Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,516,831	G→A	P159L (CCG→CTG)	damX ←	cell division protein DamX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,516,831	0	G	A	100.0%	85.0 / NA	25	P159L (CCG→CTG)	damX	cell division protein DamX
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/8);  total (17/8)

TTGTTCAGGTCACCCTGCACTTCAACACGTTGTTGACCATCCGTTGCCACCGGGGTTTGCCCTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATC  >  NC_000913/3516699‑3516959                                                                                                                                     |                                                                                                                                 ttGTTCAGGTCACCCTGCACTTCAACACGTTGTTGACCATCCGTTGCCACCGGGGTTTGCCCTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCgctgg                                                                                                                           >  1:423571/1‑140 (MQ=255)              ccTGCACTTCAACACGTTGTTGACCATCCGTTGCCACCGGGGTTTGCCCTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTc                                                                                                              >  1:357053/1‑140 (MQ=255)                   aCTTCAACACGTTGTTGACCATCCGTTGCCACCGGGGTTTGCCCTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCa                                                                                                                 >  2:85500/1‑132 (MQ=255)                   aCTTCAACACGTTGTTGACCATCCGTTGCCACCGGGGTTTGCCCTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCa                                                                                                                 <  1:85500/132‑1 (MQ=255)                   aCTTCAACACGTTGTTGACCATCCGTTGCCACCGGGGTTTGCCCTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTg                                                                                                         >  2:202701/1‑140 (MQ=255)                   aCTTCAACACGTTGTTGACCATCCGTTGCCACCGGGGTTTGCCCTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTg                                                                                                         >  1:515305/1‑140 (MQ=255)                          cacGTTGTTGACCATCCGTTGCCACCGGGGTTTGCCCTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTAGTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTc                                                                                                  >  2:470804/1‑140 (MQ=255)                                  tgACCATCCGTTGCCACCGGGGTTTGCCCTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGcc                                                                                          <  2:357053/140‑1 (MQ=255)                                       aTCCGTTGCCACCGGGGTTTGCCCTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCg                                                                                                 <  1:260334/128‑1 (MQ=255)                                       aTCCGTTGCCACCGGGGTTTGCCCTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCg                                                                                                 >  2:260334/1‑128 (MQ=255)                                         ccGTTGCCACCGGGGTTTGCCCTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGa                                                                                   >  2:32213/1‑140 (MQ=255)                                                    ggggTTTGCCCTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACtctt                                                                        >  2:184437/1‑140 (MQ=255)                                                             ccTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGa                                                               <  1:470804/140‑1 (MQ=255)                                                                      ggCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTg                                                      <  1:427767/140‑1 (MQ=255)                                                                          tAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCg                                                  <  2:423571/140‑1 (MQ=255)                                                                                  aTCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGt                                          >  1:138154/1‑140 (MQ=255)                                                                                    cGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTgg                                        >  2:367507/1‑140 (MQ=255)                                                                                    cGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTgg                                        >  1:296830/1‑140 (MQ=255)                                                                                         gCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTTGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAg                                   <  1:126272/140‑1 (MQ=255)                                                                                                    tCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAg                        >  2:277010/1‑140 (MQ=255)                                                                                                    tCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAg                        >  2:218597/1‑140 (MQ=255)                                                                                                       tgctgAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAgcgc                     >  1:488199/1‑140 (MQ=255)                                                                                                          tgAGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGa                  <  2:296830/140‑1 (MQ=255)                                                                                                            aGTATTTTCCGCAGACGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAac                >  2:478378/1‑140 (MQ=255)                                                                                                                      gCAGGCGAGGCTCCCATCGCTGGGTGCACGCCATTCGACTGATTGGACGCATAGCCAGCAAGATCAATACTCTTATCGGCGGACGCGGTTTGAACGCTGGAAGTGGTCGCTGGGGCTTTTAGCGCAGAaccgataccgat      >  2:421205/1‑140 (MQ=255)                                                                                                                          aCGTGGTTCCCAGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATc  >  1:301514/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                 TTGTTCAGGTCACCCTGCACTTCAACACGTTGTTGACCATCCGTTGCCACCGGGGTTTGCCCTTGAGTCGGCGTAGAAGAGATCGGTGGCAGAGAAACATCCTGCTGAGTATTTTCCGCAGACGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATC  >  NC_000913/3516699‑3516959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑