Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,235,383	T→C	T165A (ACG→GCG)	rlmG ←	23S rRNA m(2)G1835 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,235,383	0	T	C	100.0%	143.6 / NA	40	T165A (ACG→GCG)	rlmG	23S rRNA m(2)G1835 methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/17);  total (23/17)

CGGGAGAAGACATTCGCATGGTTGTGGATAGTCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAA  >  NC_000913/3235247‑3235518                                                                                                                                         |                                                                                                                                        cGGGAGAAGACATTCGCATGGTTGTGGATAGTCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCgcggt                                                                                                                                      <  2:475815/140‑1 (MQ=255)         gaCATTCGCATGGTTGTGGATAGTCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTg                                                                                                                              >  1:139005/1‑140 (MQ=255)         gaCATTCGCATGGTTGTGGATAGTCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTg                                                                                                                              >  1:53525/1‑140 (MQ=255)              tCGCATGGTTGTGGATAGTCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCg                                                                                                                         >  2:140336/1‑140 (MQ=255)                            aTAGTCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGaa                                                                                                           <  2:113142/140‑1 (MQ=255)                                tCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCCATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGGGGTGGTTGGGCCGAGCACTTTTTCGAACAGt                                                                                                       >  2:228949/1‑140 (MQ=255)                                tCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGaa                                                                                                           >  1:8721/1‑136 (MQ=255)                                tCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGaa                                                                                                           <  2:8721/136‑1 (MQ=255)                                tCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGt                                                                                                       >  2:381265/1‑140 (MQ=255)                                tCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGt                                                                                                       >  2:18529/1‑140 (MQ=255)                                tCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGt                                                                                                       >  2:523473/1‑140 (MQ=255)                                tCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGt                                                                                                       >  1:531505/1‑140 (MQ=255)                                tCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGt                                                                                                       >  1:210578/1‑140 (MQ=255)                                tCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGt                                                                                                       >  1:148450/1‑140 (MQ=255)                                tCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGt                                                                                                       >  1:101735/1‑140 (MQ=255)                                  cagtcagtACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTc                                                                                                     <  2:446416/140‑1 (MQ=255)                                  cagtcagtACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTc                                                                                                     <  1:140336/140‑1 (MQ=255)                                  cagtcagtACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTc                                                                                                     <  2:210578/140‑1 (MQ=255)                                        gtACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCactgt                                                                                               <  1:381265/140‑5 (MQ=255)                                         tACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTg                                                                                              >  1:248806/1‑140 (MQ=255)                                             ttccagcttccagcTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAg                                                                                          >  2:24642/1‑140 (MQ=255)                                                                    gcggcgCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTT‑CATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCAc                                                                  >  2:332248/1‑140 (MQ=255)                                                                     cggcgCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCAc                                                                  <  1:320327/140‑1 (MQ=255)                                                                       gcgcATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCg                                                                >  2:141848/1‑140 (MQ=255)                                                                        cgcATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCgg                                                               <  1:141848/140‑1 (MQ=255)                                                                          cATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCa                                                             >  2:437052/1‑140 (MQ=255)                                                                                  aGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACg                                                     <  2:53525/140‑1 (MQ=255)                                                                                  aGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACg                                                     <  2:531505/140‑1 (MQ=255)                                                                                               tGAAAGTGCAATTAATCAGGCGCGCTTTTTT‑CATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGa                                       <  1:332248/140‑1 (MQ=255)                                                                                                gAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGa                                       <  2:248806/140‑1 (MQ=255)                                                                                                 aaaGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGAc                                      >  1:190694/1‑140 (MQ=255)                                                                                                    gTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCAc                                   >  1:121327/1‑140 (MQ=255)                                                                                                          ttAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTgcg                             <  2:139005/140‑1 (MQ=255)                                                                                                               cAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTgcgcagcg                        >  2:462445/1‑140 (MQ=255)                                                                                                               cAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTgcgcagcg                        >  1:260961/1‑140 (MQ=255)                                                                                                                 ggCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTgcgcagcgca                      >  1:431441/1‑140 (MQ=255)                                                                                                                  gcgcgcTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGcgcac                     <  2:34378/140‑1 (MQ=255)                                                                                                                          ttttCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGc             <  2:260961/140‑1 (MQ=255)                                                                                                                          ttttCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGc             <  2:101735/140‑1 (MQ=255)                                                                                                                                  gCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAg     >  2:538758/1‑140 (MQ=255)                                                                                                                                     aGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCaa  <  1:462445/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CGGGAGAAGACATTCGCATGGTTGTGGATAGTCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAA  >  NC_000913/3235247‑3235518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑