACGCTGACCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGGCGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGa                                                                                                 >  2:130910/1‑140 (MQ=255)                                   cAACGCTGACCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGGCGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACa                                                                                               <  2:255430/140‑1 (MQ=255)                                            ccACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGGCGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCa                                                                                      <  1:349485/140‑1 (MQ=255)                                                              tCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGGCGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCAAAGGTTTCGGCa                                                                    <  2:387988/140‑1 (MQ=255)                                                                               gcAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGGCGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCAAAGGTTTCGGCACGAGTACGGACCACATc                                                   >  1:28008/1‑140 (MQ=255)                                                                                   tgtAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGGCGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCAAAGGTTTCGGCACGAGTACGGACCACATCCAGc                                               >  1:271158/1‑140 (MQ=255)                                                                                   tgtAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGGCGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCAAAGGTTTCGGCACGAGTACGGACCACATCCAGc                                               >  2:116069/1‑140 (MQ=255)                                                                                   tgtAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGGCGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCAAAGGTTTCGGCACGAGTACGGACCACATCCAGc                                               >  1:534805/1‑140 (MQ=255)                                                                                             aaTTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGGCGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCAAAGGTTTCGGCACGAGTACGGACCACATCCAGCGTTTGCGGAt                                     >  1:509356/1‑140 (MQ=255)                                                                                             aaTTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGGCGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCAAAGGTTTCGGCACGAGTACGGACCACATCCAGCGTTTGCGGAt                                     >  2:44061/1‑140 (MQ=255)                                                                                              aTTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGGCGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCAAAGGTTTCGGCACGAGTACGGACCACATCCAGCGTTTGCGGATg                                    <  2:28008/140‑1 (MQ=255)                                                                                                               tACCTGCTGTAACAGCACGCCGAAGGCGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCAAAGGTTTCGGCACGAGTACGGACCACATCCAGCGTTTGCGGATGCACATCGGAAGCCACga                   >  1:536450/1‑140 (MQ=255)                                                                                                                               aCGCCGAAGGCGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCAAAGGTTTCGGCACGAGTACGGACCACATCCAGCGTTTGCGGATGCACATCGGAAGCCACGAAGAAGCGGTTGGCAtt   >  1:196108/1‑140 (MQ=255)                                                                                                                                cGCCGAAGGCGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCAAAGGTTTCGGCACGAGTACGGACCACATCCAGCGTTTGCGGATGCACATCGGAAGCCACGAAGAAGCGGTTGGCAttt  >  1:441195/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                    GCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGACCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCAAAGGTTTCGGCACGAGTACGGACCACATCCAGCGTTTGCGGATGCACATCGGAAGCCACGAAGAAGCGGTTGGCATTT  >  NC_000913/3048272‑3048538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑