Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,052,056	T→C	S88G (AGC→GGC)	ubiI ←	2‑octaprenylphenol hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,052,056	0	T	C	100.0%	106.7 / NA	30	S88G (AGC→GGC)	ubiI	2‑octaprenylphenol hydroxylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/17);  total (13/17)

CGGGGGCTAACAGAGTGATATCTGACGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCTGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATT  >  NC_000913/3051921‑3052191                                                                                                                                        |                                                                                                                                        cGGGGGCTAACAGAGTGATATCTGACGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCt                                                                                                                                     <  2:251480/140‑1 (MQ=255)  gggggCTAACAGAGTGATATCTGACGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCtt                                                                                                                                    <  2:319739/140‑1 (MQ=255)       cTAACAGAGTGATATCTGACGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTcc                                                                                                                               <  1:290427/140‑1 (MQ=255)       cTAACAGAGTGATATCTGACGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTcc                                                                                                                               <  1:506518/140‑1 (MQ=255)         aaCAGAGTGATATCTGACGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCca                                                                                                                             <  2:82916/140‑1 (MQ=255)           cAGAGTGATATCTGACGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCcaca                                                                                                                           <  1:492300/140‑1 (MQ=255)                tGATATCTGACGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCca                                                                                                                             >  1:239962/1‑133 (MQ=255)                tGATATCTGACGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCca                                                                                                                             <  2:239962/133‑1 (MQ=255)                tGATATCTGACGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTcc                                                                                                                      >  2:439301/1‑140 (MQ=255)                            acTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATaa                                                                                                          >  1:47741/1‑140 (MQ=255)                                aTGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGc                                                                                                      >  1:441845/1‑140 (MQ=255)                                     cTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGcc                                                                                                 >  2:97309/1‑140 (MQ=255)                                        tGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTa                                                                                              <  1:97309/140‑1 (MQ=255)                                        tGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTa                                                                                              >  1:486208/1‑140 (MQ=255)                                          ttCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACg                                                                                            >  2:332003/1‑140 (MQ=255)                                               caGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACgagaga                                                                                       <  2:486208/140‑1 (MQ=255)                                                  cgcgTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGaa                                                                                    >  1:308874/1‑140 (MQ=255)                                                  cgcgTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGaa                                                                                    >  2:55682/1‑140 (MQ=255)                                                            aTCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCa                                                                          <  2:257653/140‑1 (MQ=255)                                                            aTCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCa                                                                          <  1:439301/140‑1 (MQ=255)                                                               aCTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGAc                                                                       <  2:417114/140‑1 (MQ=255)                                                                 tGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGc                                                                     >  2:225830/1‑140 (MQ=255)                                                                           aCGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGt                                                           <  1:55682/140‑1 (MQ=255)                                                                              atatGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAg                                                        <  2:308874/140‑1 (MQ=255)                                                                                           ggCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTg                                           <  1:332003/140‑1 (MQ=255)                                                                                               ataGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTggcgg                                       <  2:441845/140‑1 (MQ=255)                                                                                                taGCCCATGCTTTGATCGTCAAACGAAATGGGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCGCTACGAGAGAGAATGCCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTggcggc                                      >  2:499639/1‑140 (MQ=255)                                                                                                                   cAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACgcgc                   >  2:247404/1‑140 (MQ=255)                                                                                                                               gACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTgtggtggt       >  2:228180/1‑140 (MQ=255)                                                                                                                                    aaGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCAtt  >  2:362738/1‑140 (MQ=255)                                                                                                                                    aaGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCAtt  <  1:247404/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        CGGGGGCTAACAGAGTGATATCTGACGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCTGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATT  >  NC_000913/3051921‑3052191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑