Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,566,223	C→T	G120D (GGC→GAC)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,566,223	0	C	T	100.0%	76.3 / NA	24	G120D (GGC→GAC)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/13);  total (11/13)

TACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAG  >  NC_000913/3566092‑3566359                                                                                                                                    |                                                                                                                                         tACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGTGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGTCTGGGTCa                                                                                                                                  >  1:288937/1‑140 (MQ=255)               ttCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGTCTGGGTCATTTTCTTCatcaat                                                                                                                    >  2:85247/1‑140 (MQ=255)                     ttGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGTCTGGGTCATTTTCTTCATCAATCAGctc                                                                                                              >  2:284183/1‑140 (MQ=255)                       gAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGTCTGGGTCATTTTCTTCATCAATCAGCTCtt                                                                                                            >  1:528621/1‑140 (MQ=255)                                   gTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGTCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAAcc                                                                                                <  1:11384/140‑1 (MQ=255)                                        aGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGTCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATc                                                                                           >  1:372958/1‑140 (MQ=255)                                              tGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGTCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTcc                                                                                     >  2:301314/1‑140 (MQ=255)                                                    aaCCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGTCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTgcg                                                                               <  1:30561/140‑1 (MQ=255)                                                      ccGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGTCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGcc                                                                             <  1:208193/140‑1 (MQ=255)                                                                             cGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGTCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTa                                                      >  2:255595/1‑140 (MQ=255)                                                                              gCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGTCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCt                                                       >  1:326207/1‑138 (MQ=255)                                                                              gCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGTCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCt                                                       <  2:326207/138‑1 (MQ=255)                                                                                 agagAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGTCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGa                                                  <  1:33601/140‑1 (MQ=255)                                                                                      aTCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGTCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGacaaca                                             <  2:389932/140‑1 (MQ=255)                                                                                        cAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGCTACCGAGGTCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATg                                           <  2:372958/140‑1 (MQ=255)                                                                                               gcaagcCGCCAGACGTCCCAGGCCACCGTTACCGAGGTCTGGGTCATTTTCTTCATCAATCAGTTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTgg                                    >  1:124644/1‑140 (MQ=255)                                                                                                           aCGTCCCAGGCCACCGTTACCGAGGTCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGc                        <  1:160050/140‑1 (MQ=255)                                                                                                                    gCCACCGTTACCGAGGTCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCaaaa               <  1:284183/140‑1 (MQ=255)                                                                                                                        ccGTTACCGAGGTCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTc           >  1:247517/1‑140 (MQ=255)                                                                                                                              ccGAGGTCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGa     >  2:301235/1‑140 (MQ=255)                                                                                                                                gAGGTCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACa   <  2:288937/140‑1 (MQ=255)                                                                                                                                gAGGTCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACa   <  1:85247/140‑1 (MQ=255)                                                                                                                                gAGGTCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACa   <  1:301314/140‑1 (MQ=255)                                                                                                                                 aGGTCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAg  <  1:255595/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                         TACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAG  >  NC_000913/3566092‑3566359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑