Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,756,167	G→A	P115P (CCC→CCT)	aldB ←	aldehyde dehydrogenase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,756,167	0	G	A	100.0%	105.0 / NA	31	P115P (CCC→CCT)	aldB	aldehyde dehydrogenase B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/18);  total (13/18)

ATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCACCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATGGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATCCTGCACCGAGGTGTGCGCCCATTTATCTTTCACTTTGTG  >  NC_000913/3756047‑3756298                                                                                                                         |                                                                                                                                    aTAGGCCACGGTTTCGCTATCAACTTCACTGATCCCACCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGtt                                                                                                                                 >  2:448430/1‑125 (MQ=255) aTAGGCCACGGTTTCGCTATCAACTTCACTGATCCCACCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGtt                                                                                                                                 <  1:448430/125‑1 (MQ=255)     gCCACGGTTTCGCTATCAACTTCACTGATCCCACCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTc                                                                                                              >  2:419867/1‑140 (MQ=255)      ccACGGTTTCGCTATCAACTTCACTGATCCCACCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCa                                                                                                             >  2:335004/1‑140 (MQ=255)        aCGGTTTCGCTATCAACTTCACTGATCCCACCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGc                                                                                                           >  2:163579/1‑140 (MQ=255)          ggTTTCGCTATCAACTTCACTGATCCCACCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTg                                                                                                         >  2:419228/1‑140 (MQ=255)               cGCTATCAACTTCACTGATCCCACCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCt                                                                                                    >  1:43254/1‑140 (MQ=255)                       aCTTCACTGATCCCACCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTc                                                                                            <  1:532720/140‑1 (MQ=255)                             cTGATCCCACCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGtt                                                                                      <  2:147768/140‑1 (MQ=255)                                aTCCCACCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTg                                                                                   <  1:419228/140‑1 (MQ=255)                                          ccTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGtt                                                                                      <  1:20230/127‑1 (MQ=255)                                          ccTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGtt                                                                                      >  2:20230/1‑127 (MQ=255)                                          ccTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGa                                                                         >  1:328251/1‑140 (MQ=255)                                            tGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATc                                                                       >  2:501745/1‑140 (MQ=255)                                              cgcCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCgg                                                                     <  1:419867/140‑1 (MQ=255)                                              cgcCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCgg                                                                     <  2:451879/140‑1 (MQ=255)                                                        cacGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTaaa                                                           <  1:335004/140‑1 (MQ=255)                                                                     aGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCa                                              <  1:418074/140‑1 (MQ=255)                                                                          aaaTGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATc                                         <  1:163579/140‑1 (MQ=255)                                                                          aaaTGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATc                                         <  1:32460/140‑1 (MQ=255)                                                                            aTGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCa                                              >  2:206577/1‑133 (MQ=255)                                                                            aTGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCa                                              <  1:206577/133‑1 (MQ=255)                                                                            aTGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATCCt                                       <  2:129221/140‑1 (MQ=255)                                                                                         aGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATCCTGCACCGAGGtgtg                          <  1:501745/140‑1 (MQ=255)                                                                                                cATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATCCTGCACCGAGGTGTGCGCCCAt                   <  1:484096/140‑1 (MQ=255)                                                                                                cATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATCCTGCACCGAGGTGTGCGCCCAt                   >  2:223424/1‑140 (MQ=255)                                                                                                    cGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATCCTGCACCGAGGTGTGCGCCCATTTAt               <  1:431626/140‑1 (MQ=255)                                                                                                              gTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATCCTGCACCGAGGTGTGCGCCCATTTATCtt            >  2:388721/1‑133 (MQ=255)                                                                                                              gTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATCCTGCACCGAGGTGTGCGCCCATTTATCtt            <  1:388721/133‑1 (MQ=255)                                                                                                              gTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATCCTGCACCGAGGTGTGCGCCCATTTATCTTTCACttt     <  2:43254/140‑1 (MQ=255)                                                                                                                 tCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATCCTGCACCGAGGTGTGCGCCCATTTATCTTTCACTTtgtg  >  2:219473/1‑140 (MQ=255)                                                                                                                         |                                                                                                                                    ATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCACCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATGGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATCCTGCACCGAGGTGTGCGCCCATTTATCTTTCACTTTGTG  >  NC_000913/3756047‑3756298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑