Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,198,913	T→C	E243E (GAA→GAG)	glnE ←	fused glutamine synthetase deadenylase/glutamine synthetase adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,198,913	0	T	C	100.0%	66.2 / NA	19	E243E (GAA→GAG)	glnE	fused glutamine synthetase deadenylase/glutamine synthetase adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/9);  total (10/9)

CGACGGAAAACAAACGGGCGCAGCATCGCACGCAACTCGTTAGCATAGACGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGGTTGATCCAG  >  NC_000913/3198785‑3199014                                                                                                                                 |                                                                                                      cgacgGAAAACAAACGGGCGCAGCATCGCACGCAACTCGTTAGCATAGACGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCCTCCAACGCGGc                                                                                            >  2:311341/1‑140 (MQ=255)  gacgGAAAACAAACGGGCGCAGCATCGCACGCAACTCGTTAGCATAGACGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCCTCCAACGCGGCa                                                                                           <  2:275863/140‑1 (MQ=255)              acGGGCGCAGCATCGCACGCAACTTGTTAGCATAGACGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCCTCCAACGCGGCAAAGCTCAGCAcc                                                                               >  2:72348/1‑140 (MQ=255)                                 caACTCGTTAGCATAGACGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCCTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCaa                                                            <  1:201970/140‑1 (MQ=255)                                        ttAGCATAGACGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCCTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACg                                                     <  1:266217/140‑1 (MQ=255)                                        ttAGCATAGACGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCCTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACg                                                     <  1:311341/140‑1 (MQ=255)                                                 aCGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCCTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCat                                            >  1:148986/1‑140 (MQ=255)                                                 aCGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCCTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCat                                            >  1:442332/1‑140 (MQ=255)                                                 aCGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCCTCCAACGCGGCAAAGCTCAGCACCAGCGGCCCACTTTCGCCAAACGGACGCAGCCGCat                                            >  2:143919/1‑140 (MQ=255)                                                           tATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCCTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGa                                  <  2:322708/140‑1 (MQ=255)                                                                     aaTCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCCTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACg                            >  1:533609/1‑136 (MQ=255)                                                                     aaTCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCCTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACg                            <  2:533609/136‑1 (MQ=255)                                                                           cgcCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCCTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCt                  >  1:50019/1‑140 (MQ=255)                                                                           cgcCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCCTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCt                  >  1:298170/1‑140 (MQ=255)                                                                                tGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCCTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCgtt             >  2:360025/1‑140 (MQ=255)                                                                                   ccATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCCTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCgttggt          <  2:442332/140‑1 (MQ=255)                                                                                      tCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCCTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAg                         <  2:146796/122‑1 (MQ=255)                                                                                      tCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCCTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAg                         >  1:146796/1‑122 (MQ=255)                                                                                           tAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCCTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGGTTGATCCAg  <  1:360025/140‑1 (MQ=255)                                                                                                                                 |                                                                                                      CGACGGAAAACAAACGGGCGCAGCATCGCACGCAACTCGTTAGCATAGACGCCTTCGCTATCGCCCATAATCCGCGCCTTGACCATCGCGTAACGCTCCCAGTCGCGCCCCTGCTCCTGGTAATAATCTTCCAACGCGGCAAAGCTCAGCACCAGCGGGCCACTTTCGCCAAACGGACGCAGCCGCATATCCACGCGATAGACGAAGCCATCCTGCGTTGGTTGATCCAG  >  NC_000913/3198785‑3199014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑