Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	234,812	T→C	intergenic (‑30/‑4)	gloB ← / → yafS	hydroxyacylglutathione hydrolase GloB/putative S‑adenosyl‑L‑methionine‑dependent methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	234,812	0	T	C	100.0%	128.0 / NA	36	intergenic (‑30/‑4)	gloB/yafS	hydroxyacylglutathione hydrolase GloB/putative S‑adenosyl‑L‑methionine‑dependent methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/17);  total (19/17)

TGGCTCTGCGTCTCCGGGATCGACAATCAGGCAGCGACCTGCTTCATCATTCAAAACCCAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCAT  >  NC_000913/234681‑234947                                                                                                                                    |                                                                                                                                        tGGCTCTGCGTCTCCGGGATCGACAATCAGGCAGCGACCTGCTTCATCATTCAAAACCCAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTCGTGATGaa                                                                                                                                 <  2:228354/140‑1 (MQ=255) tGGCTCTGCGTCTCCGGGATCGACAATCAGGCAGCGACCTGCTTCATCATTCAAAACCCAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTCGTGATGaa                                                                                                                                 >  2:512250/1‑140 (MQ=255) tGGCTCTGCGTCTCCGGGATCGACAATCAGGCAGCGACCTGCTTCATCATTCAAAACCCAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTCGTGATGaa                                                                                                                                 >  2:474318/1‑140 (MQ=255)   gCTCTGCGTCTCCGGGATCGACAATCAGGCAGCGACCTGCTTCATCATTCAAAACCCAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTCGTGATGAAAc                                                                                                                               >  1:386346/1‑140 (MQ=255)      ctGCGTCTCCGGGATCGACAATCAGGCAGCGACCTGCTTCATCATTCAAAACCCAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTCGTGATGAAACCgg                                                                                                                            >  1:376824/1‑140 (MQ=255)       tGCGTCTCCGGGATCGACAATCAGGCAGCGACCTGCTTCATCATTCAAAACCCAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTCGTGATGAAACCGGc                                                                                                                           >  1:13527/1‑140 (MQ=255)        gCGTCTCCGGGATCGACAATCAGGCAGCGACCTGCTTCATCATTCAAAACCCAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTCGTGATGAAACCGGCa                                                                                                                          >  1:485778/1‑140 (MQ=255)         cGTCTCCGGGATCGACAATCAGGCAGCGACCTGCTTCATCATTCAAAACCCAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTCGTGATGAAAc                                                                                                                               >  2:510184/1‑134 (MQ=255)         cGTCTCCGGGATCGACAATCAGGCAGCGACCTGCTTCATCATTCAAAACCCAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTCGTGATGAAAc                                                                                                                               <  1:510184/134‑1 (MQ=255)           tctcCGGGATCGACAATCAGGCAGCGACCTGCTTCATCATTCAAAACCCAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTCGTGATGAAACCGGCAaga                                                                                                                       <  2:386346/140‑1 (MQ=255)             tcCGGGATCGACAATCAGGCAGCGACCTGCTTCATCATTCAAAACCCAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTCGTGATGAAACCGGCAAGAGt                                                                                                                     <  1:408239/140‑1 (MQ=255)                 ggATCGACAATCAGGCAGCGACCTGCTTCATCATTCAAAACCCAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTCGTGATGAAACCGGCAAGAGTCCCt                                                                                                                 >  2:411829/1‑140 (MQ=255)                           tCAGGCAGCGACCTGCTTCATCATTCAAAACCCAGATGTAATTGTCATCAAAGGCGGGAATACTGTTATGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTCGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCg                                                                                                       <  2:376824/140‑1 (MQ=255)                                   cGACCTGCTTCATCATTCAAAACCCAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTCGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCt                                                                                               >  1:85867/1‑140 (MQ=255)                                     aCCTGCTTCATCATTCAAAACCCAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTCGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTc                                                                                                 <  1:122329/136‑1 (MQ=255)                                     aCCTGCTTCATCATTCAAAACCCAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTCGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTc                                                                                                 >  2:122329/1‑136 (MQ=255)                                                  ttCAAAACCCAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTCGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGAt                                                                                <  2:485778/140‑1 (MQ=255)                                                             gATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTCGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTgggg                                                                     >  1:436487/1‑140 (MQ=255)                                                               tGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTCGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGaa                                                                   <  2:85867/140‑1 (MQ=255)                                                                         atcaAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTCGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCg                                                         >  1:241756/1‑140 (MQ=255)                                                                              aGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTCGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAgg                                                    <  1:411829/140‑1 (MQ=255)                                                                                         cTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTCGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAgcgc                                         <  2:5362/140‑1 (MQ=255)                                                                                          tGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTCGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGcc                                        <  1:133825/140‑1 (MQ=255)                                                                                               aGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTCGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTc                                   >  2:418195/1‑140 (MQ=255)                                                                                                 aTTCATAGATTACCTCTCAGTGTGAAACGGAAGGTCGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCaa                                 >  1:202398/1‑140 (MQ=255)                                                                                                  ttCATAGATTACCTCTCAGTGTGAAACGGAAGGTCGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAAc                                <  2:436487/140‑1 (MQ=255)                                                                                                    cATAGATTACCTCTCAGTGTGAAACGGAAGGTCGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAAccc                              >  1:183876/1‑140 (MQ=255)                                                                                                       aGATTACCTCTCAGTGTGAAACGGAAGGTCGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTg                           <  2:202398/140‑1 (MQ=255)                                                                                                            aCCTCTCAGTGTGAAACGGAAGGTCGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCa                      >  2:271518/1‑140 (MQ=255)                                                                                                                   aGTGTGAAACGGAAGGTCGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGtt                        >  2:217327/1‑131 (MQ=255)                                                                                                                   aGTGTGAAACGGAAGGTCGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGtt                        <  1:217327/131‑1 (MQ=255)                                                                                                                   aGTGTGAAACGGAAGGTCGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAAt               >  1:58307/1‑140 (MQ=255)                                                                                                                   aGTGTGAAACGGAAGGTCGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAAt               >  1:234765/1‑140 (MQ=255)                                                                                                                               aaGGTCGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCa   <  1:68829/140‑1 (MQ=255)                                                                                                                                aGGTCGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCAt  <  1:512250/140‑1 (MQ=255)                                                                                                                                aGGTCGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCAt  <  1:474318/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        TGGCTCTGCGTCTCCGGGATCGACAATCAGGCAGCGACCTGCTTCATCATTCAAAACCCAGATGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCAT  >  NC_000913/234681‑234947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑