Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,052,577	A→C	T314T (ACT→ACG)	ubiH ←	2‑octaprenyl‑6‑methoxyphenol hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,052,577	0	A	C	100.0%	105.7 / NA	30	T314T (ACT→ACG)	ubiH	2‑octaprenyl‑6‑methoxyphenol hydroxylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (16/14);  total (16/14)

GGTGCCCAACGGTTGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTA  >  NC_000913/3052440‑3052710                                                                                                                                          |                                                                                                                                      ggTGCCCAACGGTTGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGt                                                                                                                                     >  1:498657/1‑140 (MQ=255)  gTGCCCAACGGTTGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTc                                                                                                                                    <  1:88550/140‑1 (MQ=255)      ccAACAGTTGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAggg                                                                                                                                >  1:364438/1‑140 (MQ=255)       cAACGGTTGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGt                                                                                                                               <  2:498657/140‑1 (MQ=255)             ttGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGc                                                                                                                         >  2:143570/1‑140 (MQ=255)              tGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCa                                                                                                                        <  2:133798/140‑1 (MQ=255)                         tGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATcac                                                                                                             >  2:210964/1‑140 (MQ=255)                          gTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATcaca                                                                                                            >  1:202360/1‑140 (MQ=255)                                     tCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATAcc                                                                                                 <  1:111266/140‑1 (MQ=255)                                     tCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATAcc                                                                                                 >  2:111266/1‑140 (MQ=255)                                     tCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATAcc                                                                                                 >  1:477144/1‑140 (MQ=255)                                            cGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTa                                                                                          <  2:364438/140‑1 (MQ=255)                                                 aTGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAAccc                                                                                     >  2:392141/1‑140 (MQ=255)                                                      tGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGcc                                                                                >  1:439901/1‑140 (MQ=255)                                                               aTCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCg                                                                       <  2:439901/140‑1 (MQ=255)                                                                                      aaCGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTg                                                <  1:392141/140‑1 (MQ=255)                                                                                           cacaATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCccac                                           >  2:505632/1‑140 (MQ=255)                                                                                           cacaATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCccac                                           >  2:225792/1‑140 (MQ=255)                                                                                                     cGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTa                                 >  1:408618/1‑140 (MQ=255)                                                                                                     cGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTa                                 >  1:108061/1‑140 (MQ=255)                                                                                                       tAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTACg                               <  1:143570/140‑1 (MQ=255)                                                                                                       tAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTACg                               <  2:202360/140‑1 (MQ=255)                                                                                                       tAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTACg                               <  1:505632/140‑1 (MQ=255)                                                                                                        aaTCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTACGa                              <  1:225792/140‑1 (MQ=255)                                                                                                        aaTCCCCCATGTCTTCTCCGCGCTCCTGCGCCCGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTACAGAGTTTGCGCCGCATTGCCCACCAGCACGGTACGa                              <  2:477144/140‑1 (MQ=255)                                                                                                                     ttctCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATc                 >  1:358131/1‑140 (MQ=255)                                                                                                                        tcCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTACGAt                             <  2:213746/125‑1 (MQ=255)                                                                                                                        tcCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTACGAt                             >  1:213746/1‑125 (MQ=255)                                                                                                                             gcTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGgcggcg         <  2:408618/140‑1 (MQ=255)                                                                                                                                    cgcCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTa  >  1:144907/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      GGTGCCCAACGGTTGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTA  >  NC_000913/3052440‑3052710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑