Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,502,200	G→A	V311V (GTG→GTA)	frlB →	fructoselysine 6‑phosphate deglycase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,502,200	0	G	A	100.0%	133.1 / NA	39	V311V (GTG→GTA)	frlB	fructoselysine 6‑phosphate deglycase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/21);  total (18/21)

CAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGT  >  NC_000913/3502065‑3502333                                                                                                                                        |                                                                                                                                      cAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCaa                                                                                                                                   <  2:7651/140‑1 (MQ=255) cAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCaa                                                                                                                                   <  1:299269/140‑1 (MQ=255)      atgaAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAg                                                                                                                              <  1:412080/140‑1 (MQ=255)          aaGTCGCCACACCACCGACCGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCTCCCGTGGCTGGCCCCGTTCCTGATGTTCGTACCAATGGAGTGGc                                                                                                                          <  2:376748/140‑1 (MQ=255)           aGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGct                                                                                                                         <  1:460863/140‑1 (MQ=255)           aGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGct                                                                                                                         <  1:6684/140‑1 (MQ=255)           aGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGct                                                                                                                         <  1:279473/140‑1 (MQ=255)              cGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTg                                                                                                                      >  1:416612/1‑140 (MQ=255)              cGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTg                                                                                                                      >  1:165585/1‑140 (MQ=255)              cGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTg                                                                                                                      >  2:409194/1‑140 (MQ=255)                ccACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACTAATGGAGTGGCTCTGct                                                                                                                    >  1:168070/1‑140 (MQ=255)                ccACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGct                                                                                                                    >  1:27898/1‑140 (MQ=255)                        ccGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTg                                                                                                            >  2:155170/1‑140 (MQ=255)                         cGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCAGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGt                                                                                                           <  1:78693/140‑1 (MQ=255)                                  cATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACa                                                                                                  <  2:193084/140‑1 (MQ=255)                                   aTTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCACAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACaa                                                                                                 >  2:365333/1‑140 (MQ=255)                                       aCTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGAt                                                                                             <  1:409194/140‑1 (MQ=255)                                            gTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACaa                                                                                        >  1:419830/1‑140 (MQ=255)                                                     cGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACaa                                                                                                 <  2:463201/122‑1 (MQ=255)                                                     cGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACaa                                                                                                 >  1:463201/1‑122 (MQ=255)                                                     cGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGa                                                                               >  1:4640/1‑140 (MQ=255)                                                      gTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGaa                                                                              <  2:27898/140‑1 (MQ=255)                                                         cTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAAcgc                                                                           <  1:155170/140‑1 (MQ=255)                                                            aCAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAAcgccgc                                                                        <  2:60932/140‑1 (MQ=255)                                                              aaCGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTa                                                                      <  1:10551/140‑1 (MQ=255)                                                              aaCGTGATCATCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAAcgccg                                                                         >  1:73816/1‑137 (MQ=255)                                                              aaCGTGATCATCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAAcgccg                                                                         <  2:73816/137‑1 (MQ=255)                                                                 gTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTa                                                                   <  2:165585/140‑1 (MQ=255)                                                                          aTCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCt                                                          >  1:479123/1‑140 (MQ=255)                                                                                tACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGa                                                    <  2:4640/140‑1 (MQ=255)                                                                                  cGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAat                                                  >  2:288/1‑140 (MQ=255)                                                                                   gCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTc                                                           >  2:110978/1‑130 (MQ=255)                                                                                   gCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTc                                                           <  1:110978/130‑1 (MQ=255)                                                                                   gCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAata                                                 >  1:512897/1‑140 (MQ=255)                                                                                                    gCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTcccggc                                <  2:168676/140‑1 (MQ=255)                                                                                                                      aCCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTCCTTCCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCCGGCAACGGGCCGGAtt              <  2:416612/140‑1 (MQ=255)                                                                                                                      aCCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCCGGCAACGGGCCGGAtt              <  1:365333/140‑1 (MQ=255)                                                                                                                          ttCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCCGGCAACGGGCCGGATTTACg          >  2:427077/1‑140 (MQ=255)                                                                                                                          ttCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCCGGCAACGGGCCGGATTTACg          >  2:493311/1‑140 (MQ=255)                                                                                                                            ccTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCCGGCAACGGGCCGGATTTACGCa        <  1:48488/140‑1 (MQ=255)                                                                                                                                  gTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGt  >  2:384340/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      CAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGT  >  NC_000913/3502065‑3502333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑