Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,374,862	T→C	intergenic (‑373/‑7)	nanR ← / → dcuD	DNA‑binding transcriptional dual regulator NanR/putative transporter DcuD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,374,862	0	T	C	100.0%	137.5 / NA	38	intergenic (‑373/‑7)	nanR/dcuD	DNA‑binding transcriptional dual regulator NanR/putative transporter DcuD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/17);  total (21/17)

CATATTTTTTAATGAAAAATCACCTCACCTACAATTAAAAACACGACATCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGT  >  NC_000913/3374725‑3374992                                                                                                                                          |                                                                                                                                   cATATTTTTTAATGAAAAATCACCTCACCTACAATTAAAAACACGACATCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTc                                                                                                                                  >  2:164515/1‑140 (MQ=255) cATATTTTTTAATGAAAAATCACCTCACCTACAATTAAAAACACGACATCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTc                                                                                                                                  >  1:502984/1‑140 (MQ=255)          tAATGAAAAATCACCTCACCTACAATTAAAAACACGACATCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTCAACAATGtt                                                                                                                         <  2:70821/140‑1 (MQ=255)            aTGAAAAATCACCTCACCTACAATTAAAAACACGACATCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCg                                                                                                                       >  1:85234/1‑140 (MQ=255)             tGAAAAATCACCTCACCTACAATTAAAAACACGACATCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCgg                                                                                                                      <  2:85234/140‑1 (MQ=255)              gAAAAATCACCTCACCTACAATTAAAAACACGACATCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGc                                                                                                                     >  1:307868/1‑140 (MQ=255)                            cctACAATTAAAAACACGACATCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGt                                                                                                       >  2:451637/1‑140 (MQ=255)                             ctACAATTAAAAACACGACATCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTc                                                                                                      <  1:164515/140‑1 (MQ=255)                                       aaaCACGACATCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTattaa                                                                                            <  2:307371/140‑1 (MQ=255)                                             gACATCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGa                                                                                      >  2:487488/1‑140 (MQ=255)                                             gACATCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGa                                                                                      <  1:192448/140‑1 (MQ=255)                                             gACATCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGa                                                                                      >  1:86404/1‑140 (MQ=255)                                               cATCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATg                                                                                    <  2:307868/140‑1 (MQ=255)                                               cATCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATg                                                                                    <  2:86404/140‑1 (MQ=255)                                                         aTAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGa                                                                          >  1:482721/1‑140 (MQ=255)                                                          tAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGAt                                                                         >  2:67852/1‑140 (MQ=255)                                                                 ccTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGaaa                                                                  >  2:341515/1‑140 (MQ=255)                                                                      cAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTa                                                             >  2:205889/1‑140 (MQ=255)                                                                           aaTATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAAc                                                        >  1:31024/1‑140 (MQ=255)                                                                                 aCATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAgg                                                  <  2:143991/140‑1 (MQ=255)                                                                                     cGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAggtggt                                              >  2:102051/1‑140 (MQ=255)                                                                                     cGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAggtggt                                              >  2:51590/1‑140 (MQ=255)                                                                                             tCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTgctggctg                                      <  1:51590/140‑1 (MQ=255)                                                                                             tCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTgctggctg                                      <  1:166578/140‑1 (MQ=255)                                                                                             tCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTgctggctg                                      <  2:31024/140‑1 (MQ=255)                                                                                                   tGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAg                                <  1:155742/140‑1 (MQ=255)                                                                                                    gCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAgg                               >  2:369171/1‑140 (MQ=255)                                                                                                    gCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAgg                               >  2:137271/1‑140 (MQ=255)                                                                                                     ccGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGt                              >  2:52786/1‑140 (MQ=255)                                                                                                       gTTTATGGGATTGACGGTTTTCTTTTGCCACGGAGCTTACCACTGTTCGTCATAAGTATATCTATCATCGTATTAATTACGATGGGATATTTGTTCCTGAAAAACTACAAACCTCAGGTTGTGCTGGCTGCCGCAGGTAt                            <  2:502984/140‑1 (MQ=255)                                                                                                       gTTTATGGGATTGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTAt                            <  1:205889/140‑1 (MQ=255)                                                                                                                  tGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTc                        >  2:294320/1‑133 (MQ=255)                                                                                                                  tGACCGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTc                        <  1:294320/133‑1 (MQ=255)                                                                                                                    aCCGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGatgatg               >  1:417609/1‑140 (MQ=255)                                                                                                                      cGTTTTCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGAtgtg             <  2:482721/140‑1 (MQ=255)                                                                                                                        ttttCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCg           <  1:341515/140‑1 (MQ=255)                                                                                                                         tttCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCgg          >  2:321194/1‑140 (MQ=255)                                                                                                                         tttCTTTTGACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCgg          >  2:401291/1‑140 (MQ=255)                                                                                                                                 gACACGGAGCTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGt  <  1:321194/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                   CATATTTTTTAATGAAAAATCACCTCACCTACAATTAAAAACACGACATCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGT  >  NC_000913/3374725‑3374992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑