Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	247,481	C→T	intergenic (+20/‑156)	yafL → / → rayT	NlpC/P60 family protein YafL/REP‑associated tyrosine transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	247,481	0	C	T	100.0%	86.7 / NA	26	intergenic (+20/‑156)	yafL/rayT	NlpC/P60 family protein YafL/REP‑associated tyrosine transposase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/18);  total (8/18)

GAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAAC  >  NC_000913/247351‑247597                                                                                                                                   |                                                                                                                     gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGTCGCATCCgg                                                                                                             <  2:253191/140‑1 (MQ=255)    tCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGTCGCATCCGGCAg                                                                                                          <  2:26856/140‑1 (MQ=255)        cACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGTCGCATCCGGCAGTTGt                                                                                                      <  2:216469/140‑1 (MQ=255)             aCCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGTCGCATCCgg                                                                                                             >  2:305189/1‑128 (MQ=255)             aCCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGTCGCATCCgg                                                                                                             <  1:305189/128‑1 (MQ=255)                 gCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGTCGCATCCGGCAGTTGTACGCAGGTg                                                                                             >  2:7209/1‑140 (MQ=255)                               aTAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGTCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCt                                                                               >  2:382111/1‑140 (MQ=255)                                  aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGTCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                            <  1:7209/140‑1 (MQ=255)                                   gCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGTCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgcg                                                                           <  1:247479/140‑1 (MQ=255)                                     cGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGTCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGcgcg                                                                         <  1:239899/140‑1 (MQ=255)                                      gATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGTCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGt                                                                        <  1:390648/140‑1 (MQ=255)                                         tAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGTCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCtt                                                                     >  2:60835/1‑140 (MQ=255)                                            ccGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGTCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                  <  1:420300/140‑1 (MQ=255)                                            ccGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGTCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                  <  1:515968/140‑1 (MQ=255)                                            ccGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGTCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                  <  1:382111/140‑1 (MQ=255)                                                     tCTGGCAGGACCATTTTTTGGGCGCGCGAAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGTCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACg                                                         <  1:397281/140‑1 (MQ=255)                                                       tGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGTCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAg                                                       <  2:426258/140‑1 (MQ=255)                                                             gACCATTTTTTGGGCGCGCGAAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGTCGCATCCGGCAGTTGTACGCAGGTGCATGATGCGAAGCAGGCGCGTCTTATCATGCCTACGAGCCcgcg                                                 >  2:397281/1‑140 (MQ=37)                                                              aCCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGTCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGa                                                >  1:41392/1‑140 (MQ=255)                                                                          gcgcgtgtAGGATTTTGACGGAAGGGACGATTTTGTAGGACGGATAAGGCTTTTAGGTCGCACCCGGCAGTTGTACGCAGGTGCCTGGTGGGACGCTGGCGCTTCTTATGTTGGCTACGGGCCCGCGAATATTTGCGAGc                                    <  2:142415/140‑1 (MQ=255)                                                                                        ttGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGTCGCATCCGGCAGTTGTACGCAGGTGCCTAATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGatat                      <  1:512455/140‑1 (MQ=255)                                                                                              gAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGTCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaaca                >  2:137928/1‑140 (MQ=255)                                                                                                  agaCGATTTTGTAGGACGGATAAGGCGTTTACGTCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCt            <  1:171643/140‑1 (MQ=255)                                                                                                       caaatGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCTGCTTTCCCGATATAAAACAACCTCATTg       >  1:318728/5‑140 (MQ=25)                                                                                                        ttttGTAGGACGGATAAGGCGTTTACGTCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGc      <  1:137928/140‑1 (MQ=255)                                                                                                        aaatGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCTGCTTTCCCGATATAAAACAACCTCATTGc      <  2:318728/137‑1 (MQ=255)                                                                                                                gACGGATAAGGCGTTTACGTCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAAc  >  2:303440/1‑136 (MQ=255)                                                                                                                gACGGATAAGGCGTTTACGTCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAAc  <  1:303440/136‑1 (MQ=255)                                                                                                                                   |                                                                                                                     GAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAAC  >  NC_000913/247351‑247597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑