Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,181,423	G→A	V335I (GTT→ATT)	ygiC →	putative enzyme with ATPase activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,181,423	0	G	A	100.0%	156.1 / NA	45	V335I (GTT→ATT)	ygiC	putative enzyme with ATPase activity
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/21);  total (24/21)

CGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGAT  >  NC_000913/3181286‑3181545                                                                                                                                          |                                                                                                                           cGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCA‑CGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTc                                                                                                                         >  1:75953/1‑140 (MQ=255)   gAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCa                                                                                                                        >  2:234390/1‑140 (MQ=255)              tcCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCAcc                                                                                                             >  2:42605/1‑140 (MQ=255)                cGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCg                                                                                                           >  1:183173/1‑140 (MQ=255)                cGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCg                                                                                                           >  1:527747/1‑140 (MQ=255)                 gCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGt                                                                                                          >  2:115107/1‑140 (MQ=255)                               atGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAAt                                                                                                 >  2:298682/1‑135 (MQ=255)                               atGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAAt                                                                                                 <  1:298682/135‑1 (MQ=255)                                tgtgGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCg                                                                                           <  1:129881/140‑1 (MQ=255)                                             aTCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGa                                                                                          <  1:127688/128‑1 (MQ=255)                                             aTCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGa                                                                                          >  2:127688/1‑128 (MQ=255)                                             aTCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGAGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCt                                                                              >  1:430988/1‑140 (MQ=255)                                               cttctCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGa                                                                            >  1:68369/1‑140 (MQ=255)                                               cttctCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGa                                                                            >  1:428425/1‑140 (MQ=255)                                                ttctCCCGTGAAGGCGCAGACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGAt                                                                           <  2:93305/140‑1 (MQ=255)                                                  ctcCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTg                                                                         >  1:427344/1‑140 (MQ=255)                                                   tcCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTgg                                                                        <  1:395062/140‑1 (MQ=255)                                                       gTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAgc                                                                    <  1:293322/140‑1 (MQ=255)                                                          aaGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAgctgg                                                                 >  2:358566/1‑140 (MQ=255)                                                           aGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAgctggc                                                                <  1:42605/140‑1 (MQ=255)                                                            ggCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAgctggct                                                               >  2:327855/1‑140 (MQ=255)                                                            ggCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAgctggct                                                               >  1:48487/1‑140 (MQ=255)                                                                                  agaACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTGCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGa                                         >  2:48027/1‑140 (MQ=255)                                                                                  agaACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGa                                         >  2:85461/1‑140 (MQ=255)                                                                                    aaCGGCAAAACCGTTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAAt                                       <  2:532428/140‑1 (MQ=255)                                                                                    aaCGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAAt                                       <  2:68369/140‑1 (MQ=255)                                                                                      cGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTg                                     >  2:211428/1‑140 (MQ=255)                                                                                      cGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTg                                     >  2:384268/1‑140 (MQ=255)                                                                                       ggCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTgg                                    <  2:48487/140‑1 (MQ=255)                                                                                       ggCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTgg                                    >  2:446520/1‑140 (MQ=255)                                                                                         cAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCa                                  <  1:327855/140‑1 (MQ=255)                                                                                         cAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCa                                  <  1:211428/140‑1 (MQ=255)                                                                                           aaaCCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCAtt                                <  1:358566/140‑1 (MQ=255)                                                                                               cATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTg                            <  1:234390/140‑1 (MQ=255)                                                                                                 ttGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCa                                  <  1:106393/132‑1 (MQ=255)                                                                                                 ttGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCa                                  >  2:106393/1‑132 (MQ=255)                                                                                                   gAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGa                        <  1:446520/140‑1 (MQ=255)                                                                                                        agcGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTg                   >  2:57872/1‑140 (MQ=255)                                                                                                        agcGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTg                   <  1:384268/140‑1 (MQ=255)                                                                                                         gcGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGc                  >  1:185959/1‑140 (MQ=255)                                                                                                          cGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCAACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCa                 >  1:411919/1‑140 (MQ=255)                                                                                                               ggTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCAACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGAt            <  2:411919/140‑1 (MQ=255)                                                                                                                  ccGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCAc         <  2:428425/140‑1 (MQ=255)                                                                                                                   cGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCAcc        <  1:115107/140‑1 (MQ=255)                                                                                                                         gCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATTACCCAGGat  <  2:75953/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                           CGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGAT  >  NC_000913/3181286‑3181545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑