Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,430,461	G→A	G126G (GGC→GGT)	aroE ←	shikimate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,430,461	0	G	A	100.0%	91.6 / NA	28	G126G (GGC→GGT)	aroE	shikimate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/14);  total (14/14)

CAACGCCTGAATACTGCCAGTGTGCGCAAACAATTTAGCCAACTCTTCCGCGCGGGATACCGTCCGATTAGTTATTGTCACCGCACAGTCCAGGGAAAGGAGTGGCAGTAGTACGCCGCGAGATGCTCCACCAGCGCCGATAAGCAGAATACGTAAACCAGGGCGGATAAAAGACAGACGTTCCAGATCGCTTAACAAGCCTACACCATCGGTATTGTCACCCAGCAGGCGTCCATCTTCTAACCGCATGAGGGTATTAACAGCACCAGCC  >  NC_000913/3430326‑3430596                                                                                                                                        |                                                                                                                                        cAACGCCTGAATACTGCCAGTGTGCGCAAACAATTTAGCCAACTCTTCCGCGCGGGATACCGTCCGATTAGTTATTGTCACCGCACAGTCCAGGGAAAGGAGTGGCAGTAGTACGCCGCGAGATGCTCCACCAGCACCGa                                                                                                                                     <  1:121202/140‑1 (MQ=255)  aaCGCCTGAATACTGCCAGTGTGCGCAAACAATTTAGCCAACTCTTCCGCGCGGGATACCGTCCGATTAGTTATTGTCACCGCACAGTCCAGGGAAAGGAGTGGCAGTAGTACGCCGCGAGATGCTCCACCAGCACCGAt                                                                                                                                    >  1:315217/1‑140 (MQ=255)     gCCTGAATACTGCCAGTGTGCGCAAACAATTTAGCCAACTCTTCCGCGCGGGATACCGTCCGATTAGTTATTGTCACCGCACAGTCCAGGGAAAGGAGTGGCAGTAGTACGCCGCGAGATGCTCCACCAGCACCGATAAg                                                                                                                                 <  2:315217/140‑1 (MQ=255)         gAATACTGCCAGTGTGCGCAAACAATTTAGCCAACTCTTCCGCGCGGGATACCGTCCGATTAGTTATTGTCACCGCACAGTCCAGGGAAAGGAGTGGCAGTAGTACGCCGCGAGATGCTCCACCAGCACCGATAAGCAGa                                                                                                                             <  2:292554/140‑1 (MQ=255)         gAATACTGCCAGTGTGCGCAAACAATTTAGCCAACTCTTCCGCGCGGGATACCGTCCGATTAGTTATTGTCACCGCACAGTCCAGGGAAAGGAGTGGCAGTAGTACGCCGCGAGATGCTCCACCAGCACCGATAAGCAGa                                                                                                                             <  2:194181/140‑1 (MQ=255)                 ccAGTGTGCGCAAACAATTTAGCCAACTCTTCCGCGCGGGATACCGTCCGATTAGTTATTGTCACCGCACAGTCCAGGGAAAGGAGTGGCAGTAGTACGCCGCGAGATGCTCCACCAGCACCGATAAGCAGAATACGTaa                                                                                                                     >  2:91304/1‑140 (MQ=255)                    gtgtGCGCAAACAATTTAGCCAACTCTTCCGCGCGGGATACCGTCCGATTAGTTATTGTCACCGCACAGTCCAGGGAAAGGAGTGGCAGTAGTACGCCGCGAGATGCTCCACCAGCACCGATAAGCAGAATACGTAAAcc                                                                                                                  >  1:460103/1‑140 (MQ=255)                                              ttCCGCGCGGGATACCGTCCGATTAGTTATTGTCACCGCACAGTCCAGGGAAAGGAGTGGCAGTAGTACGCCGCGAGATGCTCCACCAGCACCGATAAGCAGAATACGTAAACCAGGGCGGATAAAAGACAGACGTTCCa                                                                                        <  1:162935/140‑1 (MQ=255)                                                              ttCCGATTAGTTATTGTCACCGCACAGTCCAGGGAAAGGAGTGGCAGTAATACGCCGCGAGATGCTCCACCAGCACCGATAAGCAGAATACGTAAACCAGGGCGGATAAAAGACAGACGTTCCAGATCGCTTAACAAGcc                                                                        <  1:246974/139‑1 (MQ=255)                                                               tCCGATTAGTTATTGTCACCGCACAGTCCAGGGAAAGGAGTGGCAGTAGTACGCCGCGAGATGCTCCACCAGCACCGATAAGCAGAATACGTAAACCAGGGCGGATAAAAGACAGACGTTCCAGATCGCTTAACAAGCCt                                                                       >  1:525244/1‑140 (MQ=255)                                                                ccGATTAGTTATTGTCACCGCACAGTCCAGGGAAAGGAGTGGCAGTAGTACGCCGCGAGATGCTCCACCAGCACCGATAAGTAGAATACGTAAACCAGGGCGGATAAAAGACAGACGTTCGAGATCGCTTAACAAGCCTa                                                                      <  2:219627/140‑1 (MQ=255)                                                                ccGATTAGTTATTGTCACCGCACAGTCCAGGGAAAGGAGTGGCAGTAGTACGCCGCGAGATGCTCCACCAGCACCGATAAGCAGAATACGTAAACCAGGGCGGATAAAAGACAGACGTTCCAGATCGCTTAACAAGCCTa                                                                      >  1:150260/1‑140 (MQ=255)                                                                ccGATTAGTTATTGTCACCGCACAGTCCAGGGAAAGGAGTGGCAGTAGTACGCCGCGAGATGCTCCACCAGCACCGATAAGCAGAATACGTAAACCAGGGCGGATAAAAGACAGACGTTCCAGATCGCTTAACAAGCCTa                                                                      >  1:303083/1‑140 (MQ=255)                                                                  gATTAGTTATTGTCACCGCACAGTCCAGGGAAAGGAGTGGCAGTAGTACGCCGCGAGATGCTCCACCAGCACCGATAAGCAGAATACGTAAACCAGGGCGGATAAAAGACAGACGTTCCAGATCGCTTAACAAGCCTaca                                                                    >  2:458043/1‑140 (MQ=255)                                                                  gATTAGTTATTGTCACCGCACAGTCCAGGGAAAGGAGTGGCAGTAGTACGCCGCGAGATGCTCCACCAGCACCGATAAGCAGAATACGTAAACCAGGGCGGATAAAAGACAGACGTTCCAGATCGCTTAACAAGCCTaca                                                                    >  1:344426/1‑140 (MQ=255)                                                                       gTTATTGTCACCGCACAGTCCAGGGAAAGGAGTGGCAGTAGTACGCCGCGAGATGCTCCACCAGCACCGATAAGCAGAATACGTAAACCAGGGCGGATAAAAGACAGACGTTCCAGATCGCTTAACAAGCCTACACCATc                                                               <  2:96182/140‑1 (MQ=255)                                                                                        gTCCAGGGAAAGGAGTGGCAGTAGTACGCCGCGAGATGCTCCACCAGCACCGATAAGCAGAATACGTAAACCAGGGCGGATAAAAGACAGACGTTCCAGATCGCTTAACAAGCCTACACCATc                                                               >  2:25946/1‑123 (MQ=255)                                                                                        gTCCAGGGAAAGGAGTGGCAGTAGTACGCCGCGAGATGCTCCACCAGCACCGATAAGCAGAATACGTAAACCAGGGCGGATAAAAGACAGACGTTCCAGATCGCTTAACAAGCCTACACCATc                                                               <  1:25946/123‑1 (MQ=255)                                                                                                   ggAGTGGCAGTAGTACGCCGCGAGATGCTCCACCAGCACCGATAAGCAGAATACGTAAACCAGGGCGGATAAAAGACAGACGTTCCAGATCGCTTAACAAGCCTACACCATCGGTATTGTCACCCAGCAGGCGTCCAtct                                   >  1:84852/1‑140 (MQ=255)                                                                                                   ggAGTGGCAGTAGTACGCCGCGAGATGCTCCACCAGCACCGATAAGCAGAATACGTAAACCAGGGCGGATAAAAGACAGACGTTCCAGATCGCTTAACAAGCCTACACCATCGGTATTGTCACCCAGCAGGCGTCCAtct                                   >  2:460493/1‑140 (MQ=255)                                                                                                   ggAGTGGCAGTAGTACGCCGCGAGATGCTCCACCAGCACCGATAAGCAGAATACGTAAACCAGGGCGGATAAAAGACAGACGTTCCAGATCGCTTAACAAGCCTACACCATCGGTATTGTCACCCAGCAGGCGTCCAtct                                   >  1:173125/1‑140 (MQ=255)                                                                                                         gCAGTAGTACGCCGCGAGATGCTCCACCAGCACCGATAAGCAGAATACGTAAACCAGGGCGGATAAAAGACAGACGTTCCAGATCGCTTAACAAGCCTACACCATCGGTATTGTCACCCAGCAGGCGTCCATCTTCTAAc                             <  1:460493/140‑1 (MQ=255)                                                                                                           agtagtACGCCGCGAGATGCTCCACCAGCACCGATAAGCAGAATACGTAAACCAGGGCGGATAAAAGACAGACGTTCCAGATCGCTTAACAAGCCTACACCATCGGTATTGTCACCCAGCAGGCGTCCATCTTCTAACCg                           <  2:303083/140‑1 (MQ=255)                                                                                                           agtagtACGCCGCGAGATGCTCCACCAGCACCGATAAGCAGAATACGTAAACCAGGGCGGATAAAAGACAGACGTTCCAGATCGCTTAACAAGCCTACACCATCGGTATTGTCACCCAGCAGGCGTCCATCTTCTAACCg                           <  2:344426/140‑1 (MQ=255)                                                                                                               gtaCGCCGCGAGATGCTCCACCAGCACCGATAAGCAGAATACGTAAACCAGGGCGGATAAAAGACAGACGTTCCAGATCGCTTAACAAGCCTACACCATCGGTATTGTCACCCAGCAGGCGTCCATCTTCTAACCGCATg                       <  2:525244/140‑1 (MQ=255)                                                                                                               gtaCGCCGCGAGATGCTCCACCAGCACCGATAAGCAGAATACGTAAACCAGGGCGGATAAAAGACAGACGTTCCAGATCGCTTAACAAGCCTACACCATCGGTATTGTCACCCAGCAGGCGTCCATCTTCTAACCGCATg                       <  2:84852/140‑1 (MQ=255)                                                                                                                                 caccaGCACCGATAAGCAGAATACGTAAACCAGGGCGGATAAAAGACAGACGTTCCAGATCGCTTAACAAGCCTACACCATCGGTATTGTCACCCAGCAGGCGTCCATCTTCTAACCGCATGAGGGTATTAACAGCACCa     >  2:409878/1‑140 (MQ=255)                                                                                                                                    caGCACCGATAAGCAGAATACGTAAACCAGGGCGGATAAAAGACAGACGTTCCAGATCGCTTAACAAGCCTACACCATCGGTATTGTCACCCAGCAGGCGTCCATCTTCTAACCGCATGAGGGTATTAACAGCACCAGcc  >  1:144842/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        CAACGCCTGAATACTGCCAGTGTGCGCAAACAATTTAGCCAACTCTTCCGCGCGGGATACCGTCCGATTAGTTATTGTCACCGCACAGTCCAGGGAAAGGAGTGGCAGTAGTACGCCGCGAGATGCTCCACCAGCGCCGATAAGCAGAATACGTAAACCAGGGCGGATAAAAGACAGACGTTCCAGATCGCTTAACAAGCCTACACCATCGGTATTGTCACCCAGCAGGCGTCCATCTTCTAACCGCATGAGGGTATTAACAGCACCAGCC  >  NC_000913/3430326‑3430596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑