| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,489,822 | C→T | R120H (CGC→CAC) | argD ← | N‑acetylornithine aminotransferase/N‑succinyldiaminopimelate aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,489,822 | 0 | C | T | 95.0% | 57.3 / ‑3.6 | 20 | R120H (CGC→CAC) | argD | N‑acetylornithine aminotransferase/N‑succinyldiaminopimelate aminotransferase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (9/10); total (10/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.60e-01 | |||||||||||
GGTTTCGGCCCAAAGCCGTCGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTG > NC_000913/3489687‑3489950 | ggTTTCGGCCCAAAGCCGTCGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGTGTGc < 2:115888/140‑1 (MQ=255) gCCCAAAGCCGTCGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGTGTGCCAGTTTa < 1:315620/140‑1 (MQ=255) gAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGTGTGCCAGTTTAAAGGCGGTTTCGtt < 1:34073/140‑1 (MQ=255) cccacccaCCGAAACGGTAAACAGCGAGCGACCATG‑AAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGTGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGa > 1:118993/1‑140 (MQ=255) cacccaccGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGTGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAg < 2:26529/140‑1 (MQ=255) agcgaCCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGTGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATa < 1:385436/122‑1 (MQ=255) agcgaCCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGTGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATa > 2:385436/1‑122 (MQ=255) gcgaCCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGTGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCaa > 2:495665/1‑140 (MQ=255) tGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGTGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGc < 2:77676/140‑1 (MQ=255) cGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGTGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAG‑TCATAAACACCACGCGTTCGGCAAACGTTGCCTCAAt < 1:355449/138‑1 (MQ=255) cGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGTGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAG‑TCATAAACACCACGCGTTCGGCAAACGTTGCCTCAAt > 2:355449/1‑138 (MQ=255) cacacaGGCGTAATGGTGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGt < 2:509635/140‑1 (MQ=255) acacaGGCGTAATGGTGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGa > 2:385128/1‑133 (MQ=255) acacaGGCGTAATGGTGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGa < 1:385128/133‑1 (MQ=255) acacaGGCGTAATGGTGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGtt > 1:394444/1‑140 (MQ=255) acacaGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGtt > 2:250734/1‑140 (MQ=255) acaGGCGTAATGGTGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTAc > 1:450517/1‑140 (MQ=255) aGGCGTAATGGTGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTg > 1:502422/1‑140 (MQ=255) aGGCGTAATGGTGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTg < 1:495665/140‑1 (MQ=255) aGGCGTAATGGTGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCAAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTg > 2:322911/1‑140 (MQ=255) | GGTTTCGGCCCAAAGCCGTCGGAATATTTTGGCTGCCCACCCACCGAAACGGTAAACAGCGAGCGACCATGAAAAGCGTTATGGAAGGCAATAATTTTGGTTTTGAACGGGCTATGACGCACACAGGCGTAATGGCGTGCCAGTTTAAAGGCGGTTTCGTTAGCTTCCGTGCCGGAGTTCATAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTG > NC_000913/3489687‑3489950 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |