Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,809,588	C→T	P350L (CCG→CTG)	waaA →	KDO transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,809,588	0	C	T	100.0%	89.1 / NA	27	P350L (CCG→CTG)	waaA	KDO transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/12);  total (15/12)

GCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGT  >  NC_000913/3809456‑3809715                                                                                                                                     |                                                                                                                                gCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACt                                                                                                                          >  1:89672/1‑140 (MQ=255)            ggCGAGTTGATGTTACTGTATGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTaa                                                                                                               >  2:229289/1‑140 (MQ=255)                       gTTACTGTATGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTgcg                                                                                                    <  2:89672/140‑1 (MQ=255)                            tGTATGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCgg                                                                                               >  2:38986/1‑140 (MQ=255)                               aTGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTg                                                                                            <  2:202981/140‑1 (MQ=255)                                      tGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAgg                                                                                     >  1:238967/1‑140 (MQ=255)                                      tGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAgg                                                                                     >  1:300122/1‑140 (MQ=255)                                      tGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAgg                                                                                     >  2:151515/1‑140 (MQ=255)                                        ccGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCa                                                                                   >  2:339713/1‑140 (MQ=255)                                        ccGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCa                                                                                   >  2:249987/1‑140 (MQ=255)                                        ccGATCTCGCCTTTGTTGGCGGTTCACTGGATGAACATGGTGGGCATAATCCGCTGGAAGCTGCCTCACACGCTATTCCGGTATTGATTGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCTGGCa                                                                                   >  2:239886/1‑140 (MQ=255)                                                 ccTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTg                                                                          <  1:229289/140‑1 (MQ=255)                                                  cTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGa                                                                         >  1:16442/1‑140 (MQ=255)                                                        tgGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGAttaccg                                                                   <  1:339713/140‑1 (MQ=255)                                                            ggTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGAttaccgttac                                                               <  2:238967/140‑1 (MQ=255)                                                            ggTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGAttaccgttac                                                               <  2:300122/140‑1 (MQ=255)                                                             gTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGAttaccgttacc                                                              <  2:147915/140‑1 (MQ=255)                                                                 aCTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATg                                                          >  1:473077/1‑140 (MQ=255)                                                                         aaCGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACg                                                  <  1:22070/140‑1 (MQ=255)                                                                           cGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCt                                                <  1:249987/140‑1 (MQ=255)                                                                           cGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTACTCCGGTATTGCTGGGGCTGCATACTTTTAACTTTAGAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATGACCGTTACCGATGCCACTACGCt                                                >  1:467004/1‑140 (MQ=255)                                                                             tggtggGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTg                                              >  1:491241/1‑140 (MQ=255)                                                                                             cTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCctc                              >  2:539721/1‑140 (MQ=255)                                                                                                      gCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCAc                     <  1:371000/140‑1 (MQ=255)                                                                                                             acGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGcc              <  1:151515/140‑1 (MQ=255)                                                                                                             acGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGcc              <  2:126068/140‑1 (MQ=255)                                                                                                                         tATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGt  >  1:172150/1‑140 (MQ=255)                                                                                                                         tATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGt  >  1:509689/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                GCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGT  >  NC_000913/3809456‑3809715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑