Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,291,948	A→G	D203G (GAC→GGC)	yraK →	putative fimbrial adhesin YraK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,291,948	0	A	G	100.0%	106.9 / NA	30	D203G (GAC→GGC)	yraK	putative fimbrial adhesin YraK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/14);  total (16/14)

TTAATATTGGTGCGAACACCACCTTTACCCTGTATGTCACCAAGCCATTTCTCGGCTCGATGACCATTCCGAAAACGGATATTGCCGTCATTAAAGGCGCGTGGGTCGATGGAATGGGAAGCCCGTCTACAGGTGACTTCCATGATTTAGTCAAGTTATCGATTCAGGGAAATCTCACCGCCCCACAGTCGTGCAAAATTAATCAGGGCGATGTTATTAAGGTTAATTTTGGATTCATCAATGGTCAGAAGTTTACCACCCGCAATGCC  >  NC_000913/3291813‑3292081                                                                                                                                        |                                                                                                                                      ttAATATTGGTGCGAACACCACCTTTACCCTGTATGTCACCAAGCCATTTCTCGGCTCGATGACCATTCCGAAAACGGATATTGCCGTCATTAAAGGCGCGTGGGTCGATGGAATGGGAAGCCCGTCTACAGGTGGCTTc                                                                                                                                   >  2:227019/1‑140 (MQ=255)      atTGGTGCGAACACCACCTTTACCCTGTATGTCACCAAGCCATTTCTCGGCTCGATGACCATTCCGAAAACGGATATTGCCGTCATTAAAGGCGCGTGGGTCGATGGAATGGGAAGCCCGTCTACAGGTGGCTTCCATGa                                                                                                                              >  1:56592/1‑140 (MQ=255)          gTGCGAACACCACCTTTACCCTGTATGTCACCAAGCCATTTCTCGGCTCGATGACCATTCCGAAAACGGATATTGCCGTCATTAAAGGCGCGTGGGTCGATGGAATGGGAAGCCCGTCTACAGGTGGCTTCCATGATTTa                                                                                                                          >  2:461257/1‑140 (MQ=255)               aaCACCACCTTTACCCTGTATGTCACCAAGCCATTTCTCGGCTCGATGACCATTCCGAAAACGGATATTGCCGTCATTAAAGGCGCGTGGGTCGATGGAATGGGAAGCCCGTCTACAGGTGGCTTCCATGATTTAGTCaa                                                                                                                     <  2:210866/140‑1 (MQ=255)                    caccTTTACCCTGTATGTCACCAAGCCATTTCTCGGCTCGATGACCATTCCGAAAACGGATATTGCCGTCATTAAAGGCGCGTGGGTCGATGGAATGGGAAGCCCGTCTACAGGTGGCTTCCATGATTTAGTCAAGTTAt                                                                                                                >  2:117014/1‑140 (MQ=255)                              cTGTATGTCACCAAGCCATTTCTCGGCTCGATGACCATTCCGAAAACGGATATTGCCGTCATTAAAGGCGCGTGGGTCGATGGAATGGGAAGCCCGTCTACAGGTGGCTTCCATGATTTAGTCAAGTTATCGATTCAggg                                                                                                      >  1:507168/1‑140 (MQ=255)                                   tGTCACCAAGCCATTTCTCGGCTCGATGACCATTCCGAAAACGGATATTGCCGTCATTAAAGGCGCGTGGGTCGATGGAATGGGAAGCCCGTCTACAGGTGGCTTCCATGATTTAGTCAAGTTATCGATTCAGGGAAAtc                                                                                                 >  1:381604/1‑140 (MQ=255)                                       aCCAAGCCATTTCTCGGCTCGATGACCATTCCGAAAACGGATATTGCCGTCATTAAAGGCGCGTGGGTCGATGGAATGGGAAGCCCGTCTACAGGTGGCTTCCATGATTTAGTCAAGTTATCGATTCAGGGAAATCTCAc                                                                                             <  1:117014/140‑1 (MQ=255)                                       aCCAAGCCATTTCTCGGCTCGATGACCATTCCGAAAACGGATATTGCCGTCATTAAAGGCGCGTGGGTCGATGGAATGGGAAGCCCGTCTACAGGTGGCTTCCATGATTTAGTCAAGTTATCGATTCAGGGAAATCTCAc                                                                                             <  1:227019/140‑1 (MQ=255)                                       aCCAAGCCATTTCTCGGCTCGATGACCATTCCGAAAACGGATATTGCCGTCATTAAAGGCGCGTGGGTCGATGGAATGGGAAGCCCGTCTACAGGTGGCTTCCATGATTTAGTCAAGTTATCGATTCAGGGAAATCTCAc                                                                                             <  2:381604/140‑1 (MQ=255)                                           aGCCATTTCTCGGCTCGATGACCATTCCGAAAACGGATATTGCCGTCATTAAAGGCGCGTGGGTCGATGGAATGGGAAGCCCGTCTACAGGTGGCTTCCATGATTTAGTCAAGTTATCGATTCAGGGAAATCTCACCGcc                                                                                         >  1:302476/1‑140 (MQ=255)                                                        cTCGATGACCATTCCGAAAACGGATATTGCCGTCATTAAAGGCGCGTGGGTCGATGGAATGGGAAGCCCGTCTACAGGTGGCTTCCATGATTTAGTCAAGTTATCGATTCAGGGAAATCTCACCGCCCCACAGTCGTGCa                                                                            <  2:507168/140‑1 (MQ=255)                                                                     ccGAAAACGGATATTGCCGTCATTAAAGGCGCGTGGGTCGATGGAATGGGAAGCCCGTCTACAGGTGGCTTCCATGATTTAGTCAAGTTATCGATTCAGGGAAATCTCACCGCCCCACAGTCGTGCAAAATTAATCAggg                                                               <  1:212633/140‑1 (MQ=255)                                                                     ccGAAAACGGATATTGCCGTCATTAAAGGCGCGTGGGTCGATGGAATGGGAAGCCCGTCTACAGGTGGCTTCCATGATTTAGTCAAGTTATCGATTCAGGGAAATCTCACCGCCCCACAGTCGTGCAAAATTAATCAggg                                                               <  1:461257/140‑1 (MQ=255)                                                                         aaaCGGATATTGCCGTCATTAAAGGCGCGTGGGTCGATGGAATGGGAAGCCCGTCTACAGGTGGCTTCCATGATTTAGTCAAGTTATCGATTCAGGGAAATCTCACCGCCCCACAGTCGTGCAAAATTAATCAGGGCGAt                                                           <  1:123868/140‑1 (MQ=255)                                                                          aaCGGATATTGCCGTCATTAAAGGCGCGTGGGTCGATGGAATGGGAAGCCCGTCTACAGGTGGCTTCCATGATTTAGTCAAGTTATCGATTCAGGGAAATCTCACCGCCCCACAGTCGTGCAAAATTAAt                                                                    >  1:198342/1‑130 (MQ=255)                                                                          aaCGGATATTGCCGTCATTAAAGGCGCGTGGGTCGATGGAATGGGAAGCCCGTCTACAGGTGGCTTCCATGATTTAGTCAAGTTATCGATTCAGGGAAATCTCACCGCCCCACAGTCGTGCAAAATTAAt                                                                    <  2:198342/130‑1 (MQ=255)                                                                           aCGGATATTGCCGTCATTAAAGGCGCGTGGGTCGATGGAATGGGAAGCCCGTCTACAGGTGGCTTCCATGATTTAGTCAAGTTATCGATTCAGGGAAATCTCACCGCCCCACAGTCGTGCAAAATTAATCAGGGCGATGt                                                         >  1:176684/1‑140 (MQ=255)                                                                                                  cgcgTGGGTCGATGGAATGGGAAGCCCGTCTACAGGTGGCTTCCATGATTTAGTCAAGTTATCGATTCAGGGAAATCTCACCGCCCCACAGTCGTGCAAAATTAATCAGGGCGATGTTATTAAGGTTAATTTTGGATtca                                  >  2:75543/1‑140 (MQ=255)                                                                                                    cgTGGGTCGATGGAATGGGAAGCCCGTCTACAGGTGGCTTCCATGATTTAGTCAAGTTATCGATTCAGGGAAATCTCACCGCCCCACAGTCGTGCAAAATTAATCAGGGCGATGTTATTAAGGTTAATTTTGGATtcatc                                >  2:195969/1‑140 (MQ=255)                                                                                                    cgTGGGTCGATGGAATGGGAAGCCCGTCTACAGGTGGCTTCCATGATTTAGTCAAGTTATCGATTCAGGGAAATCTCACCGCCCCACAGTCGTGCAAAATTAATCAGGGCGATGTTATTAAGGTTAATTTTGGATtcatc                                >  2:7340/1‑140 (MQ=255)                                                                                                     gTGGGTCGATGGAATGGGAAGCCCGTCTACAGGTGGCTTCCATGATTTAGTCAAGTTATCGATTCAGGGAAATCTCACCGCCCCACAGTCGTGCAAAATTAATCAGGGCGATGTTATTAAGGTTAATTTTGGATtcatca                               <  2:148884/140‑1 (MQ=255)                                                                                                     gTGGGTCGATGGAATGGGAAGCCCGTCTACAGGTGGCTTCCATGATTTAGTCAAGTTATCGATTCAGGGAAATCTCACCGCCCCACAGTCGTGCAAAATTAATCAGGGCGATGTTATTAAGGTTAATTTTGGATtcatca                               <  1:7340/140‑1 (MQ=255)                                                                                                          tCGATGGAATGGGAAGCCCGTCTACAGGTGGCTTCCATGATTTAGTCAAGTTATCGATTCAGGGAAATCTCACCGCCCCACAGTCGTGCAAAATTAATCAGGGCGATGTTATTAAGGTTAATTTTGGATTCATCAATGGt                          <  1:75543/140‑1 (MQ=255)                                                                                                          tCGATGGAATGGGAAGCCCGTCTACAGGTGGCTTCCATGATTTAGTCAAGTTATCGATTCAGGGAAATCTCACCGCCCCACAGTCGTGCAAAATTAATCAGGGCGATGTTATTAAGGTTAATTTTGGATTCATCAATGGt                          <  1:514666/140‑1 (MQ=255)                                                                                                             aTGGAATGGGAAGCCCGTCTACAGGTGGCTTCCATGATTTAGTCAAGTTATCGATTCAGGGAAATCTCACCGCCCCACAGTCGTGCAAAATTAATCAGGGCGATGTTATTAAGGTTAATTTTGGATTCATCAATGGTCAg                       >  1:233072/1‑140 (MQ=255)                                                                                                                          cccGTCTACAGGTGGCTTCCATGATTTAGTCAAGTTATCGATTCAGGGAAATCTCACCGCCCCACAGTCGTGCAAAATTAATCAGGGCGATGTTATTAAGGTTAATTTTGGATTCATCAATGGTCAGAAGTTTACCAccc          >  2:342087/1‑140 (MQ=255)                                                                                                                           ccGTCTACAGGTGGCTTCCATGATTTAGTCAAGTTATCGATTCAGGGAAATCTCACCGCCCCACAGTCGTGCAAAATTAATCAGGGCGATGTTATTAAGGTTAATTTTGGATTCATCAATGGTCAGAAGTTTACCACCCg         >  1:539484/1‑140 (MQ=255)                                                                                                                              tCTACAGGTGGCTTCCATGATTTAGTCAAGTTATCGATTCAGGGAAATCTCACCGCCCCACAGTCGTGCAAAATTAATCAGGGCGATGTTATTAAGGTTAATTTTGGATTCATCAATGGTCAGAAGTTTACCACCCGCaa      >  1:170430/1‑140 (MQ=255)                                                                                                                                  cAGGTGGCTTCCATGATTTAGTCAAGTTATCGATTCAGGGAAATCTCACCGCCCCACAGTCGTGCAAAATTAATCAGGGCGATGTTATTAAGGTTAATTTTGGATTCATCAATGGTCAGAAGTTTACCACCCGCAatgcc  <  2:233072/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      TTAATATTGGTGCGAACACCACCTTTACCCTGTATGTCACCAAGCCATTTCTCGGCTCGATGACCATTCCGAAAACGGATATTGCCGTCATTAAAGGCGCGTGGGTCGATGGAATGGGAAGCCCGTCTACAGGTGACTTCCATGATTTAGTCAAGTTATCGATTCAGGGAAATCTCACCGCCCCACAGTCGTGCAAAATTAATCAGGGCGATGTTATTAAGGTTAATTTTGGATTCATCAATGGTCAGAAGTTTACCACCCGCAATGCC  >  NC_000913/3291813‑3292081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑