Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,388,726	A→G	V134A (GTT→GCT)	aaeA ←	aromatic carboxylic acid efflux pump membrane fusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,388,726	0	A	G	100.0%	114.8 / NA	32	V134A (GTT→GCT)	aaeA	aromatic carboxylic acid efflux pump membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/17);  total (15/17)

CCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGATAATAAGCAACATCGGCTTGCGC  >  NC_000913/3388591‑3388859                                                                                                                                        |                                                                                                                                      ccTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGt                                                                                                                                   <  2:137962/140‑1 (MQ=255)    cGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTg                                                                                                                                >  2:493005/1‑140 (MQ=255)         aataaACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTgtagta                                                                                                                           <  2:375094/140‑1 (MQ=255)                  aCCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTgg                                                                                                                  >  2:30600/1‑140 (MQ=255)                  aCCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTgg                                                                                                                  >  2:76264/1‑140 (MQ=255)                      gTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTg                                                                                                              >  1:206660/1‑140 (MQ=255)                        ataGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGt                                                                                                            <  2:206660/140‑1 (MQ=255)                            aCGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGAt                                                                                                        <  2:468394/140‑1 (MQ=255)                              gTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGAtct                                                                                                      >  2:301729/1‑140 (MQ=255)                                tGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATctct                                                                                                    >  1:373703/1‑140 (MQ=255)                                 gAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCtt                                                                                                   >  1:66029/1‑140 (MQ=255)                                    gTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTcgc                                                                                                <  1:301572/140‑1 (MQ=255)                                                                cgGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCgaga                                                                         <  1:19795/135‑1 (MQ=255)                                                                cgGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCgaga                                                                         >  2:19795/1‑135 (MQ=255)                                                                cgGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGtt                                                                    >  2:63494/1‑140 (MQ=255)                                                                  gATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCACTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTac                                                                  >  1:369741/1‑140 (MQ=255)                                                                          tGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCg                                                          <  1:493005/140‑1 (MQ=255)                                                                             gTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTacgac                                                               <  1:61411/132‑1 (MQ=255)                                                                             gTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTacgac                                                               >  2:61411/1‑132 (MQ=255)                                                                                 aaGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGcctcct                                                   <  1:301729/140‑1 (MQ=255)                                                                                      ccAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGt                                              <  1:30600/140‑1 (MQ=255)                                                                                            ttGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTcc                                        <  2:183705/140‑1 (MQ=255)                                                                                            ttGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTcc                                        >  1:389455/1‑140 (MQ=255)                                                                                             tGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCt                                       <  1:277302/140‑1 (MQ=255)                                                                                                 aGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGc                                   <  2:389455/140‑1 (MQ=255)                                                                                                   aTCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCa                                 <  1:15054/140‑1 (MQ=255)                                                                                                       cgGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTAc                             <  2:201440/140‑1 (MQ=255)                                                                                                        gggTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCATTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTAcc                            <  2:373703/140‑1 (MQ=255)                                                                                                                  cgcTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGATAATAAg                  <  1:44007/140‑1 (MQ=255)                                                                                                                                 aTGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGATAATAAGCAACATCGGCTTgcg   >  2:321982/1‑140 (MQ=255)                                                                                                                                 aTGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGATAATAAGCAACATCGGCTTgcg   >  2:436069/1‑140 (MQ=255)                                                                                                                                  tGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGATAATAAGCAACATCGGCTTgcgc  >  1:133700/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      CCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGATAATAAGCAACATCGGCTTGCGC  >  NC_000913/3388591‑3388859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑