Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,541,499 T→C S365P (TCC→CCC)  feoB → Fe(2(+)) transporter FeoB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,541,4990TC100.0% 151.8 / NA 42S365P (TCC→CCC) feoBFe(2(+)) transporter FeoB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/19);  total (23/19)

GCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGACTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGTTTCGGTTGTAACGTACCGTCGGTAATGG  >  NC_000913/3541363‑3541634
                                                                                                                                        |                                                                                                                                       
gCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGACTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCccct                                                                                                                                      >  1:327425/1‑140 (MQ=255)
     gTATTCAATGGATTGGCTACACGCTCCACTTCCCGGACTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCccttcctt                                                                                                                                 >  2:54847/1‑140 (MQ=255)
         tCAATGGATTGGCTACACGCTCCACTTCCCGGACTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAgg                                                                                                                             <  1:11858/140‑1 (MQ=255)
         tCAATGGATTGGCTACACGCTCCACTTCCCGGACTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAgg                                                                                                                             <  1:54847/140‑1 (MQ=255)
           aaTGGATTGGCTACACGCTCCACTTCCCGGACTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGAc                                                                                                                           >  1:444113/1‑140 (MQ=255)
             tGGATTGGCTACACGCTCCACTTCCCGGACTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTc                                                                                                                         <  1:319594/140‑1 (MQ=255)
              ggATTGGCTACACGCTCCACTTCCCGGACTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTg                                                                                                                                >  2:295106/1‑132 (MQ=255)
              ggATTGGCTACACGCTCCACTTCCCGGACTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTg                                                                                                                                <  1:295106/132‑1 (MQ=255)
                aTTGGCTACACGCTCCACTTCCCGGACTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCgg                                                                                                                      >  1:509331/1‑140 (MQ=255)
                aTTGGCTACACGCTCCACTTCCCGGACTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCgg                                                                                                                      >  1:365159/1‑140 (MQ=255)
                         acGCTCCACTTCCCGGACTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATGgc                                                                                                             >  1:323170/1‑140 (MQ=255)
                              ccACTTCCCGGACTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTg                                                                                                        >  1:433051/1‑140 (MQ=255)
                                aCTTCCCGGACTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTgcg                                                                                                      <  1:277847/140‑1 (MQ=255)
                                aCTTCCCGGACTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTgcg                                                                                                      <  1:483044/140‑1 (MQ=255)
                                    cccGGACTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTTCCTGTTCCTCCCCTTCCTTGAGGACTCCGCGTCTATGGCGCGTGCGGCGt                                                                                                  <  2:210588/140‑1 (MQ=255)
                                    cccGGACTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGt                                                                                                  <  2:433051/140‑1 (MQ=255)
                                         aCTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATTGCGCGTGCGGCGTTtgtg                                                                                             >  2:466106/1‑140 (MQ=255)
                                            ggCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATg                                                                                          >  1:44188/1‑140 (MQ=255)
                                              cTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGa                                                                                        <  2:510931/140‑1 (MQ=255)
                                                 aCTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCg                                                                                     >  2:490917/1‑140 (MQ=255)
                                                         ccTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGc                                                                             <  2:323170/140‑1 (MQ=255)
                                                            cGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAgg                                                                          >  1:30735/1‑140 (MQ=255)
                                                            cGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAgg                                                                          >  1:446583/1‑140 (MQ=255)
                                                                   ggCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGc                                                                             <  1:198827/130‑1 (MQ=255)
                                                                   ggCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGc                                                                             >  2:198827/1‑130 (MQ=255)
                                                                        gggTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGc                                                              <  1:182305/140‑1 (MQ=255)
                                                                              cggcATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGAGGa                                                        >  2:278766/1‑140 (MQ=255)
                                                                               ggcATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATTGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGaa                                                       <  1:466106/140‑1 (MQ=255)
                                                                                 cATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAAt                                                     >  1:7738/1‑140 (MQ=255)
                                                                                           gTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGcc                                           >  2:97203/1‑140 (MQ=255)
                                                                                            tgctgcCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCg                                          >  2:428648/1‑140 (MQ=255)
                                                                                            tgctgcCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCg                                          >  1:162/1‑140 (MQ=255)
                                                                                                         gCCGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGtcggtt                             <  2:277750/140‑1 (MQ=255)
                                                                                                          ccGCAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGtcggttt                            <  2:365159/140‑1 (MQ=255)
                                                                                                             cAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGtcggtttcgg                         <  2:30735/140‑1 (MQ=255)
                                                                                                             cAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGtcggtttcgg                         >  2:308448/1‑140 (MQ=255)
                                                                                                             cAGATTGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGtcggtttcgg                         >  2:525790/1‑140 (MQ=255)
                                                                                                                 ttGGCATGATGTACCTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGTTTCGGTTGt                     <  2:74928/140‑1 (MQ=255)
                                                                                                                       tgatgTACCTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGTTTCGGTTGTAACGTa               <  2:446583/140‑1 (MQ=255)
                                                                                                                              ccTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGTTTCGGTTGTAACGTACCGTCgg        >  1:300938/1‑140 (MQ=255)
                                                                                                                              ccTGTTCCTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCACTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGTTTCGGTTGTAACGTACCGTCgg        >  1:116959/1‑140 (MQ=255)
                                                                                                                                  ttCCTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGTTTCGGTTGTAACGTACCGTCGGTAAt    <  1:255141/140‑1 (MQ=255)
                                                                                                                                   tcctccCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGTTTCGGTTGTAACGTACCGTCGGTAATg   >  2:22010/1‑140 (MQ=255)
                                                                                                                                    ccTCCCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGTTTCGGTTGTAACGTACCGTCGGTAATgg  >  1:138560/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                       
GCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGACTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGTTTCGGTTGTAACGTACCGTCGGTAATGG  >  NC_000913/3541363‑3541634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑