Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,259,315	T→C	N112S (AAC→AGC)	tdcG ←	L‑serine deaminase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,259,315	0	T	C	100.0%	136.6 / NA	38	N112S (AAC→AGC)	tdcG	L‑serine deaminase III
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/18);  total (20/18)

CTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGT  >  NC_000913/3259187‑3259443                                                                                                                                 |                                                                                                                                 cttcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACa                                                                                                                       >  1:284531/1‑140 (MQ=255)                aCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAata                                                                                                        >  2:333773/1‑140 (MQ=255)                aCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAata                                                                                                        <  2:39075/140‑1 (MQ=255)                                         tttACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAgcc                                                                               >  1:430045/1‑140 (MQ=255)                                         tttACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAgcc                                                                               >  2:158777/1‑140 (MQ=255)                                         tttACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAgcc                                                                               >  1:145844/1‑140 (MQ=255)                                           tACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAgccgc                                                                             >  1:13587/1‑140 (MQ=255)                                              ttAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGccgcccg                                                                          <  2:284531/140‑1 (MQ=255)                                                 aTAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTg                                                                       >  1:514162/1‑140 (MQ=255)                                                   aGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTgcg                                                                     <  2:417326/140‑1 (MQ=255)                                                    gCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTgcgc                                                                    <  2:185980/140‑1 (MQ=255)                                                       ctTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTgcgc                                                                    <  2:465498/137‑1 (MQ=255)                                                       ctTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTgcgc                                                                    >  1:465498/1‑137 (MQ=255)                                                          ccTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAgccg                                                                              <  2:380706/124‑1 (MQ=255)                                                          ccTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAgccg                                                                              >  1:380706/1‑124 (MQ=255)                                                          ccTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACt                                                              <  1:111960/140‑1 (MQ=255)                                                          ccTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACt                                                              <  1:333773/140‑1 (MQ=255)                                                          ccTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACt                                                              >  1:390436/1‑140 (MQ=255)                                                          ccTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACt                                                              <  1:192725/140‑1 (MQ=255)                                                           cTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTa                                                             <  2:236128/140‑1 (MQ=255)                                                            tGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTaa                                                            <  2:13587/140‑1 (MQ=255)                                                             gTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAAc                                                           <  1:158777/140‑1 (MQ=255)                                                                   tCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCtata                                                     >  1:136482/1‑140 (MQ=255)                                                                    ccAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACt                                                              <  1:95266/130‑1 (MQ=255)                                                                    ccAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACt                                                              >  2:95266/1‑130 (MQ=255)                                                                    ccAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATaa                                                    >  2:182562/1‑140 (MQ=255)                                                                                        cGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATg                                >  1:501060/1‑140 (MQ=255)                                                                                          ttCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGac                              >  2:29349/1‑140 (MQ=255)                                                                                              cATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGacaaca                          >  2:265768/1‑140 (MQ=255)                                                                                               aTGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACAt                         >  2:32304/1‑140 (MQ=255)                                                                                                   cGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTg                     <  1:11844/140‑1 (MQ=255)                                                                                                        cAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGAc                <  1:182562/140‑1 (MQ=255)                                                                                                         aaCATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGc                    <  2:234621/135‑1 (MQ=255)                                                                                                         aaCATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGc                    >  1:234621/1‑135 (MQ=255)                                                                                                             tttCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGttt           <  1:29349/140‑1 (MQ=255)                                                                                                                cGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCt        >  2:222928/1‑140 (MQ=255)                                                                                                                    aTGGAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCa    <  1:32304/140‑1 (MQ=255)                                                                                                                      ggAAGATAATGCTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGt  >  2:112033/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                 CTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGT  >  NC_000913/3259187‑3259443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑