Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	239,850	C→T	A114T (GCA→ACA)	yafV ←	2‑oxoglutaramate amidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	239,850	0	C	T	100.0%	84.6 / NA	25	A114T (GCA→ACA)	yafV	2‑oxoglutaramate amidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/9);  total (16/9)

GGTCATAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGCAATCAGCGCATTGCACTGCTGCGCCTT  >  NC_000913/239718‑239985                                                                                                                                     |                                                                                                                                        ggTCATAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGTCATGCgg                                                                                                                                  >  1:136065/1‑140 (MQ=255)            ttGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGTCATGCGGAACAGATGACg                                                                                                                       >  2:188731/1‑140 (MQ=255)                     cgcgACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGTCATGCGGAACAGATGACGCTTATCATa                                                                                                              >  1:447212/1‑140 (MQ=255)                                            gTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGTCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCt                                                                                       >  1:486090/1‑140 (MQ=255)                                            gTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGTCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCt                                                                                       >  1:241709/1‑140 (MQ=255)                                               gTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGTCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCaa                                                                                    <  2:241709/140‑1 (MQ=255)                                                        cAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGTCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCaaaa                                                                           >  2:497070/1‑140 (MQ=255)                                                             gCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGTCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCgg                                                                      >  2:359998/1‑140 (MQ=255)                                                                 aaTACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGTCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTaa                                                                  <  2:143819/140‑1 (MQ=255)                                                                       ccAGCCGCGCCATTCCACAACCACTCGCGCATTGCCCGCTTTATAATGTAGATACTCATCTGTCATGCGGAACAGATGACGCTTATCATAAAAATGTCCCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAAc                                                            <  2:301900/140‑1 (MQ=255)                                                                            cgcgCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGTCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCATCAAAAAGCGGTTAACCGAACCAGAc                                                       <  1:188731/140‑1 (MQ=255)                                                                            cgcgCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGTCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGAc                                                       <  1:174524/140‑1 (MQ=255)                                                                                 cATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGTCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGt                                                  <  1:336079/140‑1 (MQ=255)                                                                                              cTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGTCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAaca                                     >  1:319715/1‑140 (MQ=255)                                                                                               tCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGTCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAacac                                    <  1:497070/140‑1 (MQ=255)                                                                                                  cgcATTGCCCGCTTTATAATGTAGATGCTCATCTGTCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCAGTTAACCGAACCAGACTCCGTTTGTAATGCAACActgc                                 >  1:16471/1‑140 (MQ=255)                                                                                                       tGCCCGCTTTATAATGTAGATTCTCATCTGTCATGCGGAACAGATGCCGCTTATCATAAAAATGTACCGTGCCGCACCGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGCa                            >  2:225419/1‑140 (MQ=255)                                                                                                              tttATAATGTAGATGCTCATCTGTCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGCAATCAgcg                     <  2:486090/140‑1 (MQ=255)                                                                                                                    aTGTAGATGCTCATCTGTCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGCAATCAGCGCATTGc               <  1:359998/140‑1 (MQ=255)                                                                                                                          aTGCTCATCTGTCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCTAACCAGACTCCGTTTGTAATGCAACACTGCCTGCAATCAGCGCATTGCActgct         >  1:45111/1‑140 (MQ=255)                                                                                                                          aTGCTCATCTGTCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGCAATCAGCGCATTGc               >  1:155261/1‑134 (MQ=255)                                                                                                                          aTGCTCATCTGTCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGCAATCAGCGCATTGc               <  2:155261/134‑1 (MQ=255)                                                                                                                          aTGCTCATCTGTCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGCAATCAGCGCATTGCActgct         >  2:12629/1‑140 (MQ=255)                                                                                                                          aTGCTCATCTGTCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGCAATCAGCGCATTGCActgct         >  1:540612/1‑140 (MQ=255)                                                                                                                               cATCTGTCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGCAATCAGCGCATTGCACTGCTGCGcc    >  2:190472/1‑140 (MQ=255)                                                                                                                                 tCTGTCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGACTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGCAATCAGCGCATTGCACTGCTGCGCCtt  >  2:277883/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                        GGTCATAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGCAATCAGCGCATTGCACTGCTGCGCCTT  >  NC_000913/239718‑239985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑